LOCUS       sequence-195277-        2952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q0P897
FEATURES             Location/Qualifiers
     source          1..2952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..2952
                     /codon_start=1
                     /product="Cas9 endonuclease from the Campylobacter jejuni 
                     Type II CRISPR/Cas system (Kim et al., 2016)"
                     /label=CjCas9
                     /translation="MARILAFDIGISSIGWAFSENDELKDCGVRIFTKVENPKTGESLA
                     LPRRLARSARKRLARRKARLNHLKHLIANEFKLNYEDYQSFDESLAKAYKGSLISPYEL
                     RFRALNELLSKQDFARVILHIAKRRGYDDIKNSDDKEKGAILKAIKQNEEKLANYQSVG
                     EYLYKEYFQKFKENSKEFTNVRNKKESYERCIAQSFLKDELKLIFKKQREFGFSFSKKF
                     EEEVLSVAFYKRALKDFSHLVGNCSFFTDEKRAPKNSPLAFMFVALTRIINLLNNLKNT
                     EGILYTKDDLNALLNEVLKNGTLTYKQTKKLLGLSDDYEFKGEKGTYFIEFKKYKEFIK
                     ALGEHNLSQDDLNEIAKDITLIKDEIKLKKALAKYDLNQNQIDSLSKLEFKDHLNISFK
                     ALKLVTPLMLEGKKYDEACNELNLKVAINEDKKDFLPAFNETYYKDEVTNPVVLRAIKE
                     YRKVLNALLKKYGKVHKINIELAREVGKNHSQRAKIEKEQNENYKAKKDAELECEKLGL
                     KINSKNILKLRLFKEQKEFCAYSGEKIKISDLQDEKMLEIDHIYPYSRSFDDSYMNKVL
                     VFTKQNQEKLNQTPFEAFGNDSAKWQKIEVLAKNLPTKKQKRILDKNYKDKEQKNFKDR
                     NLNDTRYIARLVLNYTKDYLDFLPLSDDENTKLNDTQKGSKVHVEAKSGMLTSALRHTW
                     GFSAKDRNNHLHHAIDAVIIAYANNSIVKAFSDFKKEQESNSAELYAKKISELDYKNKR
                     KFFEPFSGFRQKVLDKIDEIFVSKPERKKPSGALHEETFRKEEEFYQSYGGKEGVLKAL
                     ELGKIRKVNGKIVKNGDMFRVDIFKHKKTNKFYAVPIYTMDFALKVLPNKAVARSKKGE
                     IKDWILMDENYEFCFSLYKDSLILIQTKDMQEPEFVYYNAFTSSTVSLIVSKHDNKFET
                     LSKNQKILFKNANEKEVIAKSIGIQNLKVFEKYIVSALGEVTKAEFRQREDFKK"
ORIGIN
        1 atggcaagaa tcttggcttt tgacattgga atcagctcta tcggttgggc gttttccgaa
       61 aatgatgagc tgaaagattg cggcgttcgg attttcacca aggtcgaaaa ccccaagacc
      121 ggggagtctc tcgctcttcc acgaagactc gccagatctg cccggaagcg cctcgctagg
      181 agaaaagcta gacttaacca cctgaagcat ctgattgcga atgaattcaa actgaactac
      241 gaggactacc agtcattcga tgaaagcctt gccaaggctt ataaggggag cttgatctcc
      301 ccctacgagc tgcgatttcg ggcgctgaat gagttgctga gcaaacagga cttcgcacgc
      361 gtcatactgc acatcgcaaa gagaagaggg tacgatgaca tcaagaacag cgacgataag
      421 gagaaaggag ccatccttaa agcgataaaa caaaacgaag aaaagcttgc aaactaccag
      481 agcgtgggag agtatctcta caaagagtat tttcagaaat ttaaggaaaa cagcaaggaa
      541 tttaccaacg ttcggaataa aaaggagagc tatgagagat gcattgctca gagttttctg
      601 aaagacgagt tgaagcttat atttaaaaag cagcgggaat ttggcttcag ctttagtaaa
      661 aaattcgagg aggaggtcct gtccgtggcc ttttataagc gggcgctcaa agatttctca
      721 catttggtgg ggaactgctc atttttcacc gacgaaaaga gagctcctaa gaactccccc
