LOCUS       sequence-195241-        3162 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E6MBW6
FEATURES             Location/Qualifiers
     source          1..3162
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaaccaga agtttatact ggggctcgac attgggatta caagcgtggg ttacggcctt
       61 attgactacg aaaccaaaaa tattatagat gccggtgtca ggctcttccc tgaggcaaat
      121 gtagagaaca acgaaggacg gagaagcaaa cgcggttcac ggcggctgaa acgcaggagg
      181 atacataggc tggagcgggt caagaagctg ctggaggatt acaatttgct ggatcagtct
      241 caaattcctc agagcaccaa tccatatgct atacgggtga agggcctgtc tgaggctctg
      301 agcaaagatg aacttgtcat agcacttttg cacattgcaa agcggcgggg tatccacaaa
      361 atcgacgtga tcgattctaa cgacgatgtg gggaatgaac tctccactaa agaacaactg
      421 aacaaaaact ccaagctgct caaagataaa tttgtgtgtc aaattcagct ggagcgcatg
      481 aatgaggggc aggtcagagg tgagaaaaat cggtttaaaa cagccgacat aattaaggag
      541 attatccaat tgctcaatgt gcaaaaaaat ttccatcaac tcgatgagaa tttcatcaat
      601 aaatacattg agcttgtaga gatgcgcaga gagtacttcg agggaccagg aaaaggcagt
      661 ccctatggtt gggaaggcga ccctaaagcg tggtacgaaa cattgatggg gcactgcacg
      721 tactttcccg acgaactgcg ctccgtcaag tacgcctaca gcgcagacct gttcaacgct
      781 ctgaacgatc ttaacaacct ggttattcaa agagatggcc tgagcaagct ggagtaccat
      841 gagaaatatc acatcatcga aaacgttttt aaacaaaaga agaaaccaac actgaaacag
      901 atcgctaatg agattaatgt aaaccccgaa gatatcaagg gttaccgaat caccaaatcc
      961 ggaaagcccc agtttacgga gtttaaactc taccacgatc tcaagtcagt cctttttgac
     1021 caaagtatct tggagaatga ggacgtactg gatcagattg cagagattct gaccatttat
     1081 caagacaagg actctatcaa gtcaaaactt actgaacttg acatccttct gaacgaggaa
     1141 gataaggaga acattgccca gctgaccggt tacaccggaa ctcaccgctt gtcactgaag
     1201 tgtatccgcc tggtccttga ggagcagtgg tactcctcac gcaaccaaat ggaaatcttc
     1261 actcacctga acatcaaacc caagaagatc aacctgaccg cggctaacaa aatccctaaa
     1321 gctatgatcg acgaatttat ccttagccct gtggttaaaa gaactttcgg ccaggcaatt
     1381 aatttgataa acaagataat cgagaaatac ggggtcccgg aggatatcat aattgagctg
     1441 gctcgggaaa acaactctaa agacaagcag aaattcatta acgagatgca gaagaagaac
     1501 gaaaacacca ggaaacggat taacgaaatc atcgggaaat atgggaatca gaatgcgaag
     1561 agactggtgg agaagattcg cctccacgac gagcaggagg ggaaatgtct gtatagcctg
     1621 gaatcaattc cactggagga tctgctgaac aatccgaatc actacgaagt ggaccacata
     1681 attcctcgca gtgtgtcctt cgacaacagc tatcacaaca aagttttggt gaaacaaagc
     1741 gaaaacagca agaaaagcaa tctcacacca tatcagtact ttaattcagg aaaaagcaaa
     1801 cttagctaca accagttcaa gcagcatata cttaacctga gtaagtccca ggatcggatc
     1861 agtaagaaaa agaaagaata tttgctggaa gagcgggata ttaacaaatt cgaagtgcaa
     1921 aaagaattca taaaccgaaa tctggtggat acccgctatg ccacccgaga attgacaaat
     1981 tacctgaaag catactttag cgccaataac atgaacgtga aggtgaagac tatcaacgga
     2041 tcattcaccg actaccttcg caaagtgtgg aagttcaaga aagagagaaa ccatggatac
     2101 aagcaccacg ctgaggatgc acttatcatt gccaacgctg attttctctt caaggaaaat
     2161 aagaagctga aggctgtaaa tagcgttttg gagaagcccg aaatagaatc caaacagctt
     2221 gacattcagg tcgatagtga agacaattac tctgagatgt tcattattcc caagcaagtt
     2281 caggacatca aggatttcag aaattttaaa tattcacata gggtagataa gaaaccgaac
     2341 agacagctga ttaatgacac actgtactct acacggaaga aagataattc cacatacatt
     2401 gtgcaaacta tcaaggacat atatgccaag gacaacacca cactcaagaa acaatttgat
     2461 aagtcacccg agaaatttct gatgtaccag cacgaccccc gcacattcga aaagctggaa
     2521 gtgattatga aacaatatgc aaacgagaaa aatccactgg ctaagtatca cgaagagact
     2581 ggcgaatatc tcacaaagta ctcaaagaaa aacaacggcc ccatagtgaa gagcctgaag
     2641 tatatcggga ataagctggg gagccacctt gacgttactc atcagtttaa gtcctctact
     2701 aaaaaattgg tcaagctcag cattaagccc tacaggttcg acgtatacct cacggacaag
     2761 ggatataaat tcattactat tagctatctg gatgttctca aaaaggacaa ttattactac
     2821 atccctgaac agaagtacga taaacttaaa ctgggtaagg ccattgataa gaacgccaaa
     2881 ttcatcgcct ccttttacaa aaatgacttg atcaagctcg acggcgagat ctacaagatt
     2941 atcggcgtga acagcgatac acggaatatg atcgaactgg acctgccaga catacggtac
     3001 aaggagtact gcgagctgaa taatatcaag ggcgagcctc ggatcaagaa aaccatcgga
     3061 aaaaaagtca attccatcga aaagctgaca acagatgtcc tgggtaacgt gttcacaaac
     3121 acccagtata ccaaacccca actgctcttt aagaggggta ac
//
