LOCUS       sequence-195247-        3981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E7MR72
FEATURES             Location/Qualifiers
     source          1..3981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaaggac aaatgaaaaa caacggcaac aacttgcaac agggcaatta ttatcttggc
       61 ctggacgtgg gcacttcatc cgtaggctgg gccgtgacgg acaccgacta caatgttctg
      121 aaattccgag gcaaaagtat gtggggtgcc cgactgttcg atgaggctag cactgcggag
      181 gaacgccgga cacatagggg caacagacgc agactggcta gacggaagta ccgcctgctg
      241 ttgctggagc agctgtttga gaaggagatc cggaagattg acgacaactt ttttgtgcgg
      301 ctgcacgagt ctaatctgtg ggctgatgac aagagtaagc catccaagtt cctcctcttc
      361 aacgatacaa actttactga taaggattat cttaagaagt atcctactat ttatcatctc
      421 agatccgacc tgatccataa tagcacagag catgatatta gactggtctt tctcgcactt
      481 caccacctta tcaaatacag gggccatttc atctacgaca actctgccaa tggggacgtg
      541 aagacgcttg acgaggccgt gtccgacttc gaagagtacc tgaatgaaaa cgacatcgaa
      601 ttcaatatcg agaataagaa ggagttcatt aacgtgctta gcgataagca tctgaccaaa
      661 aaagaaaaaa agatcagcct taagaaactt tatggagata ttaccgacag tgagaacatt
      721 aatatttcag tactcataga gatgctgtcc ggcagcagta tcagcctcag caatctgttt
      781 aaagatattg aatttgacgg caagcagaac ctgagcttgg attcagacat tgaagagacg
      841 cttaacgacg tggtggacat cttgggggat aacattgacc ttttgatcca cgctaaagag
      901 gtttatgaca tcgcagtcct cacctcaagt ctgggaaaac acaagtacct gtgtgacgca
      961 aaggtggaac tcttcgaaaa aaataagaaa gacctgatga tccttaaaaa atacatcaaa
     1021 aagaaccacc cggaggacta caaaaagatc ttttcctctc caaccgagaa gaaaaactac
     1081 gccgcctact cacagaccaa ttcaaagaac gtgtgttctc aggaagaatt ctgcctcttc
     1141 ataaaaccct acatccggga tatggtgaag tccgagaatg aagatgaggt aaggatcgcg
     1201 aaggaggttg aagacaagag cttcttgact aagcttaagg gcaccaacaa ttctgtggta
     1261 ccctatcaga tccatgagcg cgaactgaat caaattctta aaaatatcgt cgcatacctt
     1321 ccttttatga acgacgagca ggaagacatc tcagttgtag acaaaatcaa attgattttc
     1381 aagttcaaga tcccttacta cgtggggcct ctgaatacca aaagtactcg gtcctgggta
     1441 tacagatctg acgagaagat ttacccctgg aactttagca acgtcatcga tctggataaa
     1501 accgcacatg agtttatgaa ccgattgata ggtcgatgta cttacaccaa cgacccggtg
     1561 ctgcctatgg actccctctt gtattccaaa tacaatgtct tgaacgagat caatcctatc
     1621 aaggtcaacg ggaaagccat tccagtagag gttaagcagg ctatttatac agatttgttt
     1681 gaaaactcta aaaaaaaggt gacacgaaaa tcaatttaca tctacctgct taagaatgga
     1741 tatatcgaaa aggaagatat tgtaagtggc attgatatag aaattaaatc taagctgaag
     1801 agccaccacg actttaccca aattgtccag gagaacaagt gtacacccga ggaaatcgaa
     1861 cgcataatca agggtattct cgtgtattcc gacgacaagt ctatgctgcg cagatggttg
     1921 aagaacaata ttaaggggct gtctgagaac gacgtcaagt acttggccaa gctcaattat
     1981 aaggagtggg gccggcttag taagaccctg ctgacagaca tctacacgat caaccccgag
     2041 gacggggagg cgtgttcaat cctcgacatt atgtggaata ccaatgccac actcatggaa
     2101 atactcagta acgagaaata ccagtttaaa cagaacatcg aaaactacaa agctgagaac
     2161 tacgacgaaa agcagaatct gcacgaggaa ttggatgaca tgtacatctc acccgccgcc
     2221 cggcggtcta tctggcaggc gcttcgcatt gtcgatgaaa tcgtcgatat caagaagagt
     2281 gcccccaaga agatctttat cgagatggca agggaaaaga agagcgctat gaaaaagaaa
     2341 cgcactgaaa gcaggaaaga tactctcctc gagctctaca agtcttgtaa gagtcaggcc
     2401 gacggtttct acgatgagga gttgtttgaa aaacttagca acgagtcaaa tagccggctg
     2461 agacgcgacc agctttacct ctactacacc caaatgggtc gatcaatgta cacaggcaaa
     2521 agaatagact tcgacaaact gatcaacgac aagaacactt acgacataga tcatatatat
     2581 cctcgaagca aaataaaaga tgatagcatc actaaccggg tgctcgttga gaaggacata
     2641 aatggggaga aaaccgacat ttatcccatt tcagaagaca taaggcagaa gatgcaacct
     2701 ttttggaaaa ttctcaaaga gaaagggttg atcaacgaag aaaagtacaa acggctgacc
     2761 aggaactacg agcttactga cgaggagctg agtagcttcg tcgcccgaca acttgtggaa
     2821 acccagcaga gcacaaaagc actggccaca ttgctgaaga aggaataccc cagtgcaaag
     2881 atcgtctaca gcaaggccgg gaatgtgtct gaattcagga acagaaaaga caaggaactg
     2941 ccaaagttcc gcgaaatcaa cgacctccac catgctaagg acgcatactt gaacatcgtg
     3001 gtgggcaatg tgtacgatac taagtttacc gagaaatttt ttaacaacat ccggaacgaa
     3061 aattatagcc tcaagagggt gttcgatttc tctgtgccag gggcctggga cgcaaaaggg
     3121 tccacattta atacgataaa gaaatatatg gctaagaaca atcccattat cgcgtttgca
     3181 ccttatgagg tcaaaggcga gctgttcgac cagcaaatcg ttcctaaggg aaagggccaa
     3241 tttcccatta agcagggaaa agacatcgag aagtacggcg ggtacaataa gctctcaagc
     3301 gctttcctgt tcgcagtgga gtataaggga aagaaagcca gggagcgctc cctcgaaaca
     3361 gtgtacatca aagacgtgga gttgtacctt caggatccca ttaagtattg tgaatccgtg
     3421 ctgggcctta aggaaccaca gatcattaag ccaaaaatcc tgatggggtc cctgttctcc
     3481 atcaataaca agaagctggt ggtcaccggg cggagcggca aacagtatgt gtgccaccac
     3541 atctaccagc tcagcattaa tgacgaagac tcacaatacc ttaagaacat cgccaagtac
     3601 cttcaggaag agcccgacgg gaatattgaa cggcagaaca tcctgaatat taccagcgtc
     3661 aataacatta agcttttcga tgttctgtgc actaaattca acagcaacac atacgaaatt
     3721 attttgaatt cactcaaaaa cgatgtcaat gaggggcgcg agaagttcag cgagctcgac
     3781 attctcgaac agtgtaacat tctgctccaa ctgctgaaag ctttcaaatg taacagggag
     3841 tcctctaatc tggagaagct caacaacaag aagcaggcag gggttatcgt aatcccacat
     3901 ctttttacca agtgctccgt atttaaagta atacaccagt ccataacagg cctgttcgaa
     3961 aaggagatgg atctgctgaa a
//