      781 ctggctttca tgttcgtcgc actcacgagg atcattaact tgctgaataa cttgaagaac
      841 actgagggca tactttacac aaaagatgat ctgaacgcct tgctcaatga agtccttaag
      901 aacgggacac tgacttataa gcaaacaaaa aaactcctgg gtctgtccga tgactatgag
      961 tttaaaggag aaaagggaac ctacttcatc gagttcaaga aatataagga atttataaaa
     1021 gcattggggg agcataactt gtcccaggat gatttgaatg agattgcaaa agacatcact
     1081 ctcattaagg atgaaatcaa gctgaaaaag gctctggcga aatacgacct caaccagaac
     1141 cagatcgatt cactgagtaa actggagttt aaggatcatc tgaatatctc cttcaaagca
     1201 ctcaaattgg tcacccccct gatgctggag ggcaagaaat acgacgaagc ttgtaacgag
     1261 ctgaatctta aggtagcaat taacgaggat aaaaaggact ttctgcccgc ctttaatgag
     1321 acgtattaca aagacgaggt cacgaaccct gtggtgctga gggcaatcaa agagtataga
     1381 aaggtactta atgccctcct taaaaagtat ggaaaagtgc ataaaattaa tattgagctg
     1441 gcccgcgagg tcggcaagaa ccactctcag agggcaaaga tcgagaaaga gcagaacgag
     1501 aactataaag ccaagaaaga tgcggagctc gagtgtgaga agctgggctt gaaaattaac
     1561 tctaagaata tcctgaaact gagactgttc aaagaacaga aggagttttg tgcttatagc
     1621 ggcgagaaga tcaaaatttc cgatctccag gatgagaaga tgctggagat tgatcatata
     1681 tacccttatt ccaggagttt cgacgacagc tatatgaata aagtgttggt gtttacgaag
     1741 cagaaccagg agaagttgaa tcaaacacct tttgaagcct ttggtaacga ttccgcaaag
     1801 tggcaaaaaa ttgaggtgct cgcaaagaac ctgcctacga aaaaacagaa gcgcatcctc
     1861 gataagaatt acaaagacaa ggaacagaag aatttcaaag accggaatct gaacgacacc
     1921 aggtacatcg ctagactggt gcttaattac actaaggact acctcgactt tctccctctg
     1981 tctgacgacg agaacaccaa acttaacgac actcaaaaag gatccaaggt gcatgttgag
     2041 gccaagagtg gcatgcttac aagtgctctg cgacacacat ggggtttcag cgccaaggac
     2101 aggaataatc atctccacca cgccatcgac gcggtcatta tcgcttacgc aaataacagt
     2161 atcgtcaagg ccttttcaga ttttaagaaa gagcaggaat caaattcagc cgagctgtac
     2221 gccaagaaaa tatctgagct tgactacaaa aacaagcgca agttttttga gccgttttcc
     2281 ggattcaggc agaaagtgct cgacaaaatt gacgagattt tcgtctcaaa acctgaaaga
     2341 aaaaagccat caggcgccct gcacgaggag acgttcagga aggaggagga attctaccag
     2401 tcttacggag gcaaggaagg ggtgcttaag gcactggagc tgggcaaaat ccgcaaggtt
     2461 aacggaaaaa tagtgaaaaa cggtgatatg ttccgcgtgg atatttttaa acacaaaaag
     2521 accaacaagt tttatgccgt cccaatttac acaatggact tcgctctcaa ggtgctcccg
     2581 aacaaagctg ttgcgagatc taagaaaggt gaaatcaagg actggatctt gatggacgaa
     2641 aattatgagt tctgtttcag cctgtataaa gactcactga tcttgatcca aaccaaggat
     2701 atgcaggagc ccgaattcgt ctactacaat gccttcactt ctagtaccgt ttccctcata
     2761 gtgtccaagc atgacaacaa atttgaaact ctgtccaaaa atcagaaaat actgttcaag
     2821 aatgccaatg agaaagaggt tattgcaaag tccatcggca tacaaaactt gaaggtgttc
     2881 gagaagtata tcgtgagcgc acttggcgaa gtcacgaaag ccgaatttag acagagggag
     2941 gacttcaaga aa
//
