LOCUS       sequence-195243-        3396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E6WZS9
FEATURES             Location/Qualifiers
     source          1..3396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaagaaaa tcctgggtgt cgacctgggc atcactagct ttggatacgc gattcttcaa
       61 gagactggaa aggaccttta taggtgtctg gacaattctg tagtgatgag gaataatccc
      121 tatgacgaaa aatcaggtga gtcaagccag tctatccgca gcacacaaaa gagcatgaga
      181 aggctgatcg aaaagcgcaa aaaaaggatc cgctgtgtgg ctcagactat ggaacgatac
      241 ggtattcttg actacagcga aaccatgaaa atcaatgatc caaagaataa tcctataaag
      301 aaccggtggc aactgcgcgc cgtggacgcc tggaaaaggc ccctcagccc tcaggaactg
      361 ttcgcaatct tcgcccacat ggcaaaacac agagggtaca aatctattgc aacagaagac
      421 ctcatctacg agctcgagct tgaactggga ctcaacgatc ctgaaaagga gtctgagaag
      481 aaagcagacg agcgccgaca ggtgtataac gctctccgac acttggagga attgaggaaa
      541 aagtatggag gagaaacaat cgcacaaaca attcacaggg ccgtggaggc gggagatctt
      601 agaagttaca ggaaccatga cgactatgaa aagatgatca gacgcgaaga tatagaagaa
      661 gagatcgaga aggtgttgct taggcaggcg gagcttggcg ctttgggctt gcccgaggag
      721 caagtctcag agctcataga tgaactcaag gcctgtatca ccgaccagga gatgcctaca
      781 atcgatgagt ctctgtttgg taaatgcacg ttctacaagg atgaactggc cgccccagca
      841 tactcttacc tgtacgattt gtaccgcttg tataagaaac tcgcggattt gaacatcgat
      901 ggttacgagg ttactcagga ggacagggaa aaggtgatcg aatgggtcga gaagaagatt
      961 gcccagggca aaaatctgaa gaaaataacc cataaggatc tgcggaaaat cctgggcctt
     1021 gcccccgagc agaagatctt cggcgtggaa gacgagagaa ttgtcaaggg caaaaaggaa
     1081 ccccggacct tcgtgccctt cttttttttg gcagacattg caaagtttaa agagctcttc
     1141 gcgagtatcc agaaacaccc agacgctttg cagattttcc gcgagctcgc agaaattctc
     1201 cagaggtcca aaactccaca ggaggccctg gatcggctcc gggcactgat ggccggcaag
     1261 ggcattgata cggacgacag ggaacttctg gagctgttta agaacaaaag gtccgggaca
     1321 agagagctct ctcatagata tattctcgag gccttgccgc tgttcctgga aggatacgac
     1381 gagaaggagg tgcagcgcat tctgggcttc gatgatcgcg aagactacag ccgatatcct
     1441 aagagcctgc gacatctgca cttgagggag ggaaacctct tcgaaaaaga agagaaccca
     1501 atcaataacc acgccgtaaa gagcctggcc tcttgggctc tgggcctgat cgcggatctc
     1561 tcttggcggt acgggccttt cgatgaaatc atattggaaa ccaccagaga cgcgctgccc
     1621 gaaaaaattc ggaaagaaat cgataaggcg atgagagagc gcgagaaggc tctggacaaa
     1681 atcatcggaa agtataagaa ggagttcccc tccatcgata aacgcctggc ccgcaagatc
     1741 cagctgtggg agcggcagaa ggggctcgac ctttatagcg gcaaggtcat caatttgagc
     1801 caactcctgg acggcagtgc cgatattgag cacattgtac cccagagcct cggggggctc
     1861 tccactgatt acaatactat cgtcaccctc aagtcagtta acgccgcaaa gggcaaccgc
     1921 cttccagggg attggctggc cggcaaccct gactaccgcg agagaattgg gatgctctct
     1981 gagaagggac ttattgactg gaaaaagaga aagaatcttc tggctcagag cctggacgag
     2041 atttataccg agaacactca ctcaaagggt attcgcgcca cctcctacct ggaagctctg
     2101 gtcgcccaag tgctgaaaag gtattacccc ttccccgatc cagagctgcg aaaaaacggg
     2161 attggtgttc gaatgattcc ggggaaagta acctccaaaa ctaggagcct tcttggcatc
     2221 aaatctaaaa gtagagagac taacttccac cacgctgagg acgccctgat attgtccact
     2281 ctgacccgcg ggtggcaaaa tcgattgcac cgcatgctgc gagataacta tggaaagagt
     2341 gaggccgagc tgaaggagct gtggaagaag tacatgccgc atatcgaggg cttgaccctg
     2401 gccgactaca tcgatgaggc gtttcgaagg ttcatgagca agggagaaga aagccttttt
     2461 tatcgcgaca tgttcgatac cataagatcc atctcatact gggtagataa gaagcccctt
     2521 agtgcgtcaa gccataaaga aaccgtgtat agcagcagac atgaggttcc caccctgcga
     2581 aagaatattc tggaagcatt cgactctttg aacgtgatta aggaccggca caagttgacc
     2641 actgaggaat tcatgaaacg gtacgacaaa gagatccgcc agaaactctg gctgcatcgc
     2701 attggtaata ctaacgacga gagctaccgc gccgtggagg agagggccac ccagatcgct
     2761 cagatactca ccaggtatca gttgatggat gcacagaacg ataaggaaat cgatgaaaag
     2821 tttcagcaag cactgaagga gctcattacc agtcctatcg aggttaccgg aaaactgctg
     2881 aggaagatgc gatttgttta cgacaaactc aacgcgatgc agattgacag gggtctggtg
     2941 gaaacagata agaacatgtt gggaatccac atcagcaaag gtcctaatga gaaattgatc
     3001 ttccgccgga tggatgtcaa caatgcacac gagctgcaaa aggagcgatc aggaattttg
     3061 tgctacctca acgagatgct gttcatcttc aataagaagg gtctgataca ctacgggtgc
     3121 ctgaggtctt atctcgaaaa ggggcagggt agtaagtaca ttgccctctt taacccacgc
     3181 tttccagcta atcccaaggc tcagccctct aagttcacgt cagatagcaa aatcaaacag
     3241 gtggggatcg ggagcgctac aggaatcatc aaggctcact tggacctcga cggccacgtg
     3301 agaagttatg aagtgttcgg gacactgccc gaggggtcta ttgagtggtt taaggaagag
     3361 agtggatacg gccgggttga ggatgacccg catcat
//
