LOCUS       sequence-195273-        3459 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-K0XCK7
FEATURES             Location/Qualifiers
     source          1..3459
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaaaaaca tcttggggct ggacctgggc ttgtcctcta tagggtggtc cgtgatacgc
       61 gagaattctg aagaacagga gctcgtggct atgggttcaa gggtggtatc cctgaccgcg
      121 gccgaacttt cttcctttac tcaaggaaat ggagtcagca tcaattctca gcgaactcaa
      181 aaaagaactc aacggaaggg ttacgaccgg tatcagctta ggaggactct gctgagaaac
      241 aagcttgata ccctggggat gttgcccgac gatagtctgt catacctgcc aaagctccag
      301 ctgtgggggc tgagggcgaa ggctgtaaca cagcgcattg aactcaacga gctcggaagg
      361 gtccttttgc atctcaatca gaagcgcggc tacaagagta taaaatccga cttcagcggt
      421 gataaaaaga tcacagacta cgtgaagacc gtcaaaacac gctatgatga actcaaggaa
      481 atgagactga ccatcggaga actctttttc agacgactta ccgagaacgc atttttccgg
      541 tgtaaggaac aggtctatcc ccgccaggct tacgtggaag agttcgactg tatcatgaat
      601 tgtcagagga agttttatcc cgatatcttg actgatgaga ctatccggtg cattagggat
      661 gagatcatct actatcagcg cccattgaag tcttgtaagt acctggtgag tcgctgcgaa
      721 tttgaaaaaa gattttacct gaacgccgcc ggtaagaaaa ctgaagccgg ccccaaggtg
      781 tctccccgga caagtccatt gtttcaggtg tgtcggctgt gggaatccat caataatatc
      841 gtggtgaagg acaggcggaa cgagatagtg tttatctccg ctgaacagag ggccgccctg
      901 tttgattttc tcaacaccca tgaaaagctt aagggttcag acctgcttaa attgctgggt
      961 ctttcaaaga cctatggtta cagactcgga gagcagttta agactgggat tcagggtaat
     1021 aagacaaggg tggagatcga gagagccctt ggcaactatc ccgacaagaa gcggctgttg
     1081 cagttcaacc tccaggaaga aagctcaagt atggtgaata cggaaaccgg tgaaatcatc
     1141 cccatgattt ccctcagctt tgaacaggaa cctctgtatc ggctttggca cgtcctttat
     1201 tcaattgatg acagagagca gttgcagtca gttttgagac agaagttcgg gatcgatgat
     1261 gacgaagttc tggaacgcct gagcgccatt gacctggtta aagccggctt cgggaataag
     1321 agtagcaagg ccattcggcg catcctcccc tttctgcaat tgggaatgaa ctatgcagag
     1381 gcttgcgaag ccgcagggta taatcacagc aacaactata ccaaagccga aaacgaggca
     1441 cgggccctgc tcgatagact tcctgctatc aaaaaaaacg agcttcgaca gcccgtggtg
     1501 gagaaaatac ttaaccaaat ggtgaacgtg gtgaacgcac ttatggagaa gtacgggagg
     1561 tttgatgaga ttagagtcga gttggctagg gaacttaaac agagtaaaga agaacgctca
     1621 aacacgtaca agagtattaa caagaaccag cgggaaaatg agcagatcgc taaaaggatc
     1681 gtggaatatg gggtgccaac taggtcacgg atccaaaagt ataaaatgtg ggaggagtct
     1741 aagcattgct gcatttattg cggtcagcct gttgatgtgg gggacttcct gcgggggttc
     1801 gatgttgagg tggagcacat cataccaaaa agcctctatt ttgacgacag ttttgcaaat
     1861 aaggtctgtt cctgcagaag ctgcaataaa gaaaagaaca atcggacggc ctacgactac
     1921 atgaagtcca agggggagaa ggcccttagc gactatgtcg agcgagttaa tacaatgtac
     1981 actaataacc agataagcaa gaccaagtgg caaaacttgc tgactccagt ggataaaatt
     2041 tcaatcgatt tcatcgaccg gcagcttcgg gaatctcagt acatcgctcg gaaagctaaa
     2101 gaaatcttga ccagtatttg ctacaacgtt accgccacca gcgggtccgt cacgagcttt
     2161 ctgagacacg tgtgggggtg ggataccgtg ctgcacgatc ttaattttga tcggtacaag
     2221 aaggtgggcc tcacagaagt cattgaagtg aaccatagag ggagtgtgat tagacgggaa
     2281 cagataaaag actggtctaa acgctttgac caccgacacc acgctattga tgctttgact
     2341 atagcttgta ctaaacaagc atacatccag cggctgaata accttcgcgc tgaggaagga
     2401 ccagacttta acaagatgtc cttggaacga tacatccaga gccagcccca cttttctgtg
     2461 gctcaggtgc gcgaggccgt ggacaggatt ctcgtgagct ttcgggcagg aaagcgcgct
     2521 gtcaccccag gaaagagata cataaggaaa aacaggaagc ggataagcgt gcagtccgtg
     2581 ctgatcccca gaggcgccct ctcagaagaa tccgtctatg gagtgatcca tgtctgggaa
     2641 aaggatgagc agggccatgt gatccaaaag caacgcgcag tgatgaaata ccccataaca
     2701 tcaattaaca gagaaatgct ggacaaggag aaggtcgtgg ataagagaat ccaccgcatc
     2761 cttagcggtc gactggccca gtacaatgat aatccaaagg aagcatttgc caaacccgtt
     2821 tacatcgaca aagaatgtcg cattcccatc cgcacggttc gatgcttcgc aaaaccagca
     2881 attaacaccc tggttcctct gaaaaaggac gacaagggta atcctgtggc gtgggtcaac
     2941 cctggtaata accaccatgt ggctatctac cgggacgagg atggcaagta taaagagaga
     3001 accgttacct tctgggaggc agtagataga tgtagagtag gaatccccgc tatcgtgacc
     3061 cagccagata caatttggga caatattctg cagcgcaacg acattagcga aaacgttctg
     3121 gaatccctcc ccgatgttaa gtggcagttt gtgttgagtc tgcagcagaa tgagatgttc
     3181 attctgggta tgaacgagga ggactataga tacgcaatgg atcagcagga ttacgctctg
     3241 ctgaacaaat atttgtacag agtacagaag cttagcaagt ccgattactc tttcaggtac
     3301 catacagaaa cgagcgtgga ggataagtat gacggaaaac ctaacctcaa acttagtatg
     3361 cagatgggga agcttaaaag ggtgagcata aaaagccttc tgggtcttaa ccctcacaaa
     3421 gttcacatct ccgtgctggg cgaaatcaaa gaaatctct
//
