LOCUS       sequence-19528-e         554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF45
FEATURES             Location/Qualifiers
     source          1..554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(239..256)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    245..469
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctcctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cgggccctct agacccgggg agaagaaatt
      121 tgcctgccct gagtgtccta agcgcttcat ggatcgtagc cacctcgcac gtcatatcaa
      181 gacccacacc ggtggatcct ctgcacagta gtaagcttaa gtttaaaccg ctgatcagcc
      241 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
      301 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
      361 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
      421 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
      481 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
      541 gcggcgggtg tggt
//
