LOCUS       sequence-195275-        4245 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-K1LM04
FEATURES             Location/Qualifiers
     source          1..4245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaaacata tcctggggct cgacctgggg actaacagta ttggatgggc tctcattgaa
       61 cgcaacatcg aggagaagta tggtaagatc atcgggatgg gctctagaat tgtgcctatg
      121 ggcgccgaac tctccaagtt cgagcaggga caagcacaga cgaaaaacgc cgatagacgg
      181 actaaccggg gagcccggcg gctgaacaag aggtacaagc aacgccgcaa taagcttata
      241 tacatcttgc agaaactgga catgctgccc tcccagatca aattgaagga ggatttctcc
      301 gaccctaata agatagacaa gattaccatt ctgccaatct caaaaaagca ggaacagctc
      361 accgctttcg accttgttag tctcagggtg aaagccctga ctgagaaggt cggactcgag
      421 gatctgggaa agataattta caaatataac caacttcgcg gttacgccgg aggctccttg
      481 gagcccgaaa aagaagatat attcgatgag gagcagtcta aagataaaaa aaacaaatcc
      541 ttcatagcct tttccaaaat agtgttcctg ggagaacctc aggaagaaat ttttaagaat
      601 aaaaagctca accgccgcgc catcattgtc gaaacggagg agggtaattt tgagggcagt
      661 acgtttctgg agaacattaa ggttggcgac tcactcgaat tgctgattaa tatctctgcc
      721 tctaagtccg gtgacacaat taccataaaa ctgcccaaca aaaccaattg gagaaagaaa
      781 atggagaaca tcgagaacca gctcaaagaa aagtccaaag aaatgggcag agagttctac
      841 atttcagagt tcctgttgga acttctgaag gagaacagat gggccaaaat ccgcaacaac
      901 actattctta gggcccgata cgagtccgag tttgaggcaa tatggaacga gcaggtgaag
      961 cactatccct tcctggagaa tctcgacaag aaaactctga tcgagatcgt gtcctttata
     1021 ttcccaggcg aaaaggagag tcaaaaaaaa tatagggaac tgggattgga gaaaggcttg
     1081 aagtatatca tcaaaaacca agtggtattc taccagcggg agcttaagga ccaaagccac
     1141 ctcattagtg attgccggta cgagccgaat gagaaagcga ttgctaagtc tcatcccgtg
     1201 tttcaggaat ataaagtgtg ggaacaaata aataagctga tcgtgaatac caaaattgag
     1261 gccggcacaa accggaaagg cgagaaaaag tacaaataca ttgatagacc tatcccaacc
     1321 gcccttaaag agtggatatt tgaggagctc cagaataaga aagagatcac cttttcagcc
     1381 attttcaaga aactcaaggc agagttcgat ctgcgagaag gtattgattt tctcaatggc
     1441 atgagcccta aggacaagct taaaggtaac gagactaaac tgcagttgca gaagtccctc
     1501 ggggaactgt gggatgtctt gggtctggac agcattaata gacagattga attgtggaat
     1561 attctctaca acgagaaggg caacgagtat gatctgactt cagatcggac tagcaaggtt
     1621 ctggagttta taaacaagta tggtaataat attgtcgacg ataacgctga agaaaccgca
     1681 attcgaatct caaagattaa gttcgcacgg gcctatagtt ccctcagcct caaagctgtc
     1741 gaacgaattc tgccattggt gcgggcggga aagtatttta ataacgattt ttcccagcag
     1801 ctgcaatcta aaattctgaa gctccttaat gagaacgtgg aggatccttt tgccaaggcc
     1861 gctcagacgt accttgataa caatcagtca gtgctgagcg agggaggtgt tggcaactcc
     1921 atcgccacaa tcctggtata tgacaagcac actgctaagg aatacagtca tgatgaactg
     1981 tataagtctt acaaggagat taacctcctc aagcagggcg atctcaggaa ccccctcgtg
