LOCUS       sequence-195285-        3363 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q03LF7
FEATURES             Location/Qualifiers
     source          1..3363
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtcagatc ttgtcttggg cctggatatc ggcataggat ctgtcggcgt tggcatactc
       61 aacaaggtca ctggagagat tatccataaa aactctagga tctttcctgc cgctcaggcc
      121 gagaacaact tggtgcggag gactaaccgg caaggcagac ggcttgcccg gcgaaaaaag
      181 caccgcaggg tgagactgaa tcgccttttc gaagagagtg ggcttataac cgacttcact
      241 aaaattagca ttaaccttaa cccgtatcag ctcagagtta aaggactgac agacgagctc
      301 tcaaacgagg agctttttat tgccctgaaa aacatggtga aacacagggg catttcctac
      361 ttggatgacg ccagtgacga cgggaactcc agcgtgggcg actatgctca gatcgtgaaa
      421 gagaatagta agcaactcga aactaagaca ccaggccaaa tccagctgga acgctatcag
      481 acctatggac agctgcgcgg agacttcaca gtggagaaag acggcaagaa gcatagactc
      541 attaatgtgt ttccgacgtc agcataccgg tccgaagccc tgcgaatatt gcagacacaa
      601 caggagttca atcctcagat caccgacgag tttatcaatc ggtaccttga gattttgacc
      661 gggaagcgca aatactatca tgggccaggc aacgaaaagt cacgaaccga ctatggccga
      721 taccgaacca gtggagaaac tctcgacaac atcttcggaa tcctgatagg caagtgtacc
      781 ttctacccag acgagttccg ggctgccaag gcaagttaca ccgcccagga gttcaatctg
      841 ctgaacgacc tgaataacct cactgttccc acagaaacca agaagttgtc taaagaacag
      901 aaaaaccaga tcatcaatta cgttaaaaac gaaaaggcca tgggccccgc taaactgttt
      961 aagtacatag cgaaattgct gtcctgtgat gtagctgaca tcaaaggcta tcgcattgat
     1021 aagtcaggca aagccgaaat ccataccttc gaggcctaca gaaagatgaa gaccctggag
     1081 acacttgaca tcgaacaaat ggacagagag acactggata aacttgccta tgtcctgaca
     1141 ctcaatactg agagagaggg aatccaagaa gccctggaac acgagtttgc agatggatct
     1201 ttctcacaga agcaggtgga tgagttggtg cagtttcgca aggcgaattc aagcattttt
     1261 gggaagggct ggcataactt ctcagtcaaa ctgatgatgg agctcatccc agagttgtac
     1321 gagacttcag aggagcaaat gaccatattg acccggctgg gaaaacagaa aactacttct
     1381 tccagcaata agacaaaata tatcgatgag aaactcctga cagaagagat ctacaatcct
     1441 gtggtggcta aatccgtgcg ccaggccatc aagatcgtca atgcagccat aaaggagtac
     1501 ggcgacttcg ataatattgt gatagaaatg gctcgcgaaa cgaacgagga tgacgaaaaa
     1561 aaagctattc agaagataca gaaggctaat aaggacgaaa aagacgcggc gatgctgaag
     1621 gcggccaacc agtataacgg aaaggccgag ctgccgcact ccgtcttcca cggtcacaaa
     1681 cagcttgcca caaaaatcag gctgtggcat cagcaaggcg aacgctgcct ctacacaggc
     1741 aaaactatca gtatacacga tctgatcaat aactccaatc agtttgaggt cgaccacatc
     1801 ctgcccctct ccatcacgtt tgacgactcc cttgccaaca aagttcttgt gtacgccacc
     1861 gctaaccaag agaaagggca gcgcacccct taccaggccc tggactccat ggatgatgcc
     1921 tggtcattcc gggaactgaa agcctttgtg cgggagtcaa agacactctc taacaagaag
     1981 aaggaatacc tgttgactga ggaagacatt agtaaattcg atgtccggaa gaagttcata
     2041 gaacgaaatc tggtggatac tcgctatgct tcacgcgtcg ttctgaacgc cctgcaggag
     2101 cacttccgag ctcataagat tgacaccaag gtcagcgtgg tacgagggca gttcacatct
     2161 caactgagaa gacactgggg tatcgagaag acgcgagata cctatcacca ccacgcggtg
     2221 gacgccctta taattgcggc ctcttcccag ctcaatttgt ggaagaagca aaaaaatacc
     2281 ctcgtcagct acagtgaaga tcaactgctg gacatcgaga ctggggaact catttccgac
     2341 gacgaatata aagagagtgt cttcaaagcc ccataccagc atttcgtgga tacactgaag
     2401 tccaaggagt tcgaggactc catcctgttc agttaccagg tggactccaa gttcaatcgg
     2461 aaaatctcag atgcaaccat ttatgccaca aggcaggcaa aggtcgggaa ggataaagcc
     2521 gacgagacat atgtgctcgg gaagattaaa gacatttaca cccaagacgg gtacgacgca
     2581 ttcatgaaaa tatacaaaaa ggataagtca aaatttctga tgtatcggca tgatccacaa
     2641 acgttcgaaa aggttattga acctatcctc gaaaattatc ctaataagca gatcaatgag
     2701 aaggggaagg aagtgccttg taaccctttt ttgaaataca aagaagagca cggctacatc
     2761 cgcaagtact ctaaaaaagg taacggccct gagataaaat cactgaagta ttacgactct
     2821 aaactgggga accatattga catcacgcct aaagattcca ataataaagt tgtgctccag
     2881 tctgtgtccc cgtggcgggc cgacgtctac ttcaacaaga ccactggaaa atatgagatc
     2941 ctggggctta aatacgcaga tttgcagttc gaaaaaggaa caggaaccta taagatatct
     3001 caggaaaaat acaatgacat taaaaagaag gagggggtcg actcagactc agaatttaaa
     3061 tttacccttt acaaaaacga tttgttgctc gttaaagata cagaaaccaa ggagcagcag
     3121 ctgttccggt ttctgtccag aacaatgcca aaacagaaac actacgtcga gttgaaacct
     3181 tacgacaaac agaaattcga ggggggggag gccctcatca aagtcctcgg aaacgtggcc
     3241 aactccggcc agtgtaaaaa aggactgggg aagagtaata tcagcatata taaggtcagg
     3301 actgatgtgc tcggcaatca gcatattatc aagaacgagg gggataaacc aaaactcgac
     3361 ttt
//
