LOCUS       sequence-195267-        3699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-I0ZZR5
FEATURES             Location/Qualifiers
     source          1..3699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaaaaga aaagaaaagt gacgttggga ttcgacctgg gcatagcatc tgtgggatgg
       61 gccattgtgg attctgaaac caaccaggtt tacaaactgg gttccagact gttcgacgcc
      121 cccgatacca atctggagag gcggacccag aggggcaccc gcaggctgct ccgaaggagg
      181 aaatacagaa atcagaagtt ctataatttg gtcaaaagga cggaggtctt cggattgtca
      241 tcccgggagg ccattgagaa ccggtttaga gagttgtcta taaagtatcc taatatcata
      301 gagctgaaaa cgaaggccct ctcccaggag gtgtgtcctg acgaaattgc atggatcctt
      361 cacgattatc tgaaaaatcg cgggtacttc tacgacgaga aggagactaa ggaagacttc
      421 gatcagcaga ccgtggagag catgccatct tacaagctga atgagtttta caagaagtac
      481 ggctacttta aaggcgccct gtcccaaccc accgagtccg aaatgaaaga taacaaggac
      541 ctgaaagaag catttttctt cgacttcagt aacaaagagt ggctcaaaga aatcaactat
      601 ttcttcaacg tccagaaaaa catcctgagc gagacattta tcgaggagtt taagaaaata
      661 ttttccttta ctcgggatat ctcaaagggc cctgggagcg acaacatgcc ctctccctac
      721 ggaattttcg gcgaattcgg agataatggt caaggagggc ggtatgagca tatctgggac
      781 aagaacatcg gcaaatgctc cattttcacc aatgaacaga gagccccaaa gtatctgccc
      841 tccgcactca ttttcaactt tctgaatgag ctggccaata tccgcctgta ctccactgac
      901 aagaagaata tccagccatt gtggaagctg tcctcagtag acaagttgaa tattcttctc
      961 aatttgttta acctgcctat tagtgagaag aaaaagaaac ttacctctac aaatattaat
     1021 gacatagtga agaaagaatc tattaaatct attatgatct ccgtagagga catcgatatg
     1081 attaaggacg aatgggctgg gaaggaaccc aatgtgtacg gagtgggtct gtctggattg
     1141 aacatcgaag agtccgccaa ggaaaacaag ttcaagttcc aggatcttaa gattctgaat
     1201 gttttgatca atctgctgga caacgtgggg atcaagttcg aatttaaaga cagaaacgat
     1261 attatcaaga atctggagct gctcgataat ctgtacctct tcctgatcta ccaaaaggaa
     1321 tctaacaaca aagactcatc tattgatctc tttattgcta aaaacgaatc tcttaacatc
     1381 gagaacctga aactgaaact gaaggagttc ttgctgggtg ctgggaacga gttcgaaaat
     1441 cataattcca agacacacag tttgagtaaa aaagctatcg acgagatctt gccaaaacta
     1501 ctcgacaaca atgaggggtg gaacctggaa gctatcaaga attacgatga ggaaatcaaa
     1561 tcacagatag aggacaattc ttctctgatg gccaagcagg ataaaaagta tctcaacgac
     1621 aattttttga aagatgctat cctgccccca aatgtaaagg taacttttca gcaggccatc
     1681 ctcatcttca ataaaatcat acagaagttt tctaaagact tcgagattga taaagtggtg
     1741 atagagcttg ctcgggagat gacccaggac caggaaaacg atgcgcttaa aggcatagcc
     1801 aaggcgcaga aatccaaaaa gagcctggtg gaggagcggc tggaggcaaa caacattgac
     1861 aaatcagtct tcaacgacaa gtatgagaag ctcatctaca agatctttct ttggattagc
     1921 caggatttca aagacccata tacaggcgct cagatatctg tgaacgagat tgtcaacaac
     1981 aaggtggaga ttgatcacat catcccatat tctttgtgct tcgacgactc tagtgcaaat
     2041 aaggtactcg tccataagca gtccaaccag gagaaatcta attctctccc ttatgaatac
     2101 ataaagcaag gacatagtgg ctggaactgg gatgagttca ctaagtacgt aaagcgggtt
     2161 tttgttaaca acgtggacag tattcttagt aaaaaagaga ggttgaaaaa atccgagaat
     2221 ctgctgactg cgtcctacga cgggtatgac aagctgggct tcctcgcacg caacttgaac
     2281 gacacacgct acgcaacgat tttgtttcga gatcagctga acaactacgc cgagcaccat
     2341 ttgattgaca acaagaagat gttcaaagtg atcgcaatga atggggctgt gacaagtttc
     2401 atacgaaaga atatgtcata cgacaacaaa ctgcgcctga aggacaggtc cgacttcagc
     2461 caccacgctt acgacgccgc aatcattgca ctctttagta ataagaccaa gacactgtac
     2521 aacctgattg atccctccct gaatggtatc atatccaaaa ggagcgaagg atactgggtc
     2581 attgaagacc ggtacaccgg ggaaattaaa gaactgaaaa aggaagactg gactagcatc
     2641 aaaaataacg tccaggccag gaagatcgca aaggagatcg aagagtacct tattgacctg
     2701 gacgacgaag tgttcttttc tagaaaaaca aaaaggaaga ccaatcgaca gctgtataac
     2761 gagacaattt acggaatcgc aaccaagacc gacgaagacg gtattacaaa ctattacaaa
     2821 aaggagaaat tctccattct ggatgacaaa gatatctatc ttagactcct ccgcgaaagg
     2881 gagaaatttg tgataaatca aagcaacccc gaagtgatcg accagataat tgaaatcatt
     2941 gagagctacg gcaaggagaa taacatcccc agtagggatg aggccatcaa cattaagtat
     3001 accaaaaaca agataaacta caacctgtat ctgaaacaat acatgcgatc tctcaccaag
     3061 tctctggacc agttcagtga ggagtttatc aaccagatga tcgctaacaa gacatttgtt
     3121 ctctacaacc ctacaaagaa cactacaaga aagataaagt tcctgagatt ggtgaatgac
     3181 gtgaagatca atgacattag gaagaaccaa gtaattaaca agtttaatgg caagaataat
     3241 gaaccaaaag ccttttatga aaatatcaac tctcttggcg caatcgtctt taaaaattct
     3301 gcgaataatt ttaagacgct ctctatcaat acacagatag ctatctttgg agacaagaat
     3361 tgggacatag aggatttcaa gacatataat atggagaaga tcgaaaagta taaggagata
     3421 tatggcatcg ataaaacgta caacttccac agtttcattt ttcccgggac cattctgttg
     3481 gataaacaaa ataaagaatt ttattatata tcatccattc agaccgtgcg agacattatt
     3541 gagatcaagt ttctcaacaa gattgagttt aaagatgaga acaagaatca ggatacatcc
     3601 aaaaccccaa agagattgat gtttggtatc aaatctatta tgaacaacta tgagcaagtt
     3661 gacatcagcc cgttcggcat taataagaaa atttttgaa
//
