LOCUS       sequence-195259-        3264 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-G9QLF2
FEATURES             Location/Qualifiers
     source          1..3264
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(660..671)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgaattaca agatgggcct tgacataggt atcgcgtccg tggggtgggc cgtcatcaac
       61 cttgacctga aaagaatcga ggatcttgga gttagaattt tcgacaaggc ggagcaccct
      121 cagaacggtg aatctttggc ccttccccgg agaattgcgc gcagtgctag gaggaggctg
      181 agacgacgga aacatcgcct tgaacggatt agacggctgc tggtgagcga gaatgtgctt
      241 acgaaggaag agatgaactt gctgtttaag caaaaaaagc agatagacgt gtggcagctg
      301 cgcgtggatg ccctggaacg gaagctgaac aacgacgaac tggcacgcgt gcttttgcac
      361 ctggctaaga gaagggggtt caagtctaac agaaagtcag aaagaaacag caaagaaagc
      421 tcagaattcc tgaagaacat tgaggaaaac cagtctattc ttgctcaata taggagcgtt
      481 ggtgaaatga ttgtaaagga ttccaaattt gcttaccaca agcgcaataa gctggacagc
      541 tactcaaaca tgattgcccg ggacgacttg gagagggaaa ttaaacttat cttcgagaaa
      601 cagagagaat ttaacaaccc cgtatgcacg gagcgactcg aggaaaagta tttgaacatc
      661 tggagctccc agcgaccgtt cgccagcaag gaggacatcg agaagaaggt tggattttgc
      721 actttcgagc ctaaggagaa acgggcccca aaggccacat acacattcca gtcctttatt
      781 gtttgggaac acatcaacaa acttaggctc gtcagcccag acgaaaccag ggcgctgaca
      841 gaaatcgaga gaaacctgct ttacaagcaa gcattttcta agaataaaat gacgtattac
      901 gacattcgca agctcctcaa tctcagcgat gatatccatt ttaaggggtt gctgtacgac
      961 cctaaaagct cactgaaaca gattgagaac atcaggtttc tggagctgga ctcctatcac
     1021 aagattagaa aatgcatcga aaatgtgtac gggaaggacg gcatcaggat gtttaacgaa
     1081 accgacatcg atacgttcgg atacgcactt accatcttca aggacgacga agacatcgtg
     1141 gcttatctgc agaatgagta catcaccaag aatggcaaac gcgtaagcaa ccttgctaac
     1201 aaagtgtatg ataagagcct gatcgacgaa ctgctgaatc tctcttttag taaatttgct
     1261 catctctcta tgaaggcgat caggaatatc ctgccttata tggaacaggg tgaaatctat
     1321 tccaaggcct gcgagttggc cggatataat ttcacaggcc caaagaagaa agaaaaggca
     1381 ctgctgcttc cagtgatacc aaacattgcg aatccggttg tgatgcgagc tctcacacag
     1441 tcccgcaagg tggtcaatgc aatcatcaag aagtatggtt cccccgtgtc catccacatc
     1501 gagcttgctc gggacctgtc tcactccttc gatgagagaa aaaagattca gaaggatcag
     1561 accgagaacc ggaaaaaaaa tgaaacagct atcaaacagc tgatagaata tgaactgact
     1621 aagaacccaa caggacttga tatcgttaag tttaaactct ggtctgagca gcagggccga
     1681 tgcatgtaca gcctgaagcc gatcgagttg gagcgactgt tggagcctgg atacgtcgaa
     1741 gtggatcaca tcttgccgta ctcacggtca ctcgatgata gctacgctaa taaggtcctt
     1801 gtactcacta aagaaaacag ggagaaaggc aatcataccc cggtcgagta cctgggactg
     1861 gggtctgagc ggtggaaaaa gttcgagaag ttcgtgcttg ctaataagca gttttctaaa
     1921 aagaaaaagc agaatctgtt gcgcctgcga tatgaagaga cagaggaaaa agaattcaag
     1981 gagcggaacc tcaacgacac ccgctacatt tccaagttct tcgccaactt tattaaagaa
     2041 catctgaagt ttgcggatgg cgatgggggc caaaaagtct acacaatcaa cggaaagatc
     2101 acagctcatc tgagatcccg ctgggacttc aacaaaaacc gcgaggagtc tgatctgcat
     2161 cacgcagtcg atgccgtcat cgtcgcatgc gccacgcagg gcatgatcaa gaagataacg
     2221 gaattttaca aagcaagaga gcagaacaaa gagagcgcta agaagaaaga acccatcttt
     2281 cctcagccct ggccccactt tgccgatgaa ctcaaagcca gactgagcaa gttccctcag
     2341 gagtctatcg aggctttcgc cttgggaaat tatgaccgga aaaagctgga atctctgcgt
     2401 ccagtctttg ttagtaggat gcctaagagg tcagtgacgg gtgcagcgca tcaggaaacc
     2461 ctgcgccggt gcgtcggtat tgatgaacaa tcaggcaaga ttcagacagc cgtaaaaacc
     2521 aagctcagcg atattaaact ggacaaagat gggcattttc ccatgtacca gaaagagtct
     2581 gaccctcgga cctacgaggc tatcaggcag aggctcctgg agcacaataa tgaccccaaa
     2641 aaggcctttc aggaaccctt gtacaagccg aagaaaaatg gggaaccggg gcccgtcatt
     2701 cggaccgtca agatcataga caccaagaac aaagtcgtgc atctcgacgg ctcaaaaact
     2761 gtcgcctaca atagtaacat agtccgcacg gatgtcttcg agaaggacgg caagtactac
     2821 tgcgtcccag tttacaccat ggatatcatg aagggcactc tgcctaacaa agctatcgag
     2881 gccaacaagc cgtactctga atggaaggaa atgaccgagg agtacacctt tcaattcagc
     2941 ctgttcccaa atgacctggt gcgcatcgtc ctgccccgcg aaaagacaat caagacctcc
     3001 acaaatgagg agatcatcat caaggatatt tttgcctatt ataaaaccat agactcagca
     3061 accggaggcc tcgagctgat ttctcacgac aggaacttta gcctgcgcgg cgtcgggagc
     3121 aaaacgctca aaagattcga aaagtatcaa gttgacgtgt tgggaaacat ccataaggtg
     3181 aaaggcgaga agcgggtcgg tctggccgct cccaccaacc agaagaaggg gaaaaccgtc
     3241 gactcccttc agtcagtgag cgac
//
