LOCUS       sequence-195251-        4284 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-F3QMY7
FEATURES             Location/Qualifiers
     source          1..4284
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggtaaga cacacatcat cggcgtcggt cttgacctgg gcggaaccta taccggtacc
       61 tttataacct cccatcccag tgacgaagcc gagcaccgcg atcatagctc tgcatttacc
      121 gtggtgaact ccgagaagct ctcttttagc tctaagtcac ggacagccgt tcgccatcgg
      181 gtacggagct acaaggggtt tgacctgaga cggagattgc tcctgctcgt ggctgagtac
      241 caacttctgc agaaaaagca aaccctggcg ccagaagaac gcgaaaacct ccgcatagcc
      301 cttagtgggt acctcaagag acgggggtac gcaaggacgg aggccgaaac cgacacttcc
      361 gtcctcgaat ctttggatcc ttccgtattc tcatctgccc caagttttac taacttcttc
      421 aatgactcag agcccctcaa tatccagtgg gaagccatcg ccaactcacc agaaactaca
      481 aaggcactca acaaggagct cagcggtcag aaagaggctg attttaaaaa atatatcaag
      541 acatctttcc cagagtactc cgccaaggaa atcttggcta attacgtcga gggtaggcga
      601 gcaatcctcg acgccagcaa gtacattgcg aaccttcaga gtctggggca caaacacaga
      661 agcaagtacc tgagcgatat tcttcaagac atgaaaaggg atagccggat tacccgcctt
      721 tctgaagcct ttggctctac cgacaatctc tggagaataa ttggcaatat tagtaacttg
      781 caggagcggg cggtacggtg gtatttcaat gacgcaaaat ttgaacaggg gcaggaacag
      841 ctggatgccg tcaagctgaa gaacgtactc gtccgggccc tgaagtatct gagaagcgac
      901 gataaggaat ggagcgcgag ccaaaagcag atcatccagt cactggaaca gtccggggac
      961 gttctggacg tgctggctgg gctggatcca gacaggacaa tccctcctta cgaagaccaa
     1021 aacaatcggc gtcccccaga agaccagaca ctgtacctta accccaaagc tttgtcttcc
     1081 gagtatggag aaaagtggaa gtcttgggca aacaagtttg ctggcgcata tcccctgctt
     1141 acggaggatc tcaccgagat tctcaagaat acggacagga agagccggat caagatccgc
     1201 agcgatgtgc tccccgactc tgattaccgg ttggcgtaca tcctccaacg agcttttgat
     1261 cggtccatcg ccctggatga atgctctatc aggcgaactg cagaggactt cgagaacggg
     1321 gtcgtaatca agaatgagaa attggaggac gtgttgtccg gccaccagct ggaggagttc
     1381 ctggaatttg ccaataggta ttaccaagaa accgctaagg cgaagaacgg gctctggttt
     1441 ccagaaaacg ctcttctcga acgcgccgat ctgcatcccc ctatgaagaa taagattctg
     1501 aatgttatag tggggcaggc tctcggagtt tcaccggcgg agggtacaga ttttatcgaa
     1561 gaaatctgga atagcaaggt taaaggccga tctaccgtcc gcagcatctg taacgcaatc
     1621 gaaaatgagc gaaaaaccta cggtccctac ttcagcgagg actacaagtt cgtcaagacc
     1681 gccctgaagg aaggaaagac agaaaaggaa ctctccaaga aattcgccgc agtgatcaaa
     1741 gtgctgaaga tggtgtcaga ggtggtgccc ttcatcggga aggagctccg gctctccgac
     1801 gaagcccagt ccaaattcga taacctgtat tctctggccc agctgtacaa tctgatcgaa
     1861 accgaaagga acgggttctc caaagtgtct ctcgctgccc acctggagaa cgcatggcgg
     1921 atgaccatga cagatgggtc cgcacaatgt tgtaggctgc ccgctgattg tgtgcgacca
     1981 ttcgatggtt ttatcaggaa ggcgatcgac agaaattcct gggaagtggc taagcggata
