LOCUS       sequence-195265-        3186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-I0X7Z6
FEATURES             Location/Qualifiers
     source          1..3186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgattatga aactggagaa gtggaggctc gggttggacc tgggaaccaa tagtatcgga
       61 tggtctgtgt tttccttgga taaggataat tccgtgcagg acttgattga tatgggtgtt
      121 cgaatattca gtgatggccg ggaccctaag acaaaagaac cgctcgctgt tgctagacgg
      181 acggcacgct cccagcgcaa actgatctac aggcgaaagc tgcgccggaa acaggttttc
      241 aagttcctcc aggagcaggg actgttcccc aagactaaag aggaatgcat gacattgaaa
      301 tccctgaatc cgtatgaact gcgcatcaag gccctggacg aaaaactgga gccgtacgag
      361 ctcggtcggg cactctttaa tttggctgtt agaagggggt tcaaatctaa ccgcaaggac
      421 ggaagccgcg aggaggtaag tgaaaaaaaa agcccggatg agattaaaac ccaggccgat
      481 atgcagaccc atctggagaa agcgatcaag gagaacgggt gccgaacaat caccgagttt
      541 ctgtacaaga accaggggga gaacggaggc atacggttcg cgccaggcag gatgacgtac
      601 taccctacca gaaaaatgta cgaggaggag tttaatctca tccggagtaa acaagagaaa
      661 tactaccccc aggtcgactg ggatgatatc tataaggcaa tcttctacca aaggcccctg
      721 aagccccagc agaggggata ctgcatctat gaaaacgaca aagagcgaac atttaaggcc
      781 atgccctgca gtcagaagct gaggatcctc caggacatcg gcaatctcgc ttattacgaa
      841 ggggggtcca agaagagggt tgagctgaac gataaccaag ataaagtgtt gtacgagctc
      901 ctcaatagca aggataaggt gactttcgac cagatgcgca aggccttgtg tctggcggat
      961 agcaactcat tcaacctgga ggagaaccgc gacttcctca tcggcaatcc tacagccgtg
     1021 aaaatgcgaa gcaagaatcg ctttggtaag ctctgggacg aaattccttt ggaagaacaa
     1081 gatcttataa tcgagactat aattactgca gatgaggacg acgctgtcta cgaagtaata
     1141 aaaaagtacg acctcacaca ggagcagagg gattttatcg tgaaaaacac tatactgcag
     1201 tccggaacta gcatgctctg caaagaggtt tctgaaaagt tggtgaaaag actggaggag
     1261 atcgccgacc tgaaatatca cgaggctgtg gagtctttgg ggtacaagtt cgccgaccag
     1321 accgtcgaga agtacgacct gctgccttac tatggaaagg tgctccccgg ctccacaatg
     1381 gagattgatc ttagtgcccc tgaaacgaat cccgagaagc actatggcaa aatatcaaat
     1441 ccaaccgtgc acgtggcgct caatcaaaca cgagtcgttg tgaacgctct tatcaaggag
     1501 tacggaaaac ccagccagat tgcaattgag ctgagtcggg accttaagaa taacgtcgag
     1561 aagaaagctg aaatcgccag gaagcagaac cagagagcca aggagaacat cgccatcaat
     1621 gataccattt ccgccctgta ccacacagct ttcccaggga aaagttttta cccaaaccgg
     1681 aacgatcgaa tgaagtaccg gctttggtca gaactgggcc tcgggaacaa gtgtatttat
     1741 tgtgggaaag gcatttccgg agctgagctg tttactaaag agattgagat tgaacacatt
     1801 ctgccgttct cccggactct gctggatgct gagagtaatc tcacagtggc tcactccagc
     1861 tgcaatgctt tcaaggctga gcgatccccc ttcgaggcgt tcggaactaa ccccagcggc
     1921 tactcatggc aagagatcat acagcgcgct aaccagctga aaaacacctc caaaaagaac
     1981 aagtttagcc caaatgccat ggatagtttt gaaaaggact caagctttat cgcccgccag
     2041 ttgagtgaca atcagtatat agcaaaggcc gctctgcgat acctcaagtg cctcgtggaa
     2101 aacccatccg acgtgtggac aaccaacggt tccatgacca aacttcttag agataagtgg
     2161 gagatggaca gcattctttg ccggaaattc acggagaagg aagttgccct cctgggcctg
     2221 aaacccgaac aaatagggaa ttataagaaa aacaggtttg atcatcggca tcacgccatt
     2281 gatgccgtag ttattggact gacagacaga tccatggtgc aaaagctggc aaccaagaac
     2341 agccataagg gcaacagaat cgagatcccg gagtttccga tcctgcggtc tgacctcatt
     2401 gagaaggtta agaacattgt agtatccttt aaacccgatc acggggctga gggcaagctg
     2461 tctaaagaaa cactgctggg aaagataaag ctccacggga aagaaacttt cgtttgtcgc
     2521 gaaaatatcg tgtctttgtc cgagaagaat cttgatgaca tcgttgatga gaagattaaa
     2581 tctaaggtca aagactacgt ggccaagcac aagggacaaa agatagaagc tgtgctgagt
     2641 gatttctcaa aggaaaacgg aattaagaaa gtaagatgtg taaatcgggt acagacgcca
     2701 attgaaatta cctcaggtaa aatctctcgc tatctgtccc ccgaggacta cttcgcagca
     2761 gtcatttggg agatcccagg ggaaaaaaaa accttcaaag cacagtacat tcggagaaac
     2821 gaggtggaga agaattctaa gggattgaat gtggtcaaac cagccgtcct cgagaatggg
     2881 aagccacatc ccgccgctaa gcaagtgtgt ctgctccaca aagacgatta cctggagttc
     2941 agcgataaag gcaagatgta cttctgcagg atcgccggat atgcagccac taataacaag
     3001 ttggatatta gacccgtgta cgcagttagc tattgtgcag attggataaa ttctactaac
     3061 gaaaccatgt tgaccgggta ctggaagcct acacccactc agaattgggt gtccgtcaac
     3121 gttctgttcg acaagcagaa ggcacgactc gtgactgtat ctcctatcgg tcgggtcttt
     3181 cgaaag
//
