LOCUS       sequence-195257-        3186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-G2ZYP2
FEATURES             Location/Qualifiers
     source          1..3186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..84
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggcagaga aacaacatag atggggcctt gatataggaa ccaactctat cggatgggct
       61 gtcatcgcac tgatcgaggg gcggcccgca ggactcgtgg cgacaggcag ccggattttc
      121 tccgacggac gaaacccgaa agatgggagt tcactggccg tcgagaggcg cggacctaga
      181 cagatgcggc gccgacggga cagatatctt aggaggagag atcggttcat gcaggctctg
      241 atcaacgtgg ggctgatgcc aggtgatgct gcagctcgga aggcactggt tacggagaat
      301 ccctatgtac tccgccagcg cggcttggat caggctctga ccctccccga gttcggcaga
      361 gccctgttcc atttgaatca gagacgaggc ttccagagca acaggaaaac agatcgcgcc
      421 acggcaaagg agagcggaaa ggttaagaat gccatcgccg cctttagagc tgggatgggc
      481 aacgcaagaa ccgtgggcga ggccctcgcg cgccgccttg aggacgggag gcctgtccgc
      541 gcgcgaatgg tggggcaggg gaaagatgaa cattacgaac tttacatcgc cagagagtgg
      601 atcgcccagg aattcgacgc cctgtgggct agccagcaac gatttcatgc cgaagtgctg
      661 gccgacgccg cacgcgaccg gctccgcgcg attctgttgt ttcagcgaaa gttgctgcct
      721 gtgcctgtgg gcaaatgttt cttggagccc aatcaacccc gcgttgccgc cgcacttcct
      781 tcagcccaga gattccgcct gatgcaggag ctcaatcacc tcagggtcat gaccctcgcg
      841 gataaacgcg agaggccttt gtctttccag gagagaaacg acttgctggc gcaactcgtt
      901 gctcggccaa agtgtggatt tgatatgctc cggaaaattg tcttcggagc caataaagaa
      961 gcctatcgct tcaccatcga atcagagaga cggaaggagc tgaaaggttg cgacactgcc
     1021 gcaaagctgg ccaaggtgaa cgctttgggt acgcggtggc aggccctcag tctggatgaa
     1081 caagatagac tggtctgttt gctgctggac ggagagaacg acgccgtttt ggctgatgca
     1141 cttcgggaac actatggcct cactgacgca caaattgata ctctgcttgg cctgagtttc
     1201 gaagatggac atatgagact tgggcgaagc gctcttctgc gggtgctgga cgccctggag
     1261 agcgggcgag acgagcaggg gttgcctctg tcttatgaca aggccgtcgt cgctgccggg
     1321 tatccagccc acaccgctga tctggagaat ggtgagagag atgcgctccc atactatgga
     1381 gaactcctgt ggcgctacac acaggacgca cctactgcca agaacgacgc tgagagaaaa
     1441 tttgggaaaa ttgccaatcc aaccgtgcat attgggctta accagttgcg caaactcgtc
     1501 aatgccctga ttcagagata cggaaagcca gcgcagattg tggtcgaact tgcgagaaac
     1561 ttgaaggcag gcctggagga gaaagagcga attaagaagc agcagacagc taacctcgag
     1621 agaaacgaaa gaattcgcca gaagctgcag gacgctgggg tacctgataa tcgggaaaat
     1681 cgactgagga tgaggctgtt tgaagagctg gggcaaggaa atggactggg taccccatgc
     1741 atttattcag ggcgccagat ctccctgcag aggctcttta gcaatgacgt gcaggtcgat
     1801 cacatcctgc ccttctcaaa aacacttgac gactcattcg caaataaggt cctcgcacag
     1861 catgacgcta atcggtataa aggaaataga ggccccttcg aagcctttgg agccaatcgc
     1921 gacggttacg catgggacga catcagagca agagcggccg ttcttccaag gaataagcgg
     1981 aatagatttg ccgaaaccgc catgcaggat tggctccata acgaaaccga tttccttgcc
     2041 aggcagctta cggacaccgc atatctgtct cgagtggcac gccagtacct cactgctatc
     2101 tgcagtaagg acgatgtcta cgttagtcca ggtaggctta cagccatgct gcgagcaaaa
     2161 tgggggctca atcgggtgtt ggatggtgtt atggaggaac aaggccgccc tgcagtcaag
     2221 aaccgggatg accaccgaca ccacgccatc gacgccgttg tgattggcgc gaccgacaga
     2281 gctatgcttc aacaggtagc caccctggcc gcccgggcta gagaacagga cgcagaacgg
     2341 ctgatcggag atatgcctac cccttggcct aatttccttg aagacgttag agcagccgtg
     2401 gcacgctgtg ttgtgtctca caaaccggac catggacccg agggcgggct ccacaacgat
     2461 acagcatacg gcatcgtggc cggtccattc gaagacggac ggtaccgggt gagacacaga
     2521 gtgtccctct tcgacctgaa acccggggat ctcagcaacg tgcggtgcga cgcgcctctg
     2581 caggccgagt tggagcctat cttcgaacag gatgacgcca gagcacgcga ggtggctctg
     2641 acagcactgg ctgagcgata tcgacagcgg aaggtctggc tggaagagct gatgagtgtg
     2701 ctgcccatcc gacctcgagg ggaggacggt aaaacactgc ccgatagcgc accatacaaa
     2761 gcctacaaag gggattccaa ctactgctac gagttgttca ttaacgagcg gggccgctgg
     2821 gacggcgagc tgatctctac tttcagagcc aatcaagccg cttacagacg attcaggaac
     2881 gatcccgcga gatttcggcg atatactgcc ggagggcggc ccctcttgat gagactttgc
     2941 ataaatgact atatcgccgt tgggacagca gcagagcgga ctatcttccg agttgtgaag
     3001 atgtcagaga acaagattac attggccgaa cactttgagg gaggtacgct gaaacaacgg
     3061 gatgctgaca aggatgatcc attcaaatac ctgactaaaa gtccaggagc tctgagagat
     3121 ttgggagccc ggcggatctt cgtggatctg attggccgcg tgctggatcc cgggattaag
     3181 ggcgac
//
