LOCUS       sequence-195263-        4494 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-H1DEI0
FEATURES             Location/Qualifiers
     source          1..4494
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaaacta cgctgggtat cgaccttggg accaatagta ttggactggc actggtcgac
       61 caagaggagc atcagattct gtactccgga gtaagaatct tccccgaagg gatcaataaa
      121 gatacaatag ggctggggga gaaggaggag agccggaacg caacaaggcg cgccaaacgg
      181 caaatgaggc gacagtactt tagaaagaag ctgagaaagg ccaagttgct ggaactgctc
      241 atcgcctacg atatgtgtcc tctcaagccc gaggacgtaa ggagatggaa gaactgggat
      301 aaacagcaga aatccactgt gagacagttt cctgacacac ctgcttttag ggaatggttg
      361 aagcagaacc cgtacgaact gaggaagcag gccgtcacgg aagatgtgac cagaccggag
      421 ctcggtcgca ttctttacca gatgattcaa cgccgcggct ttctgagttc acgcaaggga
      481 aaggaagaag ggaaaatctt cacggggaaa gatcgaatgg tgggaatcga tgaaacaagg
      541 aaaaacctcc agaagcagac gctgggagcc tacctctatg atattgctcc caagaatgga
      601 gagaaataca gattccgcac tgaaagggtc agagcacggt atacgcttcg cgacatgtac
      661 atccgcgagt tcgaaatcat ctggcaaaga caagcaggtc accttggcct tgcccacgag
      721 caggccacca ggaagaagaa tattttcctg gaggggtctg ccactaatgt tcgcaactcc
      781 aagttgatta cccatctgca ggcaaaatac ggtcgggggc atgtcttgat tgaagatacc
      841 agaatcaccg tgacgtttca gctgcccctt aaggaagttc tcgggggcaa aattgaaatc
      901 gaagaagaac agcttaaatt caagtctaat gaatctgtgt tgttctggca gcgtccactc
      961 cggagtcaga aatctcttct gtctaagtgt gtatttgagg gaaggaattt ttacgaccca
     1021 gttcatcaga agtggattat cgccggtcca acgcccgccc ctctgagcca cccagagttt
     1081 gaggaattta gggcgtacca gttcatcaat aacattatct acggaaagaa cgagcatctt
     1141 acagcaatcc agcgcgaggc tgtattcgaa ttgatgtgta ctgagtccaa agacttcaat
     1201 tttgagaaaa tcccgaagca cctgaagctc ttcgagaagt ttaattttga cgacaccaca
     1261 aaggtgcctg cctgcaccac aatctcacag cttcggaaac ttttcccaca ccccgtgtgg
     1321 gaggagaagc gggaggagat ctggcattgc ttctacttct acgatgataa cactctcctc
     1381 tttgaaaaac tccagaagga ctatgctctc caaaccaacg atctggagaa aatcaagaag
     1441 ataagactgt ccgaatccta cggaaatgtc agcctgaaag ccattcggag gattaatcca
     1501 tatctgaaga aaggatacgc ttatagtacc gccgtcctgc tcgggggtat ccggaattct
     1561 tttgggaaga gatttgaata tttcaaagag tacgaacctg agattgaaaa agctgtctgt
     1621 aggatcctga aggagaaaaa tgcggagggt gaagtgatcc gcaagatcaa agactatctg
     1681 gtgcacaata gatttgggtt tgccaaaaat gatcgggctt tccagaaact gtaccaccat
     1741 tcccaagcca tcaccactca ggcacagaaa gagcgcttgc ccgaaaccgg caatctgcgg
     1801 aaccccatcg ttcagcaggg tctgaacgag ctgcggagga ccgttaacaa gctgctggcg
     1861 acctgtcggg agaagtacgg cccaagtttc aaatttgacc acatccacgt ggaaatgggc
     1921 cgggagctgc ggagtagcaa aaccgagcga gagaagcaga gcaggcagat tagagagaat
     1981 gaaaagaaaa acgaggccgc caaagtcaag ctcgctgagt acggtttgaa ggcctaccga
     2041 gacaacatcc agaaatatct tctgtataaa gagatcgagg agaagggtgg caccgtctgt
     2101 tgcccttaca ccggtaaaac tctgaatatc tcccacactt tgggctcaga taacagtgtg
