LOCUS       sequence-195253-        4263 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-F3UXG6
FEATURES             Location/Qualifiers
     source          1..4263
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgacgaagt tcaacaaaaa ttacagcatc gggctggaca ttggagtgag ttccgtcgga
       61 tatgccgtag tcactgagga ttatcgcgtg cctgcgttca agtttaaagt gctcggcaat
      121 acagagaagg aaaaaattaa gaagaacctg attggttcca ctaccttcgt cagcgcacag
      181 ccagccaaag gaactcgcgt gttccgcgtt aaccgccgca ggatcgaccg ccggaatcat
      241 cgaatcacct acctgaggga tatctttcaa aaagaaattg agaaggtcga caagaacttc
      301 tatcgcagac tggacgagag cttccgggtg ctcggagata aaagcgaaga cctgcagatc
      361 aaacaaccgt tctttggtga caaggagctc gaaaccgcct accacaaaaa gtaccctacg
      421 atctaccacc tgaggaaaca cctggctgat gctgacaaga atagtccggt tgccgatatc
      481 cgggaggtgt acatggcaat tagccatatc cttaagtacc gggggcattt cctgacgctc
      541 gacaagatca accctaacaa tattaacatg cagaatagct ggatcgattt tattgagagc
      601 tgtcaggagg tgttcgacct cgaaatcagc gacgagtcca agaatatagc agacattttt
      661 aagtcaagtg aaaatagaca ggagaaggta aagaagatac tgccttactt ccagcaagag
      721 ttgttgaaaa aagacaagag tatttttaaa cagttgttgc agctcctttt cggactgaag
      781 actaagttta aagactgctt tgaactcgaa gaggagcctg acctgaattt ctccaaagaa
      841 aattatgatg agaacctgga gaacttcctg gggtcacttg aagaggattt ctcagatgtc
      901 tttgcaaagc tgaaagtcct cagagatacc atactcctgt ctgggatgct cacctacaca
      961 ggcgccaccc acgcacgctt cagtgccacg atggtagaga gatacgaaga gcacagaaaa
     1021 gatttgcagc ggttcaagtt tttcatcaag cagaatctgt cagagcagga ttacttggat
     1081 atctttggca gaaagacgca gaatggattt gatgtggaca aggagacaaa aggctatgtg
     1141 ggctacatca caaacaagat ggtcctgact aaccctcaga aacagaagac cattcagcag
     1201 aacttctacg attacatctc cggcaaaata acagggattg agggggcgga atactttctg
     1261 aacaaaatta gcgacgggac cttcctgcgc aagctgagaa cgtctgataa tggggcaatc
     1321 cctaaccaga tccacgccta tgaactggaa aaaatcatcg agaggcaagg gaaggattac
     1381 ccatttctgt tggaaaataa ggataagttg ctgagcatat tgacgtttaa gatcccctac
     1441 tatgtaggac ccctcgccaa ggggagtaac tcccggttcg cttggattaa gagggcgact
     1501 tccagtgaca tcctggacga caatgatgag gacacgcgga atggcaagat taggccttgg
     1561 aattatcaga agctgataaa tatggatgaa accagagatg cattcataac aaacctgatc
     1621 gggaatgaca ttatcctcct gaacgaaaag gttctgccaa aaagatctct tatatacgag
     1681 gaggtcatgc ttcagaacga actcactagg gtgaagtata aagacaagta tggcaaggct
     1741 cacttcttcg actccgagct gcgacagaat attattaatg ggctgttcaa aaataacagc
     1801 aagcgagtga acgccaagtc tctgattaag tacctcagtg acaatcataa ggacctgaac
     1861 gcaatcgaaa ttgtgtccgg ggtggaaaaa gggaaatcat tcaatagtac gttgaaaaca
     1921 tacaatgacc tgaagaccat cttttccgag gaactcttgg atagtgagat ttaccagaaa
     1981 gaactggagg agatcatcaa agtcattact gtcttcgacg ataaaaagag cattaagaac
