LOCUS       sequence-195271-        3177 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-J6LE60
FEATURES             Location/Qualifiers
     source          1..3177
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggaatcc gcttcgcctt tgatctcggc accaacagca tcggctgggc cgtctggagg
       61 actggccctg gcgtttttgg agaggacacc gcggctagcc tggacggttc aggggtcttg
      121 atctttaagg acggccgcaa ccccaaggac ggccagtcac tggccactat gaggcgcgtc
      181 ccccggcaga gccgcaagcg aagagatcgg tttgtgctcc ggcgccggga cctcctggcc
      241 gccctgcgca aggctggtct gttcccagtg gacgtcgagg agggaaggcg actcgcagct
      301 acggacccgt atcaccttcg cgctaaagcg ctggacgagt cattgacacc acacgagatg
      361 gggagagtga tctttcacct gaaccagaga cgcgggttta ggagtaatag aaaagctgac
      421 agacaagata gagagaaggg caaaatagct gagggctcta aacgcttggc tgaaaccctc
      481 gctgcaacta actgccggac tctgggggaa ttcctgtgga gcagacatcg cgggactcca
      541 cggacgcgga gcccgacacg cattaggatg gagggagagg gagccaaagc actctacgct
      601 ttctatccaa ctcgggagat ggttagagct gaatttgaga gattgtggac agctcaatct
      661 cgctttgcgc ctgatctgct cactccagag cggcacgagg agattgcggg gatcctgttt
      721 aggcagcggg atctggcccc tcctaaaatc gggtgctgca cctttgagcc tagcgaaagg
      781 aggctcccac gcgcccttcc cagcgtggag gccaggggaa tctacgaacg gctggctcat
      841 ctgcggatta ccaccgggcc tgtatccgac cggggtttga caaggcctga gcgggatgtg
      901 ctcgccagcg ccctgctggc aggcaagtcc ctgacattta aggcagtgag gaagacactt
      961 aagatcctcc cccacgcttt ggtgaatttc gaggaagccg gggagaaggg actcgacggc
     1021 gccctcactg ccaaactgtt gtccaagccg gatcattatg gagccgcttg gcacggtctg
     1081 tcattcgctg agaaggatac cttcgttggg aaactgcttg atgaagctga cgaagagcgg
     1141 ctgattagac gcttggtcac cgaaaaccgc ctgagtgagg acgccgctag aagatgtgcg
     1201 agtatccccc tggcggatgg gtacggccgg ttgggtcgga cagccaacac tgagattctg
     1261 gcagctctgg ttgaggagac agatgagact gggacagttg taacatacgc cgaagctgta
     1321 cgcagggctg gcgagaggac tggccgcaac tggcatcact ctgatgaaag ggacggagtc
     1381 atcctggacc ggctgcctta ttatggtgaa attcttcagc ggcacgttgt ccccggcagt
     1441 ggagagcccg aagagaagaa cgaggccgcc cgctggggac gccttgcaaa ccctactgtc
     1501 cacatcggcc tgaaccagct tcggaaagtc gtgaatagac tgatcgccgc tcatggaagg
     1561 cctgaccaga tcgtggtaga gctggcccgc gagctgaaac tgaaccgcga gcagaaggag
     1621 cgcttggatc gcgaaaatag aaagaacagg gaggaaaacg aacgcagaac agcaatcctg
     1681 gccgaacacg gacaacgcga taccgccgaa aataagatta gactgaggct gttcgaagaa
     1741 caagcgcggg ccaatgctgg catcgcactg tgcccctaca caggaagggc catcggaatt
     1801 gccgagctgt tcacttccga ggtggagatt gatcacattc tgcccgtaag cctgaccctg
     1861 gatgacagcc tcgctaatcg cgttctttgt cgaagggaag ccaatcgcga gaagcgacgg
     1921 cagaccccat tccaggcatt cggtgctacc ccagcatgga acgacatcgt cgcccgcgca
     1981 gctaagttgc ctcccaacaa gcggtggaga ttcgatccag ccgctctgga gaggttcgaa
     2041 agggaaggag gtttcctggg gcgccagctg aacgaaacaa agtatctgtc caggcttgct
     2101 aaaatttatt tgggaaagat ctgcgatccc gatcgcgtgt atgtgactcc gggcactctc
     2161 acgggcctcc tcagggcccg gtggggattg aattccatcc tcagtgacag taactttaaa
     2221 aaccggtcag atcatcgaca tcatgccgtt gatgccgttg tgattggagt ccttacgagg
     2281 ggtatgatcc agagaattgc acatgacgct gcccgcgccg aggatcaaga tctggacaga
     2341 gtgttccgcg acgtaccagt gcccttcgaa gactttagag atcatgtgcg agagagagtg
     2401 agcaccatca cggtggccgt gaagccagag cacggcaaag gcggcgctct ccatgaggat
     2461 acatcttacg gccttgtacc tgacaccgat cccaacgcgg cactgggcaa tctggtcgtg
     2521 cgcaaaccaa tacggtccct gacagctggc gaagtcgaca gggttcgaga tcgggcgttg
     2581 agggctcggc tgggagcctt ggctgcccca tttcgggacg agagtggaag ggtgcgcgac
     2641 gctaaggggc tcgcacaggc cttggaagct ttcggggccg agaatggtat ccggcgggta
     2701 cggattctga agcctgacgc tagtgttgtg actatcgccg atcgccgcac cggagtacca
     2761 tatcgcgcag tcgctccagg ggagaatcat cacgtggaca ttgtgcaaat gcgggacggt
     2821 agttggcgag gcttcgctgc cagtgtgttt gaggtaaata ggcccggatg gcgcccagaa
     2881 tgggaggtga agaagctggg cggcaaactc gtgatgcgcc tgcacaaggg ggacatggtt
     2941 gagctgtccg acaaagacgg tcagcgcagg gtcaaggtcg tgcagcagat tgaaatttca
     3001 gctaacaggg tgagattgag ccctcataat gatgggggga agctgcaaga cagacacgcc
     3061 gatgccgatg acccctttcg gtgggatctg gccacaattc cactgcttaa agacagaggt
     3121 tgtgtggccg tccgcgtgga tcccatcggt gtcgttaccc tgaggagaag caacgtg
//
