LOCUS       sequence-195245-        4266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E7H313
FEATURES             Location/Qualifiers
     source          1..4266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgacacaaa gtgagcggcg ctttagctgt agcatcggaa tagatatggg ggctaagtat
       61 accggcgtat tctatgcgct ctttgaccgc gaagagctcc ccaccaatct caactcaaag
      121 gcaatgaccc ttgttatgcc cgaaaccggc ccaagatacg tgcaggcaca acgcaccgcc
      181 gttcggcaca ggctgcgggg tcagaaacgg tatacactgg ctcgcaaact tgcattcctc
      241 gtagtggatg acatgatcaa gaagcaggag aagcgcctga cagatgagga gtggaagcga
      301 gggcgcgaag ctctcagtgg gctgttgaaa aggagaggtt acagtaggcc gaatgccgat
      361 ggtgaggatc tgaccccact cgagaacgtt agggcagatg tcttcgccgc acatccagcg
      421 tttagcactt acttttctga ggtgcggagc ctggcagagc agtgggaaga gttcaccgcc
      481 aatatcagta atgtcgagaa gtttctgggc gatcccaaca tcccggccga caaagagttt
      541 atcgaattcg cagtggctga agggctgatc gataaaaccg agaagaaggc ctaccaatct
      601 gcactgtcca ccttgagagc taacgccaac gtcctgactg gcttgaggca aatgggacat
      661 aagcccagaa gcgaatactt taaagcgatt gaagccgatc tgaagaagga ctctcggctg
      721 gccaagatta acgaggcatt tggaggagct gaaaggctgg ctaggctctt gggaaacctg
      781 agtaacctgc agctgagggc cgagagatgg tattttaatg ctcctgacat tatgaaggat
      841 cgcggctggg aaccagatcg gtttaagaag actttggtgc gcgcctttaa gtttttccat
      901 ccagcaaaag accagaataa acagcacctg gaactgatta agcaaataga gaacagcgag
      961 gatatcatcg aaaccctttg caccctcgat cccaacagaa ccatcccccc atatgaggac
     1021 cagaacaacc ggcggccccc cctcgatcag actctgctcc tctctcccga aaaacttacc
     1081 cgccagtatg gcgaaatttg gaagacctgg agcgcccggc ttacttctgc tgagcccacc
     1141 ctggcccccg ccgcggaaat tcttgaacgg tcaacagaca ggaaatcaag agttgccgtt
     1201 aacggtcatg agcctctccc aacgctcgca tatcaactga gttacgcact ccagcgagca
     1261 tttgacagat ccaaagccct cgatccctac gctctgcggg ctttggcggc agggagcaaa
     1321 tccaacaagc tgacttctgc ccgcaccgcc ctcgaaaatt gcattggagg gcagaatgtt
     1381 aagactttcc tggactgcgc ccgccggtac tatcgcgagg ctgacgacgc caaggttggc
     1441 ttgtggtttg ataacgcaga cggactgctg gaaaggagtg acctccaccc acccatgaaa
     1501 aagaaaatac ttccgcttct cgtagcgaac atcctgcaaa cagacgagac aacagggcaa
     1561 aagttcttgg atgaaatttg gcgcaagcag atcaagggaa gagaaaccgt cgcgtccagg
     1621 tgcgcccgga ttgaaaccgt gcgaaagtcc tttggcgggg gattcaacat cgcatacaac
     1681 acagcccagt atcgcgaagt aaataagctt cctcggaatg cacaggataa ggagctgctg
     1741 acaattcgcg atcgggttgc ggagactgcc gattttattg cagcgaatct cggtctttct
     1801 gacgagcaaa agagaaaatt cgctaacccc tttagtctgg cacagtttta taccctgatt
     1861 gagacagaag tcagcgggtt ctctgcgacc acactggcag tgcatctcga aaacgcatgg
     1921 cgcatgacta taaaggatgc ggtaatcaac ggggaaaccg ttcgagcagc gcaatgcagc
     1981 aggctgccag ccgagacagc acgcccattt gacggcttgg tcaggagact ggtcgatagg