     2041 gccgaggaag tgaagaagag cgttgatttc accaatggga cagtcaaaat ccccgttgca
     2101 atcgaggcta acagcttcaa ctttaccgct tcactgactg atctgaagta catacagctt
     2161 aaggagcaaa agctcaagaa gaaactggag gacatccaac ggaacgaaga aaatcaggag
     2221 aaaaggtggc tctcaaagga ggagcgcata cgggccgaca gccacgggat ttgcgcatac
     2281 accggacggc cccttgatga cgtgggtgag attgaccaca ttatcccaag gtccctcaca
     2341 ctgaaaaaat cagagtcaat ctataattca gaggtcaatt tgatattcgt gtcagcccag
     2401 gggaatcagg aaaagaagaa taatatctat ctcctcagta accttgccaa aaattatctg
     2461 gccgccgttt tcggtactag cgacctttct cagatcacga atgagataga gagcaccgtg
     2521 ctccagctga aagccgcagg gcgccttgga tatttcgatt tgttgagcga gaaagaacgc
     2581 gcatgcgcac ggcacgcact tttcctgaat agcgactccg aagcgagaag ggctgtgatc
     2641 gacgtcctgg gttcccgccg caaggccagc gttaatggaa cccaggcatg gtttgtgcgc
     2701 agcatcttca gcaaggttag acaggccctg gctgcctgga ctcaagaaac cggtaacgaa
     2761 ctgatcttcg acgcaatatc cgtgcctgca gcagactcca gcgagatgcg caagagattc
     2821 gctgagtatc gaccagagtt tcggaagcct aaagtacaac ccgttgcttc tcatagcatt
     2881 gatgccatgt gtatctatct ggcggcttgt agcgaccctt ttaaaaccaa gcgcatgggg
     2941 tcccagcttg ccatatacga accaattaac ttcgacaacc tgttcactgg aagttgccag
     3001 gttattcaga atacacccag aaatttcagc gataagacca acatcgctaa tagccccatt
     3061 tttaaagaaa ccatctacgc ggagcggttc ctggatatta ttgtgagccg gggtgaaatc
     3121 ttcatcggat acccgtctaa catgccattt gaggagaagc ctaacagaat ttccatcgga
     3181 gggaaagatc ctttcagtat tctctctgtg ctgggtgcgt atctggataa ggcaccttct
     3241 agcgagaaag agaagctcac aatatacaga gtggtgaaga acaaagcctt cgagctcttt
     3301 agcaaggtgg ccggaagtaa attcacggcc gaggaggaca aggctgctaa gatcctggag
     3361 gcactccact tcgtgacagt gaagcaggac gtggctgcaa cagtttccga tctgatcaaa
     3421 tccaagaagg aattgtctaa agattctata gagaatttgg ccaagcaaaa agggtgcctt
     3481 aagaaggtcg aatacagtag caaggagttc aaatttaagg ggagtctgat tattccagcc
     3541 gcagtggagt ggggaaaggt actttggaac gtgtttaagg agaacaccgc ggaagagctg
     3601 aaggatgaga atgccctgcg caaagccctt gaagctgcct ggccgtcttc tttcgggact
     3661 aggaatttgc acagcaaggc taaacgggta ttctctctcc ctgtggtggc tactcagtcc
     3721 ggagctgtgc gcatccggcg caaaacagcc ttcggagact ttgtgtacca gtcacaggat
     3781 acaaataacc tctattcatc ctttccggtc aaaaatggca aactggactg gtccagtcca
     3841 attatccatc ccgccctgca gaaccggaat ctgaccgctt atggataccg atttgtagat
     3901 cacgacagaa gcattagcat gtccgagttc cgcgaagtct ataataaaga cgatttgatg
     3961 aggatcgaac tggctcaggg cacctcttcc aggcgctacc tgcgggtgga aatgcccggc
     4021 gagaaattcc tggcctggtt tggggagaat tccatttccc tcgggtcctc cttcaagttt
     4081 agcgtgtcag aagtcttcga taacaaaata tataccgaaa acgctgaatt cacaaagttt
     4141 ttgcccaagc cgagggaaga caataaacat aacggcacaa tcttctttga actggtgggg
     4201 cctcgagtca tcttcaatta catcgtggga ggggctgcct catcacttaa agagattttt
     4261 agcgaagccg gtaaagagcg gtcc
//
