LOCUS       sequence-195239-        3450 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E6I4Q5
FEATURES             Location/Qualifiers
     source          1..3450
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtacagta taggtctgga tctggggatc tctagcgtgg gctggtctgt gattgatgag
       61 cgaactggga acgtcataga tctgggggtg agactctttt cagccaagaa tagcgagaag
      121 aacctggagc gcagaacgaa ccgcggcggg cgccgcctga tcaggcggaa aaccaaccga
      181 ctcaaggatg ctaaaaagat cctggctgcc gtgggcttct acgaagacaa atctctgaag
      241 aactcctgcc cataccaact ccgcgtaaag ggcctgactg agcctttgag caggggggag
      301 atctataaag tcacattgca cattctcaag aagcggggca tttcctatct ggatgaggtc
      361 gatactgagg ccgccaagga gtctcaggac tataaggaac aagttcgcaa aaacgcccaa
      421 ctgcttacca agtacacccc cggacagatc cagctgcaga ggctgaaaga aaacaatagg
      481 gttaaaacag gtatcaatgc ccagggaaac tatcagttga acgtgttcaa ggtgtccgcg
      541 tatgccaatg agttggccac catcctgaag acacagcagg ccttttatcc caacgagctg
      601 accgacgatt ggattgccct gttcgttcag cccggcatag ctgaggaggc tggcctgatt
      661 taccggaaaa gaccttatta tcacgggcct ggcaatgagg caaataatag cccctacggc
      721 aggtggtctg acttccagaa aacaggcgaa ccggctacaa acatttttga taagctgatc
      781 ggcaaagact ttcaggggga gctgagagct agtggcctgt ccctcagcgc ccagcagtac
      841 aacctgttga acgacttgac caatctgaag attgatggcg aggtccccct cagctccgag
      901 cagaaggagt atattctgac cgagcttatg actaaagagt ttacccggtt tggggtcaac
      961 gacgtggtga agctcctggg cgtgaaaaaa gagcggctta gcgggtggcg gctggataag
     1021 aaaggtaagc ctgaaattca cacgctgaaa ggttaccgaa attggagaaa gatttttgcc
     1081 gaagccggca tcgaccttgc gacgctgcct actgagacta tcgactgcct tgcgaaagtg
     1141 ctcacactca ataccgagag agaaggcatt gaaaatacac tggctttcga gctgccagaa
     1201 cttagcgagt ccgttaaact gctggtcctt gatcgctaca aagaactgag tcagagcatc
     1261 tccacccagt catggcaccg attcagtttg aagactctgc acctgctgat acccgagctt
     1321 atgaatgcta cgtcagagca gaataccctg ctcgagcaat tccagcttaa aagcgatgtg
     1381 cgaaaacgct atagcgagta caaaaagctg cccacaaaag atgtgcttgc cgagatttat
     1441 aaccccacag tcaataagac cgtcagccag gccttcaaag tcatagacgc cctcctggtc
     1501 aagtacggta aggagcaaat taggtatatc accattgaaa tgccgcggga tgataacgag
     1561 gaagacgaga aaaaacgaat caaggagctg catgccaaga attcccaacg gaagaacgat
     1621 agccagtcct atttcatgca aaaatccggc tggagtcagg agaaatttca aacgactatt
     1681 caaaaaaacc ggagatttct cgctaagctc ctgtattact atgaacagga tggcatatgt
     1741 gcatacaccg gtctgccaat cagccctgag ctgctggtca gtgactctac cgaaattgac
     1801 catatcatcc caattagtat ctccctcgat gacagtatta acaacaaagt gctcgttctc
     1861 tccaaggcca accaagttaa aggacagcaa accccttatg atgcctggat ggatggctct
     1921 ttcaaaaaaa tcaacggcaa gttctctaat tgggatgatt accagaagtg ggtggagtct
     1981 aggcacttct ctcataagaa ggagaacaat ctgctggaaa cccggaatat cttcgattca
     2041 gagcaggtgg aaaagtttct ggcgaggaac ctcaatgaca ccaggtacgc ctctaggctg
     2101 gtgttgaata cactccaatc tttttttaca aatcaggaaa ccaaggtgcg ggtggtaaac
     2161 gggagcttca cacacaccct gagaaaaaaa tggggtgccg acctggacaa gactagagag
     2221 actcaccacc accatgctgt tgatgcaaca ctgtgtgccg taactagctt cgtgaaggtc
     2281 agtcgatacc actacgccgt gaaggaagag acaggcgaga aagtgatgag agagatcgat
     2341 ttcgaaaccg gtgaaatagt caacgaaatg tcctactggg aatttaagaa atccaagaaa
     2401 tacgagagaa agacatacca ggtgaagtgg ccgaacttcc gggagcagct gaaacctgtg
     2461 aatctgcacc cccgcatcaa gttctcacac caagtcgata ggaaagctaa cagaaaactg
     2521 tccgatgcca ccatctacag cgtccgcgag aaaaccgaag ttaagacatt gaagagcggg
     2581 aaacagaaaa taaccaccga cgagtacacc atcgggaaga taaaagatat atataccctg
     2641 gacgggtggg aggccttcaa gaaaaaacaa gataaactgc tgatgaaaga tcttgacgaa
     2701 aagacctatg aacgccttct gtccatcgct gagacgaccc ctgactttca ggaggttgag
     2761 gagaagaacg gaaaagttaa gagggttaaa cgctctcctt tcgctgtgta ctgcgaagag
     2821 aacgatatcc ctgccattca aaagtacgca aaaaagaaca acggtcccct cattaggtcc
     2881 ctgaagtact acgatggcaa acttaataaa catatcaaca tcaccaagga ttcccaggga
     2941 cgccccgtag aaaaaaccaa gaatgggagg aaagtgacac tgcaatcctt gaagccgtac
     3001 agatatgata tctaccagga cctggagaca aaagcctact atactgtgca gctctattat
     3061 agtgacctcc gatttgtgga ggggaagtat ggcattacag aaaaagaata catgaaaaaa
     3121 gttgccgaac agacaaaggg ccaggtagtc cgcttctgct tctctcttca gaaaaacgac
     3181 ggcctcgaga tagagtggaa ggattcacag agatacgacg tcaggtttta caacttccag
     3241 tccgccaact ctatcaactt caagggtttg gagcaagaga tgatgcctgc tgagaatcaa
     3301 ttcaaacaga aaccttataa taacggtgcc ataaatctga acatcgctaa gtatggtaaa
     3361 gagggcaaga aactgagaaa gttcaataca gatattctgg gaaaaaaaca ctacctcttc
     3421 tatgaaaagg aacccaaaaa tattataaag
//
