LOCUS       sequence-195289-        4308 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q5L8F8
FEATURES             Location/Qualifiers
     source          1..4308
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaaacgaa ttctgggcct ggatttggga acaaactcta ttggctgggc gctggtcaat
       61 gaggccgaga acaaagacga gcggagtagt attgtgaaat tgggcgtgag agttaaccca
      121 ttgactgtcg atgaactgac gaattttgag aaaggcaaaa gtatcacgac caatgctgat
      181 cgcacgctta agcggggaat gcggaggaac ctccagcgat acaaactgag gagagagaca
      241 ttgacagagg ttttgaaaga gcacaaactg atcactgagg atacaatcct gagcgagaac
      301 ggcaacagaa caacttttga aacctaccgc ctgcgggcaa aggccgtaac cgaggagatc
      361 tctctcgagg agtttgccag agtcttgctt atgataaaca agaagcgcgg atataagtct
      421 tcccggaagg cgaagggcgt agaggaggga actcttatcg acgggatgga catcgcccgg
      481 gagctgtaca acaataatct gactccgggg gaactctgtc tgcagttgct ggacgcaggc
      541 aagaagtttc ttccagattt ttatcggtcc gatttgcaaa acgagctgga tcggatctgg
      601 gaaaaacaga aggagtacta tccagagatt ctgacagatg ttctgaaaga ggagttgaga
      661 ggcaaaaagc gcgatgccgt atgggccatt tgcgccaagt acttcgtttg gaaggaaaat
      721 tacactgagt ggaataagga gaagggaaaa acggagcagc aagaacggga acacaagctg
      781 gagggcatct actccaagag gaaacgggat gaagcgaaga gagagaactt gcagtggcgg
      841 gtcaatggac ttaaggaaaa gctgagtctg gagcagctcg tgatcgtctt ccaggaaatg
      901 aatacacaga ttaataacag ctcaggctac ctgggcgcta tcagtgacag atcaaaagag
      961 ttgtacttca ataagcagac tgtcgggcag tatcagatgg agatgttgga taagaatccc
     1021 aacgcaagtc ttaggaacat ggtgttctac aggcaagact acctggacga atttaacatg
     1081 ttgtgggaaa agcaagcggt ctaccacaag gagctgaccg aggagctgaa gaaagagatc
     1141 agagatatta tcatcttcta ccagcgcagg cttaagtccc agaaaggcct gatagggttc
     1201 tgcgagtttg agagccggca aatcgaggtg gacatcgatg gcaagaagaa aatcaaaacc
     1261 gtggggaaca gagtgatatc cagaagctcc cctctgttcc aggaatttaa aatctggcag
     1321 atacttaaca atatagaagt gaccgttgta gggaagaaac ggaagagacg aaagctgaaa
     1381 gaaaactact ctgccctctt cgaagagctt aacgacgccg aacagctgga gctcaacggc
     1441 agtagacggc tgtgccaaga ggaaaaagag ctgctggcac aggagctgtt cattcgggac
     1501 aagatgacga agagtgaagt gttgaagctg ctgttcgaca atccacaaga attggacctt
     1561 aacttcaaaa ctatcgatgg aaataagact ggctacgctt tgttccaagc ctattctaag
     1621 atgattgaga tgagcggaca tgaacccgtg gactttaaga agccagttga gaaggtggtg
     1681 gagtacatta aggccgtctt tgatctgttg aattggaata ccgacatcct gggctttaat
     1741 tcaaacgagg aactcgacaa ccagccctat tataagctct ggcatttgct ttatagcttt
     1801 gagggagata acacaccaac aggaaacgga agactgatcc agaaaatgac tgagctttac
     1861 gggtttgaaa aggaatacgc aactatcctg gcgaacgtgt ctttccagga tgattacggg
     1921 tccctctctg ctaaagctat ccacaaaatt ctgcctcatc ttaaagaagg aaatagatac
     1981 gatgtcgcct gcgtgtatgc agggtacaga cactctgaat ctagcctgac ccgcgaagaa
     2041 atcgctaata aggttcttaa agaccggctg atgctgcttc ctaaaaactc tcttcataac