     2161 cagatagaac atattattcc ttatagcatt agcctggatg atagtctcgc taacaagacc
     2221 ctgtgcgacg ccacctttaa tagagagaag ggcgaactca ccccttacga tttttaccag
     2281 aaggacccta gtcccgagaa atggggggcg tctagctggg aggagattga agaccgggct
     2341 ttcaggctcc tgccatatgc taaagctcag aggtttatta ggcggaagcc ccaggagtca
     2401 aacgaattta tctcccggca gctgaatgac acccggtaca tctcaaaaaa ggcagttgag
     2461 tatcttagtg ccatctgcag cgatgtaaag gcttttcctg gccaactgac agccgaactg
     2521 agacacctgt ggggtctgaa caacatcctg cagagcgcac ctgatattac cttcccactg
     2581 cctgtgtctg ccaccgagaa ccaccgcgag tattatgtta tcaccaacga gcaaaatgag
     2641 gtgattcgac tgttcccaaa acagggagaa actccaagga cggagaaagg ggagcttctc
     2701 ctgacaggag aggtggagcg gaaagtgttt cggtgtaagg ggatgcagga gtttcagaca
     2761 gatgtgtccg atggcaagta ttggagacgg atcaagctct ctagcagcgt cacctggtcc
     2821 cccctgttcg cccctaaacc aatcagcgct gatgggcaga ttgtattgaa gggtcgcatt
     2881 gagaaaggcg tctttgtatg taatcagctg aaacagaagc tcaaaacagg cttgcctgac
     2941 ggatcctact ggatttccct ccctgtgatc agccaaacct tcaaggaggg tgagagcgta
     3001 aataattcca aactgactag ccagcaggtg cagttgtttg gacgcgtgag ggaaggaatt
     3061 ttccgatgtc acaactatca gtgcccggca agtggtgctg atggcaactt ctggtgtact
     3121 ttggatactg acacagccca acctgccttc acgccaatca aaaacgcacc tccaggggtc
     3181 ggcgggggcc agatcatcct taccggcgat gttgacgata agggaatatt tcatgccgac
     3241 gatgacctgc attatgagct gcctgccagc ctccccaaag ggaaatacta cgggatcttc
     3301 actgtcgagt cctgtgaccc tacacttatc ccaatcgaac tgtcagcccc aaaaacttcc
     3361 aaaggcgaga atctcattga aggcaacatc tgggtggacg agcatactgg agaggtcagg
     3421 tttgacccaa aaaaaaatcg agaggatcag cgccaccatg ccatcgacgc tattgttata
     3481 gctctctcta gtcagagcct tttccagaga ctgagcactt ataatgccag gagagagaac
     3541 aaaaaaaggg gacttgactc caccgagcat tttccaagcc cgtggcctgg attcgctcag
     3601 gacgtgagac agagtgttgt gcccctcttg gtttcctata aacagaatcc gaagactctg
     3661 tgcaagattt caaagaccct ctataaggat gggaagaaaa ttcacagctg cggtaacgcc
     3721 gtgaggggcc agctccataa ggaaactgtg tacggtcaga ggaccgcgcc cggcgcaacc
     3781 gagaaatctt accatattcg aaaagatatc agggaactga agactagtaa gcacatcggg
     3841 aaggttgtcg acataaccat ccggcaaatg ctgctgaagc atctgcagga gaattatcat
     3901 attgatatca ctcaagaatt taacatccca agcaacgcct ttttcaagga gggagtttac
     3961 aggatcttcc tccctaataa gcacggcgaa ccagtgccca tcaagaaaat ccgcatgaaa
     4021 gaagagctgg gaaacgctga gagactgaag gataatataa accagtacgt caatcccagg
     4081 aataatcacc atgtcatgat ctaccaggac gccgatggca acttgaaaga agaaattgtc
     4141 agcttctgga gcgtgatcga gcgccagaac caaggccagc ccatttatca gctcccccgc
     4201 gaagggagga atatcgtgag tattctccag attaacgaca cttttcttat cggactgaag
     4261 gaggaggagc ctgaggtgta cagaaatgat ctgtcaaccc tgagcaaaca tctgtataga
     4321 gtgcagaaac tgtccggcat gtattatacg ttccggcacc acctggcctc tacacttaat
     4381 aatgaaagag aggaattcag gattcaaagt cttgaggcct ggaagagagc caaccctgtc
     4441 aaggtgcaga ttgacgagat tggccggatt acctttctga atggtccgtt gtgt
//
