LOCUS       sequence-195261-        4167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-G9WGU4
FEATURES             Location/Qualifiers
     source          1..4167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcccgag actacagtgt cggcctcgac ataggaactt ctagcgtggg ttgggcagcc
       61 atcgacaata aatatcattt gatcagggcc aagagcaaaa acctgatagg cgtgcgcctg
      121 tttgattccg ccgtgacggc agagaaaaga cgggggtatc gaaccactcg aaggcggctg
      181 tcccggcgcc attggcggct gaggttgctg aacgacattt tcgccggccc actgacggat
      241 ttcggcgacg agaactttct tgcacgcctg aagtactcct gggtgcaccc tcaggatcaa
      301 agcaatcagg ctcacttcgc agccggactg ctgtttgact ccaaggagca agacaaggat
      361 ttttacagaa agtaccccac tatctatcac ctgagactgg cactgatgaa cgacgatcaa
      421 aagcatgacc ttagggaggt gtacctcgcc attcatcatc ttgtcaaata cagaggccat
      481 ttcctgatcg agggcgacgt caaagccgac tctgcctttg acgtgcacac tttcgccgat
      541 gccattcaga ggtacgcaga atccaacaac agcgatgaaa acctgttggg aaaaatcgat
      601 gagaagaagc tgagtgctgc tctcactgat aagcacggtt ctaaaagtca gcgagccgaa
      661 accgctgaga cggccttcga cattttggac ctgcaatcaa aaaaacagat ccaggccata
      721 ctgaagagtg ttgtcggcaa ccaagctaac ctcatggcaa tcttcgggct tgatagcagt
      781 gccatctcaa aagatgagca gaagaattac aagttttcat ttgacgacgc agatattgac
      841 gagaagattg ccgattccga ggctctgttg tccgacaccg agttcgaatt cctgtgtgac
      901 ctgaaggcag cattcgatgg tctgacactg aagatgctgt tgggagatga caaaacggta
      961 tctgccgcta tggtaagacg gtttaatgag catcagaagg actgggagta tatcaaatcc
     1021 catattcgca acgctaagaa tgccggaaat ggcctctacg agaagagcaa aaaatttgac
     1081 gggatcaacg cagcctatct cgccttgcag tctgacaacg aagatgacag aaaaaaagca
     1141 aaaaaaattt tccaggacga gatctcctca gcagatattc ctgatgacgt taaggctgac
     1201 tttctgaaaa aaatcgacga tgaccagttc ctgcccatcc agagaaccaa gaacaatggg
     1261 actatcccac atcagctgca ccgaaacgag ctggagcaga tcatcgaaaa gcagggcatc
     1321 tattatccct ttcttaaaga cacctaccag gagaactcac acgaactcaa caagatcacc
     1381 gcattgatca actttagggt gccttactat gtggggccgc tcgttgagga ggagcagaaa
     1441 atcgctgacg atgggaagaa catccccgat ccgacaaatc actggatggt acgcaaatca
     1501 aacgatacaa tcacgccgtg gaatttgagc caagtggttg acctggacaa gagtggcaga
     1561 aggtttattg agcgcttgac cggaaccgat acatacttga tcggagagcc aacactgccc
     1621 aagaactcac tgttgtatca gaaattcgat gttctccagg aactgaacaa tattcgggtg
     1681 tccggtcgca gactggacat cagagccaag caggatgcct ttgaacacct gttcaaagtg
     1741 cagaaaaccg tctcagccac gaatcttaag gactttctgg ttcaagccgg ttatatttcc
     1801 gaggacacac aaatcgaagg cctggccgat gtgaacggca aaaatttcaa caacgccctc
     1861 acgacataca actaccttgt cagcgtattg ggccgcgagt tcgtggagaa tccaagcaac
     1921 gaggaactcc tggaagagat cacagagctt caaaccgtat tcgaggataa aaaagttttg