     2101 ccagtagtcg aaaagattct taaccagatg gtgaatgtta ttaatgttat catcgacatc
     2161 tacggaaaac ccgacgagat cagggtagag ctggctcgcg agctgaagaa gaatgctaaa
     2221 gagcgcgagg aactgacaaa gagcatcgcc caaaccacta aagcacacga ggagtataag
     2281 accctcttgc agactgagtt tgggctcaca aacgtgtcta gaacggacat tcttagatac
     2341 aaactgtaca aggagcttga atcatgcggt tataaaaccc tctatagcaa tacctacatt
     2401 tcacgcgaga agctgttttc caaggagttc gatattgaac acattatacc acaggccaga
     2461 ctgtttgacg actctttctc caataagaca ctggaagcta ggagcgtgaa tatcgagaaa
     2521 ggtaataaaa cagcttacga cttcgtcaag gagaagtttg gggaatctgg cgccgacaac
     2581 tccctcgaac actatttgaa caatattgag gacctgttca aaagtgggaa aatctcaaag
     2641 acaaagtaca ataagctcaa aatggctgag caggatattc ccgatgggtt cattgagaga
     2701 gatcttagaa acacacaata tatcgccaaa aaagccctga gtatgctgaa cgagatcagc
     2761 cacagagtgg tcgccacctc tggatcagtt accgataagt tgcgcgaaga ctggcagctg
     2821 atcgatgtca tgaaagagct taactgggaa aagtataagg ccctgggact ggtagagtac
     2881 ttcgaggacc gcgacggaag gcagattgga agaataaaag actggaccaa gcggaatgac
     2941 cataggcacc acgctatgga tgcccttaca gtcgcattca caaaagacgt gttcatccaa
     3001 tatttcaaca acaaaaacgc cagtctggac ccaaacgcta atgagcacgc tattaagaac
     3061 aagtatttcc agaacggccg agccattgcc ccaatgcccc tgcgggagtt tcgggccgaa
     3121 gctaagaaac acctcgagaa caccctgatc agtatcaaag caaaaaacaa ggtgataacc
     3181 ggcaacatta acaagacccg caaaaaaggc ggggttaata aaaacatgca gcaaaccccc
     3241 cggggccaat tgcacctcga aacaatctac gggtctggca agcagtacct caccaaggaa
     3301 gagaaggtta atgcgtcatt cgacatgagg aaaataggaa ctgtgtcaaa atccgcttat
     3361 agggacgcac tgttgaaacg gctgtacgag aacgataatg accctaagaa ggcctttgca
     3421 ggcaaaaatt ctctcgataa gcagccaatc tggctggaca aagagcaaat gcggaaagtc
     3481 cctgaaaagg tgaagattgt gactttggaa gctatctaca ccattaggaa agaaattagt
     3541 cctgacctca aggtagataa ggtaatcgac gtgggtgtcc ggaagatcct gattgacagg
     3601 cttaacgagt acgggaacga cgctaagaag gccttctcca atttggataa gaacccgatc
     3661 tggcttaata aggagaaggg gattagcata aagcgggtga caattagcgg gatttccaac
     3721 gctcagtccc tgcacgtcaa gaaggataag gacggcaaac cgatcttgga tgagaatgga
     3781 cggaatatac ctgtcgactt cgttaacacc ggcaacaacc accatgtcgc tgtctactac
     3841 cgccccgtta tcgacaaacg gggccagctt gtcgtggacg aggcgggcaa tccgaaatat
     3901 gagctggaag aggttgtggt ttccttcttt gaagccgtga cccgagccaa tctgggactc
     3961 ccgattatcg acaaggacta caagaccact gaaggctggc aatttttgtt cagcatgaag
     4021 cagaacgaat acttcgtctt cccaaacgag aagaccggct tcaacccaaa ggaaatcgac
     4081 ctcctcgatg tcgaaaacta tggcttgatc tcccctaacc tgttcagagt tcaaaagttt
     4141 tcactgaaga attatgtatt ccggcaccat ctggagacaa cgattaagga cactagcagc
     4201 atactgcgag ggatcacctg gattgacttt aggtccagta aaggtttgga cactatcgtc
     4261 aaggtgcgcg tgaaccatat cggacagata gtgtccgtgg gagagtac
//
