LOCUS       sequence-195281-        3519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q2RX87
FEATURES             Location/Qualifiers
     source          1..3519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaggccta ttgagccatg gattctcggt ctggatatcg gcacagactc tctggggtgg
       61 gccgtattca gctgtgaaga aaaggggccg cctactgcaa aggagctgtt gggcggaggg
      121 gtccgactct tcgatagcgg acgggatgcc aaggatcaca ctagccggca agcagagcgg
      181 ggcgccttta ggcgggcgag acggcagaca agaacctggc catggaggcg cgacagactt
      241 attgcgctct tccaggcagc cggcctcact ccacctgcag ccgaaaccag acaaatagca
      301 ttggctctgc ggcgggaagc agtgtcccgg cctttggctc cagatgccct ctgggccgcc
      361 ctcttgcatt tggctcacca tagggggttt aggagtaata ggattgacaa gcgcgagcgg
      421 gcggccgcaa aggcactcgc caaggcaaag cccgctaaag cgacagcaaa agccacagcc
      481 ccggccaagg aagctgacga tgaggccggc ttctgggaag gagcagaggc cgcacttagg
      541 cagagaatgg ctgcaagtgg tgcaccgact gtgggggccc tgctcgctga cgacctggac
      601 agagggcaac ccgtgcgcat gcgctacaat cagtcagata gagatggcgt ggtggctccg
      661 acacgcgctc tgatcgcaga ggaacttgcg gagattgtgg ccagacagtc ctcagcctac
      721 ccgggcctgg attggccagc ggtgactcgc ctggtacttg accagcgccc cctgcggagc
      781 aagggagctg gaccctgcgc ttttctgcca ggagaggacc gggccctgcg ggccctcccc
      841 acagtgcagg attttataat aagacagacg cttgcgaacc tgcgcttgcc aagtacgtcc
      901 gccgacgaac cccgcccttt gaccgacgag gagcacgcta aggccctcgc actgttgagc
      961 actgcccggt tcgtggagtg gcctgccctg agacgcgccc tgggcctgaa aagaggagtg
     1021 aaatttacgg cagaaaccga acggaatgga gcaaagcagg cagcgcgcgg cactgcaggg
     1081 aatctcacag aagccatcct ggcacccctg atccccggtt ggagcgggtg ggacctcgac
     1141 cggaaagaca gggttttcag tgacctctgg gccgcacgac aggaccggtc cgcccttctt
     1201 gctctgattg gagatccccg ggggcccact cgcgtgaccg aagatgagac tgccgaggcc
     1261 gtggccgacg ccatccagat agtgttgcct actgggcgag cctcactcag cgctaaggcg
     1321 gcgcgcgcca tcgcgcaggc gatggcccca gggataggtt atgacgaagc ggtcactctt
     1381 gcgctcggcc tccaccattc tcacaggccc cggcaggagc gattggcccg gctgccgtac
     1441 tacgcagcgg cgctgcccga tgtcggtctg gacggtgacc ccgtgggacc accacccgcc
     1501 gaagatgacg gtgcagcagc ggaagcatat tacgggagga taggaaatat atctgttcac
     1561 atcgcgctga acgaaacacg gaagatcgtt aatgcacttc tgcatagaca tggacctatc
     1621 ctccgcctgg tgatggttga aaccaccaga gagctgaagg cgggggccga tgagcgaaag
     1681 cggatgatag cagagcaggc cgaaagggag cgggagaacg ctgagatcga cgtggaactg
     1741 cggaagtccg accgatggat ggctaatgcc cgcgaaagac gacagagagt gcggctggcg
     1801 cgccgccaaa acaacttgtg cccctacaca tctacaccca tcgggcacgc agatttgctg
     1861 ggcgacgctt acgatatcga ccatgtcatt cctctggctc gaggaggccg cgacagtctc
     1921 gataacatgg ttctctgcca atccgatgcc aacaaaacaa aaggagacaa aaccccctgg
     1981 gaagcatttc acgacaaacc tggctggatt gcgcagaggg acgacttttt ggcccgcctg
     2041 gacccccaaa ctgctaaggc cctggcatgg cgatttgcgg atgacgctgg cgagcgcgtg
     2101 gctaggaagt cagcggagga cgaagaccag gggttcctgc ctcggcaact gactgacacc
     2161 ggatacatcg cgagagtcgc cttgcgctat ctctcactgg ttacgaatga accaaacgca
     2221 gtggttgcaa ctaacggccg gctgactgga ttgcttcgcc tggcttggga tatcacgcca
     2281 gggcccgcac ctagagatct gctgcccact cctcgcgatg cactgcgcga cgacactgcg
     2341 gcaagacggt tcctcgacgg cctgaccccg ccaccccttg ccaaagccgt cgagggagcc
     2401 gtgcaggccc gcctggcagc tctggggcga tcacgggtgg cagacgctgg gctggccgac
     2461 gctctgggcc tgacgctggc ttcactcggc ggtggcggga agaatcgcgc agaccaccgc
     2521 caccacttta tcgatgccgc catgatcgcg gtcacaacaa gaggacttat caaccagatt
     2581 aatcaggctt caggtgcagg tcgcattctg gatcttcgga aatggccccg gacaaacttt
     2641 gaacctccct acccaacgtt tagagcagaa gttatgaagc agtgggacca tattcaccct
     2701 agcattcgcc ctgcacaccg cgatggcgga agtctgcatg cagcaactgt ctttggagtg
     2761 cggaaccggc ccgacgcaag ggtgcttgtg cagcgaaaac ccgttgaaaa gttgttcttg
     2821 gatgccaatg ccaaacctct ccccgccgac aaaatcgcag aaattatcga cggcttcgcg
     2881 tctccccgaa tggccaaaag gtttaaggct ctgctggctc ggtaccaggc cgcccacccc
     2941 gaggtcccac ccgctctggc ggctctcgcc gtcgcgagag atcccgcttt tggtcccagg
     3001 ggaatgactg ccaacacggt gatcgcaggc cgaagcgacg gcgatgggga agacgctgga
     3061 ctgatcactc cgttcagagc taaccctaaa gccgcggtgc ggacaatggg gaatgctgtg
     3121 tacgaggtgt gggagatcca ggtgaaaggt cggcctagat ggacgcatag agtcttgact
     3181 aggttcgaca gaacccaacc tgccccccct cctcctcctg agaacgcccg cctcgtcatg
     3241 cgactgagac ggggagatct ggtatactgg cctctggagt ctggagatcg gctgttcctc
     3301 gtaaagaaaa tggcggtgga cgggaggctt gctctttggc ccgcccgctt ggctacagga
     3361 aaggcaacag ccctgtacgc acaactgtca tgccccaata tcaacctcaa cggggatcag
     3421 gggtactgcg tccagtccgc cgaggggatt cggaaggaga aaattcggac cactagctgt
     3481 accgccctgg gtcgactgcg actttcaaag aaggctacg
//