     1981 cgccggcagc tcgatcagct ggacgggctc tccgaccata accgggagaa gctgagtagg
     2041 aagcactata ctgggtgggg aagaatttca aagaagttgc tgactaccaa aatcgtacag
     2101 aatgccgaca agattgataa tcagaccttt gatgtgcctc ggatgaacca gtcaatcatc
     2161 gacactctgt acaatacaaa aatgaatttg atggagataa ttaacaatgc cgaggacgat
     2221 ttcggggtca gagcatggat tgataagcag aataccactg atggagacga gcaggacgtt
     2281 tacagtctta tcgacgagct ggcgggccct aaggagatca agagggggat agtccagtct
     2341 ttccgcattc ttgacgacat cactaaagcg gtcggctacg ccccgaaacg ggtatacctg
     2401 gaattcgcgc gcaagaccca ggagagtcac ctgactaata gcaggaagaa ccagctgagc
     2461 accctgctga agaacgcggg actgtccgag ctggtcacac aagtgtctca gtatgatgca
     2521 gctgccctgc agaatgatag actgtatctt tatttcctgc aacaggggaa ggatatgtac
     2581 tcaggcgaaa agctgaatct cgacaatctc tctaactacg atatcgatca tatcatccca
     2641 caggcctata ccaaggataa ctcactcgat aatcgcgtgc tggtcagcaa tatcaccaat
     2701 agacggaaga gcgattcttc taactacctg ccagccctga tagataaaat gcgacctttc
     2761 tggtcagtgc tgagtaagca gggtctgttg agtaaacaca aattcgctaa tctgacccgg
     2821 acgagggatt ttgacgacat ggagaaagaa agattcatcg ctcgaagcct ggtggagact
     2881 aggcaaatta ttaaaaacgt agcgagcctc atcgacagtc atttcggtgg agagacaaaa
     2941 gccgtcgcca tacgctcaag ccttaccgcg gacatgagac gctacgtgga catccctaaa
     3001 aaccgggata tcaatgacta tcatcacgcc tttgacgccc ttcttttcag caccgtaggc
     3061 cagtatactg agaactccgg actgatgaag aaggggcagc tttccgacag cgctggtaac
     3121 cagtataacc ggtatatcaa ggaatggatt cacgccgccc ggctgaacgc ccagtctcag
     3181 cgcgtgaacc cctttggatt cgtggtgggc tccatgagaa acgctgcgcc cggcaaactt
     3241 aatcctgaga ctggcgagat aacaccagag gagaatgcag actggtcaat tgcagacctt
     3301 gactatctgc ataaggtgat gaactttagg aagataacag taacccgaag gctgaaagac
     3361 caaaagggac agctttatga cgagagcaga tacccatctg tcctccacga cgcaaagagc
     3421 aaagctagca tcaatttcga taaacacaaa ccagtcgacc tttatggagg tttctcttca
     3481 gccaagcccg cttacgccgc attgatcaag ttcaaaaata agttccggtt ggtgaatgtc
     3541 ctgaggcaat ggacttattc agacaagaat tccgaggatt acattcttga gcagatcaga
     3601 ggaaagtacc cgaaggctga gatggtgctt tcacatatac cttacggaca gttggtcaag
     3661 aaagacgggg ctttggtgac tatatcaagc gccactgaac tccataattt cgaacaactt
     3721 tggctgccgt tggctgacta caaattgatt aacactttgc tcaagaccaa ggaggataac
     3781 ctggtcgaca tccttcataa ccgcctggat ctgcccgaga tgaccatcga gtccgccttt
     3841 tataaagcat ttgacagtat cctgtccttt gctttcaaca ggtatgccct gcatcagaac
     3901 gcgcttgtga agctgcaggc tcatcgggac gacttcaatg ctctgaatta cgaggacaaa
     3961 cagcagaccc tggaacgaat actggatgcc ctgcacgcat cccccgcctc tagcgacctt
     4021 aaaaaaatca atctctcttc cggcttcggt cggctgttta gcccaagtca cttcaccctg
     4081 gccgacacgg atgagttcat tttccagtct gtgaccggac ttttcagcac tcagaaaact
     4141 gtggcacagc tttaccagga aaccaaa
//