     2041 caggcgtggg aaatagccaa gcgggtttcc acagatatcc agtctaaggt tgacttttct
     2101 aatggaatcg tagacgttag catcttcgtg gaagagaata agtttgagtt ttccgcctcc
     2161 gtcgcggatc ttaagaaaaa caagcgggtt aaggacaaga tgttgagcga ggccgagaag
     2221 ctggagacac ggtggctgat aaagaatgag cgcataaaga aagcaagccg cggaacctgc
     2281 ccctacaccg gcgaccgctt ggccgaagga ggagagatcg accacattct cccgagatcc
     2341 ctgattaaag atgcaagagg aatcgtgttc aatgcagagc ccaacctgat ctatgcctcc
     2401 tccaggggca atcagttgaa gaagaaccag cggtatagcc tttcagacct gaaggcaaat
     2461 taccggaatg agatcttcaa gacatccaac attgccgcca tcactgccga gatcgaagac
     2521 gtggtcacaa agctgcagca gacccaccgc ctgaagtttt tcgatctcct gaacgagcat
     2581 gaacaggatt gtgtcaggca cgccctgttt ctggatgacg ggtcagaggc tcgcgatgca
     2641 gtcctggaac tgcttgccac tcagcggcgc acgagggtga acggtacaca gatttggatg
     2701 attaaaaatc ttgctaacaa gattcgggag gaactgcaga actggtgtaa aacaaccaac
     2761 aataggctgc actttcaggc agctgcgacc aatgtgtctg acgcaaaaaa cttgaggctg
     2821 aagctggccc aaaatcagcc agacttcgaa aaacccgaca ttcagccgat cgcctcacat
     2881 tcaattgatg cactgtgctc attcgcagtg ggctcagcag atgctgagcg agatcagaac
     2941 gggttcgatt atttggacgg caagaccgtg ctggggctct atcctcaatc atgtgaggta
     3001 atacatctcc aggccaagcc acaagaggaa aagagccact tcgattctgt cgccatattc
     3061 aaagaaggta tctacgcgga acaatttttg cctatattca ctcttaatga aaaaatttgg
     3121 ataggctatg agacgttgaa tgcgaagggg gagcggtgtg gtgctattga agtgagtggc
     3181 aagcagccca aggagctgct ggagatgctc gccccatttt tcaataaacc tgtcggtgac
     3241 ctgagtgctc acgccacata tcgaatcctg aaaaagcctg cctatgagtt tctggcgaaa
     3301 gcagccttgc agcccctcag tgccgaagaa aaacgcctcg cagcccttct cgacgctctc
     3361 cggtattgta cttctcggaa gtcactgatg tctctgttta tggccgctaa cggaaaatct
     3421 cttaaaaagc gcgaggacgt gctcaagcct aaactctttc aactgaaggt agagctgaag
     3481 ggggaaaaaa gctttaaact gaatggcagt ctgacattgc cggtgaagca agactggttg
     3541 cggatctgcg attcaccaga actggctgac gcattcggga agccctgtag tgctgacgag
     3601 ttgaccagta agcttgctcg catctggaaa cgaccagtga tgagggactt ggcgcacgca
     3661 cctgttcgca gagagttcag cctgcctgct atcgataatc cctccggggg atttcgaatt
     3721 aggaggacaa atctttttgg taacgagttg tatcaggtgc acgcaattaa tgccaagaag
     3781 tatagggggt tcgccagtgc tgggtccaat gtagattggt ctaaaggaat tctgttcaac
     3841 gaattgcagc atgagaacct cacagaatgc gggggaagat tcatcacctc tgcagacgta
     3901 accccaatgt ccgagtggag gaaggtcgtg gctgaggata acctttccat ctggattgcc
     3961 ccaggcacag aagggcggag gtacgtgagg gtggagacaa cctttattca agcttctcat
     4021 tggttcgaac agagcgtgga gaactgggca attacctccc ccctgtcact ccctgcctcc
     4081 ttcaaagtag ataagcccgc cgaatttcag aaggctgtgg gcaccgagct ttcagagctg
     4141 ctggggcaac ctcgcagcga gatttttatt gagaatgtag gaaacgctaa acacattcgc
     4201 ttttggtata ttgtggtgtc ctccaataag aagatgaatg agagttataa caatgtgagt
     4261 aagtca
//
