LOCUS       sequence-195249-        3468 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E9S7M7
FEATURES             Location/Qualifiers
     source          1..3468
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggggaact actacctggg cttggacgtc ggaataggct caatcgggtg ggccgtcatc
       61 aacatcgaga agaagcgcat tgaggatttt aatgtccgga tttttaagtc tggcgagata
      121 caggagaaga accggaatag tcgggcgagt caacagtgta gaagatcccg cggtctgcga
      181 cgactgtatc gccggaagtc acatcgcaag ctgaggctca agaattatct gtcaatcatt
      241 gggctgacca ctagcgagaa gatcgattac tattatgaga ctgctgataa taacgtgata
      301 caacttcgaa ataaaggact ctctgagaag ctcactccgg aagagatcgc cgcctgtctg
      361 attcacatct gcaataatag ggggtacaag gacttctacg aggtgaatgt tgaggatatt
      421 gaggatccag acgagcgcaa cgagtacaaa gaggaacacg acagtatcgt actgattagc
      481 aacctcatga atgaaggcgg gtactgtacc cctgctgaaa tgatctgcaa ctgcagggag
      541 tttgatgaac caaacagtgt gtacagaaag ttccataact ccgccgctag taagaatcat
      601 tatttgatca cgcgacatat gctcgtgaaa gaggtcgacc ttatcctgga gaatcagagc
      661 aagtattacg gtattctcga cgacaagacc attgctaaaa tcaaagatat tatctttgcc
      721 cagcgggatt ttgagatcgg gcccggcaag aacgagcggt tccggcggtt cacaggttat
      781 ctggatagta ttggtaaatg ccagtttttc aaggaccaag aacgcggttc tcggttcaca
      841 gtgattgcag acatttacgc atttgtgaat gttctgagcc agtacacgta cactaacaat
      901 aggggagagt ctgtattcga taccagcttt gccaacgacc tgatcaacag tgcactcaaa
      961 aacggatcaa tggataaacg cgagctgaag gctattgcca agtcctacca tatagatatc
     1021 tctgataaga attccgacac aagcctgact aagtgcttca aatatatcaa agtcgtgaag
     1081 cctcttttcg aaaagtatgg atatgactgg gataaactga ttgagaatta taccgacacc
     1141 gacaataatg tgctcaacag gatcgggatt gtcctttctc aggcacagac cccaaagcgg
     1201 cgacgcgaga aactcaaggc tctgaatata gggttggacg atggcctgat caatgaactg
     1261 accaaactga aactcagcgg cactgcgaac gtgtcctaca agtatatgca ggggagcatt
     1321 gaggccttct gcgagggcga tctctatgga aaatatcagg ccaaatttaa caaggagatt
     1381 ccggatattg atgaaaacgc caaacctcaa aaactgcctc ccttcaagaa cgaggacgac
     1441 tgtgagttct tcaaaaaccc agtggtgttc cggtctatca acgaaaccag gaagctcatc
     1501 aatgcgataa tagacaagta cggatatccc gcggccgtga acattgagac ggcagacgag
     1561 ctcaacaaga cattcgagga ccgggctatt gacaccaagc gaaacaatga taaccagaag
     1621 gaaaatgatc gcatcgttaa agaaatcata gaatgcataa aatgcgacga agttcacgca
     1681 agacacctga ttgagaagta caagctgtgg gaggctcagg aaggcaaatg tttgtactct
     1741 ggggaaacga tcaccaagga ggatatgctt agggacaagg acaaactctt cgaagtggac
     1801 cacattgtcc cttatagtct catccttgac aacaccatta ataataaagc tctcgtttat
     1861 gccgaggaaa atcaaaaaaa aggccaaaga acacctctga tgtatatgaa tgaggctcag
     1921 gccgccgact accgagttcg agtgaacacc atgttcaaaa gcaaaaaatg ctctaaaaag
     1981 aagtaccagt acctgatgct cccggacctg aacgatcagg aactcctcgg aggatggaga
     2041 tctcggaact tgaacgacac gcggtatatt tgcaagtacc ttgtgaacta cctgcgcaaa
     2101 aacttgcgct ttgatcggag ctacgaatca agtgacgaag acgacctcaa gatccgcgac
     2161 cactatcgcg tgttccccgt gaaatctcgc tttacgtcta tgtttcggcg gtggtggctt
     2221 aacgagaaaa catggggccg ctacgataaa gctgagctga agaagttgac ctacctcgac
     2281 cacgccgcag acgctatcat tatcgcgaat tgtaggcccg aatatgtagt gctcgccgga
     2341 gagaagctca aactcaataa gatgtatcac caggcgggaa aaaggatcac ccccgagtac
     2401 gaacaatcca agaaggcctg cattgacaat ctgtacaaac ttttccgcat ggatagaaga
     2461 actgctgaaa agttgctcag cggacacggc cgcttgactc ctatcattcc taacctgtcc
     2521 gaggaggtcg acaagcgcct gtgggacaag aatatctatg aacagttctg gaaagacgac
     2581 aaggataaga agagctgcga ggaactttac cgggagaacg tcgcatcact gtacaaaggg
     2641 gatcccaagt ttgccagcag tttgagtatg cccgtcatta gcctgaagcc tgaccataaa
     2701 tacagaggga ctataacagg agaggaagcc atccgagtca aagaaattga tggcaagctg
     2761 ataaagctga aacgcaagag tatcagcgaa ataaccgccg agtccatcaa tagcatctat
     2821 acagacgaca aaatcttgat tgattcactg aaaaccatat tcgaacaggc tgattataaa
     2881 gatgttgggg actatttgaa gaagaccaat caacactttt tcactacctc aagtggtaag
     2941 agagtcaata aagtaaccgt catcgagaag gttccaagca ggtggctgag aaaggagatt
     3001 gatgacaata atttctctct gttgaacgac agctcttact actgcattga gctgtacaag
     3061 gattcaaagg gcgacaacaa tctgcagggg atcgcaatgt ccgacattgt gcatgaccgg
     3121 aagacaaaga aactttatct taagccagac tttaattacc ccgatgacta ctacactcat
     3181 gtcatgtata ttttccccgg tgactatctg cggattaaat ctacgtcaaa aaaatccgga
     3241 gagcaactga agtttgaggg gtatttcatt agcgttaaaa acgtcaatga gaactcattc
     3301 cgatttattt ccgacaataa gccgtgtgca aaggataaac gcgtctcaat tacaaagaag
     3361 gatatcgtga tcaagctggc tgtggacctt atgggcaagg tacagggcga gaacaacggt
     3421 aagggaattt cttgtgggga gccacttagc ctgcttaagg agaagaat
//