     2041 gagcagatca ttaacgaagc cctcgtgttg atcagagata tctggaaaaa ctatgggata
     2101 aagccgaacg agattagggt cgagctggcc agagatctca agaattcagc taaggagaga
     2161 gccactattc acaaacggaa caaggataat cagactatca ataacaaaat caaggagact
     2221 ctcgtaaaaa ataagaaaga gctgtcactg gcaaatatcg agaaggtcaa actgtgggaa
     2281 gcccagcggc atctgtcccc ttatacaggg cagcccatcc ccctttctga tttgttcgac
     2341 aaagagaaat atgacgtaga ccacatcata cctatctcta ggtatttcga tgattcattt
     2401 accaataaag tgatatccga aaagtccgta aaccaggaga aagcgaatcg caccgctatg
     2461 gagtattttg aggtgggaag ccttaaatac agcattttta cgaaagagca attcatcgct
     2521 cacgtcaatg agtatttctc cggggtgaaa aggaaaaatc tcctcgcaac ttcaattcca
     2581 gaggatcccg tgcagcgaca gataaaggac acccaatata ttgcgattcg cgttaaagaa
     2641 gaactgaata agattgtggg caacgagaat gtcaagacaa ccactggttc aatcaccgac
     2701 tatcttcgga atcattgggg tctgactgat aagtttaagc tcctgctcaa ggaacgatac
     2761 gaggccttgc tggagagtga gaagtttctc gaggctgagt acgacaacta caagaaggac
     2821 ttcgactccc gcaaaaagga atatgaagaa aaggaggtgc tctttgaaga acaggagctg
     2881 acaagagaag aattcattaa ggaatataaa gagaactata tccggtataa aaagaacaaa
     2941 ctgattatta agggctggag caaacgaatt gatcatcggc accacgccat tgatgcgctc
     3001 atcgttgcat gcaccgaacc cgcgcacatc aagaggctca atgacctgaa taaggtcctg
     3061 caggattggc tcgttgagca caaatccgag ttcatgccca atttcgaggg atccaacagt
     3121 gagctccttg aagaaattct gagtttgcct gagaacgaaa gaaccgagat tttcacccag
     3181 atagagaagt tccgggccat agaaatgccg tggaaggggt ttccagagca ggtggaacag
     3241 aagctcaagg aaattatcat ttctcataaa cccaaagata agctgttgct tcagtacaat
     3301 aaggctgggg accgacaaat taaactgaga gggcagctgc acgaagggac cctctatggg
     3361 atttcccagg gtaaagaagc atatagaata cctctgacca agttcggcgg cagcaagttt
     3421 gcaacagaaa aaaacattca gaagatcgtg agtccttttc tgtcaggatt cattgcgaat
     3481 cacctgaaag agtataacaa caaaaaggag gaagcgttct ctgccgaggg aatcatggat
     3541 cttaacaaca agctcgctca gtaccggaat gaaaagggcg aactgaagcc tcatacccct
     3601 atttccaccg tgaagattta ttataaagac ccatcaaaaa ataaaaagaa aaaggatgag
     3661 gaggatctga gtctgcagaa gctggacaga gagaaagcct tcaatgaaaa actttacgtg
     3721 aagaccggcg acaactatct tttcgccgtg ctcgagggcg aaatcaaaac taaaaagact
     3781 tcacagataa agagacttta tgatatcatc tctttcttcg acgctacaaa ttttctgaag
     3841 gaggagttta ggaacgctcc agacaagaag acatttgata aagatctgct gttcaggcag
     3901 tacttcgagg aacggaataa ggccaaactg ctgttcactc tgaagcaggg agacttcgtt
     3961 tatcttccaa atgaaaatga ggaggtgatt ttggacaagg agtcacctct ctacaaccag
     4021 tactggggag atctcaaaga gaggggaaag aacatctacg tggttcagaa gttttccaag
     4081 aagcagattt attttattaa gcacacgatt gcagacatca ttaaaaagga cgttgagttc
     4141 gggtcccaga attgctatga aaccgttgag ggccgatcca tcaaggagaa ctgctttaag
     4201 ctcgagatcg accgcttggg caacattgtg aaggtgataa agaga
//
