LOCUS       sequence-195269-        3591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-J5TNY4
FEATURES             Location/Qualifiers
     source          1..3591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctcatgt ccaagcacgt gctggggttg gatctggggg tcgggtcaat tggctggtgc
       61 ttgattgccc tggacgctca gggtgatccc gctgaaattc tgggcatggg gagccgcgtc
      121 gtgcccctga ataatgctac aaaagcaatt gaggctttca atgccggagc tgccttcact
      181 gcaagccaag agcgaacagc aagaagaacg atgagacgcg ggttcgcccg ctatcagttg
      241 cgcagatacc gcctgagaag ggaacttgag aaagttggga tgttgcccga tgctgctctg
      301 atccaactgc ccctcctgga actgtgggag cttcgggaaa gagccgcgac cgcaggccga
      361 cggctgaccc tccctgagct gggacgcgtg ctgtgccaca tcaatcagaa gcgcggctac
      421 agacatgtaa aaagcgatgc tgctgccatt gtgggcgacg agggcgagaa aaagaaggac
      481 tctaattctg cgtatctggc cggaattagg gcaaatgatg agaagctcca agctgaacac
      541 aagactgtcg gacagtactt cgccgaacag ctgcggcaaa atcagagtga aagtcctact
      601 ggaggcataa gctacagaat taaggaccag atcttctctc gccaatgtta catcgacgag
      661 tacgaccaga tcatggctgt gcagagagtg cattatcccg acattctgac ggacgagttt
      721 atcagaatgc tgagggacga ggtaatcttc atgcaacgcc ccctcaaatc ttgtaagcac
      781 ctggtgtctc tgtgcgaatt tgagaagcaa gagcgcgtca tgcgggtcca gcaggacgac
      841 ggcaagggag gctggcagct ggtggaacgg agggtcaaat ttgggcccaa agtggccccg
      901 aaatcctcac cactgtttca actgtgctgt atctacgaag ccgtgaataa catccggctc
      961 acacgcccaa acggatcccc atgtgacatt acacctgagg aacgggctaa gatcgtggcc
     1021 catctccaga gtagcgcttc tctttccttt gccgctttga agaagctgct caaagaaaaa
     1081 gcgctgattg cagaccagct cacaagcaag tccggtctga agggtaactc cactcgcgtg
     1141 gcacttgctt ctgctcttca gccctacccc caataccatc acctgcttga catggagctt
     1201 gaaaccagaa tgatgaccgt ccagttgacc gacgaggaaa ccggggaagt taccgagcgc
     1261 gaggtcgccg tcgtaactga ctcatacgtg cgaaagcctc tttatcgcct gtggcatata
     1321 ctgtacagca tcgaggagcg ggaggccatg cggagagcgt tgatcacaca gctggggatg
     1381 aaagaggagg atctggacgg aggcctgctc gaccagctgt accggctgga cttcgtcaag
     1441 cctggctacg ggaacaagtc tgctaagttc atttgtaagc tgctccctca gctccagcag
     1501 ggactgggct actctgaagc atgcgccgct gtcgggtata gacattccaa tagtccaact
     1561 agtgaggaga tcaccgagcg gactctgctg gagaaaatcc ctctgctgca gcggaacgaa
     1621 ctgcgccaac ccttggttga gaaaattctg aatcagatga tcaatttggt caacgctctg
     1681 aaggcagagt atggtatcga cgaggtccgc gtggagctgg caagagaact gaaaatgtcc
     1741 cgggaggaac gcgagagaat ggcacggaac aataaggacc gggaggagcg aaataaaggc
     1801 gtggctgcca agatccggga gtgtggtctt taccccacaa agcccagaat ccagaaatac
     1861 atgctgtgga aggaagccgg taggcagtgt ctctactgcg gccgatcaat tgaagaggag
     1921 cagtgtcttc gagagggtgg aatggaggta gagcatatta ttcctaagtc agtcctgtat
     1981 gacgattcat acggaaataa gacctgtgca tgcaggagat gcaacaagga gaagggcaac
     2041 cggaccgctc tggaatacat tagagctaag gggcgcgagg ccgagtatat gaaacgcatt
     2101 aacgacctgc tcaaagagaa gaagatctca tacagtaagc accagagact tcgctggttg
     2161 aaggaggaca tccccagcga cttcctcgag cgacaactgc ggcttaccca gtatataagt
     2221 cgccaggcaa tggccatttt gcaacaggga atccgcagag tgagtgcttc cgaaggggga
     2281 gtcacagcta gattgaggtc cctgtggggg tatggcaaga ttctccacac actcaacctt
     2341 gataggtatg atagtatggg cgaaacagaa cgcgtttctc gagagggcga ggccaccgag
     2401 gaacttcata tcacgaactg gtccaagaga atggatcacc ggcatcatgc aatcgacgct
     2461 ctcgtcgtcg cctgtacccg ccagtcttat attcagcggc tcaatcggct tagtagtgag
     2521 ttcggcaggg aggataagaa gaaagaagat caggaggctc aagaacaaca ggccaccgaa
     2581 accggccggc ttagtaacct cgaacgctgg cttacccagc gtccccactt ttctgtgaga
     2641 acggtgtctg acaaggtcgc ggaaattttg attagttacc gaccaggcca gcgggtagtg
     2701 actagaggga gaaacatcta ccgcaagaag atggctgacg ggagggaagt gagctgcgtc
     2761 caaaggggtg tcttggtacc tcggggagag ctcatggagg cgagctttta tgggaagatt
     2821 ctctcccagg ggagggtgcg catcgtgaag agatacccac tccatgacct gaaaggtgag
     2881 gtagtggatc cacatctgcg cgaactgatc accacatata atcaggaact caaaagccga
     2941 gaaaaagggg ctcccatccc ccctctgtgt ctggacaaag ataagaagca agaggtccga
     3001 agcgtgaggt gttacgctaa aactctgagc ttggacaagg caattcccat gtgctttgac
     3061 gaaaaaggcg aaccgactgc gttcgtcaaa agtgccagta atcaccacct cgctttgtat
     3121 cggacaccca aggggaaact ggtggaatcc atcgtcacat tttgggatgc ggtcgaccga
     3181 gctaggtacg ggatccctct ggtgattacc cacccaagag aggtcatgga acaagtgctt
     3241 cagcgcgggg acatacctga acaggtattg agtctgcttc ccccttccga ctgggttttt
     3301 gtggattcac tccagcagga cgaaatggtg gtcatcggct tgagcgacga ggaactgcag
     3361 cgggctttgg aagcgcaaaa ttaccgcaaa atctccgagc acctctatcg ggtgcagaaa
     3421 atgtcctcaa gttactatgt gtttcgatat caccttgaga catcagtggc tgatgacaag
     3481 aatacttccg gacgaatccc aaagttccac cgcgttcagt ctctgaaagc ctacgaggaa
     3541 cgaaatatta ggaaagtccg ggtcgacctg ctgggacgaa tctccctgtt g
//