     2041 tatctcacaa aattcttcgg ccacctggag atcctggacg aggaaaaaat taatcagctg
     2101 agcaaactgc gctattccgg ctggggaaga tacagtgcga agctgctcct ggacattcgg
     2161 gatgaagata ccggcttcaa tctgctccaa ttccttcgaa acgatgagga gaatcggaac
     2221 cttacaaagc tgatttccga caatactctg agttttgagc ctaaaatcaa ggatattcaa
     2281 agcaaaagca ctattgaaga cgatatcttc gacgaaataa aaaagcttgc agggagtccg
     2341 gccattaaga ggggaatatt gaactccatc aagattgttg atgagctcgt gcaaatcata
     2401 gggtatcctc ctcataacat cgtgatagag atggcgcggg agaacatgac gaccgaagag
     2461 ggccagaaga aagctaagac cagaaaaact aagttggaaa gcgcactgaa gaacatcgaa
     2521 aactctcttt tggagaatgg aaaagtgcca cacagcgacg agcagctcca gagcgaaaaa
     2581 ctgtatctgt actacctcca gaacggaaaa gatatgtata ccctcgataa aactggttcc
     2641 cccgcacccc tctacctcga ccagctggat cagtatgagg ttgaccacat cattccatac
     2701 agttttctgc caatagattc tatcgacaac aaagtgctta cccatcgcga gaataatcag
     2761 cagaagctga acaacattcc tgataaggaa accgtggcga atatgaaacc attttgggaa
     2821 aagctgtaca acgccaagtt gatctctcag acaaaatatc agaggctcac tacatccgaa
     2881 aggaccccag acggggtact gacagagtcc atgaaggccg gattcatcga gaggcagctg
     2941 gtcgagacga gacagattat taaacacgtg gcccggatcc tcgataatcg attctctgac
     3001 accaaaataa tcactctgaa gagccagctc attactaact tccgaaacac ctttcacatc
     3061 gcaaagatcc gagagctgaa tgactaccat catgctcatg acgcctactt ggccgtggtg
     3121 gtgggtcaga cactcttgaa ggtgtatcct aaactcgcac cagaactcat ttacggccat
     3181 cacgcgcact ttaaccggca cgaagagaat aaagccacac tgagaaagca cctctactct
     3241 aatataatgc ggttcttcaa taacccagac tccaaagtaa gcaaggacat ttgggactgt
     3301 aacagagatc tgcccatcat caaagatgtg atttataaca gtcagataaa cttcgtcaaa
     3361 cggacaatga taaaaaaggg tgctttttac aatcagaatc cagtgggcaa gttcaacaaa
     3421 caacttgctg ctaacaatag atatcctctc aaaaccaaag ccttgtgcct ggacacatcc
     3481 atctacggtg gctatggacc aatgaacagc gcattgagca ttattatcat cgccgaaaga
     3541 ttcaacgaaa aaaaaggtaa gatcgaaacc gtcaaagagt tccatgacat tttcatcatt
     3601 gattacgaga aattcaacaa caatccgttt cagtttctga atgacactag cgaaaacggc
     3661 ttcctcaaga aaaataatat aaatagagtg ttgggattct atcggatccc caagtatagt
     3721 ttgatgcaga agattgatgg aactcggatg ttgttcgaat ctaaaagcaa tctgcataag
     3781 gcgactcagt tcaaactcac taaaacccag aatgagctgt tctttcacat gaagcgcctg
     3841 ttgacgaaga gtaaccttat ggacctcaag tctaagtccg cgattaaaga gtcccagaac
     3901 tttatcctga agcataagga agagtttgac aacatttcaa accagctgtc cgcgttcagc
     3961 cagaagatgc ttgggaacac cactagcctg aaaaacctca taaaaggata caatgaacgg
     4021 aagatcaagg aaatcgatat ccgagacgaa acgatcaaat atttttacga caacttcatc
     4081 aaaatgtttt ccttcgtcaa aagcggcgcc cccaaagata ttaacgattt ttttgataat
     4141 aaatgtaccg tagctcgcat gaggccaaag cctgataaaa agctcctgaa cgccacactg
     4201 attcaccagt ccatcaccgg cctgtacgag actcggatcg atctctccaa gctgggtgag
     4261 gat
//
