LOCUS       sequence-195287-        3912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q4A5I2
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctgaaca acgtgcaaaa cctgtggaag tatcttctct tgcttatctt tgacaagaaa
       61 attatttttc tgttcaagat caaggtaatc ttgattcgca ggtacatgga gaacaacaat
      121 aaagaaaaaa ttgtgatagg gtttgatctg ggtgtggcct cagttggttg gagtatcgtg
      181 aatgctgaaa caaaagaggt catcgatctg ggcgtgcgac tgttcagtga gcctgagaag
      241 gcagattaca ggcgggctaa gcgaaccaca cgcagactgc tgcgaaggaa aaagtttaaa
      301 agagagaaat ttcacaaact gatactgaaa aacgcagaga ttttcggact tcagagtagg
      361 aacgaaattc tgaacgtcta taaggatcag agctctaagt atcgcaacat ccttaaattg
      421 aaaatcaacg ccttgaaaga agagatcaaa cccagtgaac tggtttggat tctccgggac
      481 tatctgcaaa atcggggata cttctacaaa aatgaaaagc tgacagacga attcgtttcc
      541 aatagttttc ctagcaagaa gcttcacgag cactatgaaa aatacggatt ctttagaggg
      601 agcgtgaagt tggacaataa gcttgacaat aaaaaggaca aggccaagga gaaggacgag
      661 gaagaggagt ctgatgccaa aaaagagagt gaagagctga tcttcagtaa caaacagtgg
      721 attaacgaga tcgtcaaagt gttcgagaac cagtcttacc tcacagagtc cttcaaagaa
      781 gagtacttga aactgtttaa ttatgtgcga cccttcaata aaggccccgg gagcaagaat
      841 agccgcacag cgtacggagt ctttagcacc gatatcgacc ccgaaaccaa taagtttaaa
      901 gactacagca atatatggga taaaacaatc ggcaagtgca gtttgttcga agaggagatt
      961 cgagccccta agaacctccc gtccgccctg atctttaacc ttcagaacga gatttgtact
     1021 atcaaaaatg agttcactga attcaaaaac tggtggttga atgcagaaca gaaaagcgaa
     1081 atcctgaaat tcgtctttac cgagcttttc aactggaagg acaagaagta tagcgacaaa
     1141 aaatttaata agaatctgca agataaaatt aagaagtacc tcctcaactt cgctctggag
     1201 aactttaatc tgaatgagga gatacttaag aatcgcgatc tggagaacga taccgtgctg
     1261 ggcctgaaag gtgtaaagta ctacgagaag tcaaacgcca ccgccgatgc cgccctggag
     1321 ttttcctcct tgaaacctct ctacgtattt ataaagtttc tcaaggaaaa aaaactggac
     1381 ttgaactatc ttcttggcct cgaaaatacc gagattcttt actttctgga ctccatatac
     1441 ctggctatct cttactccag tgacctcaag gaacgcaatg agtggtttaa gaagctgttg
     1501 aaggaactct acccaaagat caaaaacaac aacctggaaa ttatcgagaa cgtggaggac
     1561 attttcgaaa tcacagacca ggagaagttt gaaagctttt ccaagactca cagtctgagc
     1621 cgagaggcat ttaatcacat cataccactc ctcctcagca acaatgaggg aaagaattac
     1681 gagtcactca agcattccaa cgaagagctg aagaagagaa ctgagaaagc agagttgaaa
     1741 gcccagcaga atcagaagta tctgaaagat aacttcctga aggaagcatt ggtgcccctg
     1801 tccgtcaaga cttctgtgct gcaggccatt aaaatattca accagattat caagaatttt
     1861 ggaaagaaat atgagatttc ccaagtggtg atcgagatgg ctcgggagct taccaagcct
     1921 aatctcgaaa agctcctcaa caacgccacg aattccaaca ttaagattct gaaggagaag
     1981 ctggaccaga cagagaagtt tgacgacttt accaagaaga agttcataga caaaatcgag
     2041 aactcagtcg ttttccggaa taaactgttt ttgtggttcg agcaggatcg caaagacccc
     2101 tacacacagc ttgacatcaa aatcaatgag attgaagatg aaaccgagat agatcatgtg
     2161 atcccctact caaaatccgc cgacgattcc tggttcaaca aactgctggt gaaaaaaagt
     2221 actaatcagc ttaaaaaaaa caaaacagtg tgggaatact atcagaatga gtccgaccca
     2281 gaggcgaaat ggaataagtt tgttgcatgg gctaaacgaa tttacctggt gcagaaatct
     2341 gataaggagt ccaaggacaa ttccgaaaag aatagcatat ttaagaataa gaaaccaaac
     2401 ctgaagttta agaatatcac caaaaaactt ttcgatccat acaaagactt gggcttcctg
     2461 gccagaaacc tgaatgatac acgctacgcc actaaggttt tcagagatca actgaacaac
     2521 tactctaagc atcattctaa ggatgatgag aataagctgt ttaaagtggt atgtatgaat
     2581 ggtagtatta cctcctttct gcgcaaatcc atgtggagga aaaacgaaga acaggtgtac
     2641 aggttcaact tttggaagaa agaccgggac caatttttcc accatgccgt agacgcctct
     2701 attattgcca tcttctcact gctgaccaag accctttaca ataagttgcg cgtgtacgag
     2761 agctatgatg ttcagaggcg ggaagacggg gtttatttga taaacaagga aactggggag
     2821 gttaagaagg ccgacaaaga ctactggaag gaccagcaca actttttgaa aatcagggaa
     2881 aatgccattg aaatcaagaa cgtgctgaac aatgtggatt ttcaaaatca agtgagatac
     2941 agtcggaagg cgaacaccaa gttgaacaca cagctcttta acgagacgct gtatggcgta
     3001 aaagagttcg agaacaattt ctacaagctg gaaaaagtga acttgttctc aagaaaggat
     3061 ctgaggaaat ttatactgga ggacctcaac gaagagagtg aaaaaaataa gaaaaacgaa
     3121 aatggttcca gaaaacggat cctgactgag aagtatatcg ttgacgaaat cctgcagata
     3181 ctggaaaatg aagaatttaa agactctaaa tctgacataa atgccctcaa taaatacatg
     3241 gacagccttc ccagcaagtt ttccgagttt ttttcacagg acttcattaa taaatgcaaa
     3301 aaggagaatt ccctgattct gacgttcgac gctataaaac ataacgaccc caagaaagtg
     3361 atcaaaatca agaatcttaa gttcttccgc gaggatgcca cccttaagaa taaacaagct
     3421 gttcacaagg actcaaagaa tcagatcaag tccttctacg agtcctacaa gtgcgtgggg
     3481 ttcatttggc tgaagaataa aaacgatctg gaggagagca tattcgtccc aattaactca
     3541 cgagtgatcc actttggtga caaggacaag gacatttttg actttgactc ttataacaag
     3601 gaaaagttgc tcaacgaaat caacctcaag cggcccgaaa ataagaagtt taactcaatt
     3661 aacgaaattg agttcgttaa atttgttaaa ccgggtgcac ttcttcttaa ctttgagaac
     3721 cagcaaattt actatatcag caccttggaa agcagctccc tccgcgcgaa aatcaagctg
     3781 ctcaacaaga tggataaagg gaaggctgtg agtatgaaaa aaattactaa ccccgatgag
     3841 tataaaataa ttgaacacgt gaacccactg ggaattaatc tgaattggac aaagaaattg
     3901 gagaacaaca at
//
