LOCUS       sequence-195279-        3498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q1QGC9
FEATURES             Location/Qualifiers
     source          1..3498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1192..1203)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgcacgttg agattgattt cccgcacttc agcagaggtg acagccatct cgcaatgaac
       61 aagaatgaaa ttctgcgggg cagctctgtg ctctatagac tgggcctcga cctggggagt
      121 aattctctgg gctggtttgt tacacacctc gaaaagaggg gggacaggca tgaacccgtg
      181 gctctcggcc ctggtggtgt cagaattttc cccgatggga gagatccaca gtcaggtaca
      241 agtaatgcgg tggacaggag gatggcccga ggtgccagaa aaaggcgaga tcgatttgtc
      301 gaaagaagaa aagaattgat cgcggccctg attaagtata atctgctgcc ggacgatgct
      361 cgagaacgcc gggctctgga agtacttgat ccttacgcac tgcggaagac cgctctgact
      421 gacacacttc ccgcacacca tgttgggaga gctcttttcc atctcaatca gaggcgcggc
      481 ttccaaagca acagaaaaac cgacagcaag cagtctgagg acggtgccat taaacaggct
      541 gccagtcgcc ttgctaccga caaggggaac gagacgttgg gggtgttctt tgctgatatg
      601 cacctgcgaa aatcctatga agatcggcag actgccattc gcgcggagct ggttaggctg
      661 ggcaaagacc acctcacagg caacgctcgc aagaagatct gggcaaaggt caggaaaaga
      721 ttgtttggtg atgaggtctt gccacgggcg gacgctccgc acggcgttcg agccagagca
      781 accattactg gcaccaaagc cagctacgat tattacccca cacgcgatat gctccgagac
      841 gagttcaacg cgatctgggc cggtcaatct gcccaccatg ctactatcac tgacgaagct
      901 aggacagaaa ttgaacatat cattttttat cagaggcccc ttaagccggc aatcgtggga
      961 aagtgtacac ttgacccggc tactaggccc ttcaaggagg accccgaagg atatcgggcc
     1021 ccttggagcc accccctcgc ccagcgcttt cgcatcctga gtgaagccag aaatctggag
     1081 atcagggata ccggtaaagg cagcagacgc ctgaccaagg aacaatcaga ccttgtcgtc
     1141 gcagctctgc tggccaaccg ggaagtcaag ttcgataaac ttagaacact gctgaagctc
     1201 ccagccgaag ccagatttaa ccttgagagc gaccgccggg ccgcacttga tggagatcaa
     1261 actgccgcaa gactgagcga caagaagggg tttaataagg cctggcgcgg attcccaccc
     1321 gagcgccaaa tcgctattgt tgctagactc gaagagactg aggatgagaa cgagctgatt
     1381 gcctggctgg aaaaggagtg cgccctcgac ggcgcagctg ctgctcgcgt ggccaacacg
     1441 acactccccg atgggcactg caggctgggc ctcagggcga ttaaaaagat tgtgccaata
     1501 atgcaggacg gactcgacga ggatggggtg gcaggggcag gctaccatat cgccgccaag
     1561 agagcaggat atgatcacgc aaaacttccc actggagaac agctgggacg actgccttac
     1621 tacggccagt ggctccagga cgccgtggtg ggatccggcg atgcaagaga tcagaaggag
     1681 aaacagtacg ggcaatttcc taatcctact gtgcacattg gcctcggcca gctgcggaga
     1741 gtcgtgaacg atttgattga caagtatggc cccccaaccg agatctctat tgagtttaca
     1801 cgggcactga aattgtctga acagcaaaag gccgagcggc agcgggaaca gcggcgaaat
     1861 caggataaga acaaagcccg cgccgaagaa ctggcgaagt tcgggcgccc cgcaaaccca
     1921 agaaacctcc tgaaaatgcg actgtgggaa gaattggccc atgatccact ggatagaaag
     1981 tgcgtttata cgggggagca gataagcata gagcggctgc tttctgacga ggtcgacata
     2041 gatcacattc tccccgtggc catgacactg gacgactcac ctgcaaacaa gatcatttgc
     2101 atgcgatatg ctaaccgcca caagagaaag cagacaccct cagaggcctt tgggtcatca
     2161 ccgacacttc aggggcacag atataattgg gacgatatag ctgcccgggc aaccggtctg
     2221 ccacggaaca aacgctggcg gtttgacgca aacgccagag aggaattcga caagaggggc
     2281 ggatttctgg ccaggcaact gaacgagact gggtggctgg cccgcctggc aaagcaatac
     2341 cttggcgccg tgacggaccc gaatcagatc tgggttgtcc ctggtcggct cacctcaatg
     2401 ctgcgcggga agtggggcct taatggtctg cttcctagtg acaactatgc gggtgtgcag
     2461 gacaaggccg aagagttcct cgcatccacc gacgacatgg agttcagcgg cgtgaagaac
     2521 agggccgatc accggcacca cgcaattgac gggctggtga ctgccttgac cgacaggagt
     2581 ctcctctgga aaatggctaa tgcatacgac gaagagcatg aaaaatttgt gatcgagcca
     2641 ccttggccta ccatgaggga cgacctgaag gccgcgctgg aaaaaatggt cgtctctcat
     2701 aagccagatc acggaataga aggcaagctg cacgaggatt ccgcttacgg gttcgtgaaa
     2761 ccacttgacg ccaccggact caaggaggag gaggctggca acctggtgta tagaaaggct
     2821 atcgagtctc ttaacgagaa cgaggtggat cggatcaggg atatccagct tcggactatt
     2881 gtcagggatc acgtcaatgt agaaaagacc aagggtgttg ctctcgctga tgcactccgc
     2941 cagcttcagg ccccgagcga tgattaccct caatttaagc acggtctgcg gcacgtgagg
     3001 atattgaaaa aggagaaggg cgattatttg gtacccatag ctaaccgggc ttccggagtg
     3061 gcctacaagg cttactcagc gggagagaac ttctgcgtag aagtgtttga aaccgctgga
     3121 ggtaagtggg atggggaagc agtcaggcgc ttcgacgcca ataagaagaa cgccggcccc
     3181 aagattgctc acgctcctca gtggagagat gccaacgagg gggccaaact ggtaatgcgg
     3241 attcacaaag gggatctgat aaggttggac cacgaaggca gagcgagaat aatggttgtg
     3301 cacagacttg atgccgctgc ggggcgcttc aaactggctg atcataatga aaccggtaac
     3361 ctcgacaaga gacacgcaac aaacaacgac atcgacccct ttaggtggct gatggccagc
     3421 tacaataccc tgaagaagct ggccgcggtg cccgtgcgag tagatgaact gggcagagtg
     3481 tggcgggtca tgcccaac
//
