LOCUS       sequence-195291-        4116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q5X8T6
FEATURES             Location/Qualifiers
     source          1..4116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggagtcta gtcaaattct ttctccgatt ggaattgatc tggggggtaa attcacaggc
       61 gtctgtctga gccacctcga ggcgttcgct gagctgccca atcacgcaaa tacgaaatat
      121 tccgtgattc tgattgacca taataacttc caactgtctc aggcacagcg ccgcgcaacg
      181 agacataggg taagaaataa aaaaaggaat caatttgtaa agcgagttgc actgcagctg
      241 ttccaacaca ttctttccag agatctgaat gcaaaggagg aaaccgccct gtgccactat
      301 ttgaacaacc gagggtatac ttacgtggac accgacctgg acgaatacat caaggatgaa
      361 actacaatta accttctcaa agagctcctg ccttctgagt ccgaacacaa ttttattgac
      421 tggttcctgc agaagatgca gagttccgag ttcaggaaga tcctggtgag taaagtcgag
      481 gagaagaagg atgataaaga gctgaaaaat gctgttaaaa acattaaaaa ttttatcacc
      541 ggatttgaaa agaattctgt cgaggggcac cggcatagga aagtttactt tgaaaacatt
      601 aaatccgata tcaccaagga taaccagctg gattccatta aaaagaaaat acctagtgtc
      661 tgcctgtcca atcttctcgg ccacctctct aatctgcagt ggaagaatct gcacaggtac
      721 ctggctaaaa accccaaaca gttcgacgag cagaccttcg gtaacgaatt tttgcgcatg
      781 ttgaagaact tcagacattt gaaggggtcc caggaatcac tcgccgtgag aaatctgatc
      841 cagcagctgg agcagtcaca ggattacata tcaattcttg agaagacccc cccagaaatc
      901 acaatccccc cttatgaagc ccgcacaaat acgggaatgg agaaggatca gagtctgttg
      961 ttgaatcctg aaaagctgaa caacctttac cccaactggc gcaacctgat ccctgggatt
     1021 atcgatgcac acccatttct ggaaaaagac ttggaacaca cgaagctccg agatcgcaag
     1081 cgaatcatca gtcccagcaa acaggacgaa aagcgggaca gctacattct gcagaggtac
     1141 ctcgacctga ataaaaagat tgataagttc aaaataaaga agcagttgag ctttctgggg
     1201 cagggcaaac agcttcccgc caatctcatc gaaacgcaga aggagatgga aacccatttt
     1261 aacagctcac tggtgagcgt gttgatacag attgcatcag cttataataa agaaagggag
     1321 gacgcagccc aggggatatg gtttgataac gccttctccc tctgtgaact gtccaacatt
     1381 aatcctcccc ggaagcagaa gattctgccc ctgctggtgg gagccatact gagtgaagac
     1441 tttatcaata acaaagacaa gtgggctaaa ttcaaaatct tctggaacac tcataaaatc
     1501 ggtcgcacct cccttaagag caagtgcaaa gaaatcgagg aagctcggaa gaacagtggt
     1561 aatgcgttta agatcgacta tgaggaggcc ttgaaccacc ctgaacactc aaacaataaa
     1621 gcccttatca agattatcca gactatccca gacataatcc aggctatcca atcccacctg
     1681 gggcacaatg attctcaagc actgatttac cataatccct tttcactgag tcaactgtat
     1741 actatccttg agactaagcg agatgggttc cacaagaact gcgtggccgt gacgtgtgag
     1801 aattactggc ggagtcagaa gaccgaaatc gacccggaaa tctcttacgc ctcaaggctt
     1861 ccagctgata gcgtgcgccc tttcgacggc gttctggctc ggatgatgca gcggcttgcc
     1921 tacgagatag cgatggccaa atgggaacaa atcaaacaca ttccagacaa ttcaagtctg
     1981 cttatcccca tctatctgga acagaaccgg ttcgagtttg aggagtcgtt taagaaaatc
     2041 aagggctctt ctagcgacaa aactctggaa caggctatcg agaagcagaa cattcagtgg
     2101 gaggagaaat tccagagaat cattaatgcc agtatgaata tctgccccta taagggggca
     2161 agcatcggcg ggcaggggga aattgatcat atatatccta gaagcttgag taagaaacac
     2221 tttggagtga tctttaacag cgaggttaac ttgatctatt gtagtagtca ggggaataga
     2281 gagaagaaag aggagcatta cctgctcgaa catcttagcc ctctgtacct caagcaccag
     2341 ttcggaaccg ataacgtctc tgatattaaa aactttatct cccagaatgt tgcaaatatc
     2401 aagaagtaca tctccttcca ccttctgacc cctgagcagc agaaggcagc cagacatgcc
     2461 ctgttcctgg attatgacga tgaggccttc aagaccatta cgaaattctt gatgagtcaa
     2521 cagaaagctc gagtgaatgg tactcagaag tttctgggga agcaaatcat ggaattcttg
     2581 tccaccctgg cggattccaa gcagctgcag ctggagttca gtataaaaca gatcacagct
     2641 gaggaggttc acgaccatag ggagctgctg tccaagcagg aacccaaact cgtgaagtca
     2701 cggcagcaga gcttccctag ccacgccatt gatgccacgt tgacaatgag catcggactc
     2761 aaggagttcc ctcaattttc acaagaactc gataatagct ggttcattaa ccacctgatg
     2821 cctgacgagg tccacctgaa cccagtgcgc tccaaggaaa aatacaataa acctaatatc
     2881 agtagcaccc cactcttcaa ggacagtctc tacgctgagc gcttcattcc cgtgtgggtc
     2941 aaaggggaaa cctttgctat cggattcagc gaaaaggatc tgtttgaaat caagccctcc
     3001 aacaaggaga agctgttcac gctgctgaag acatacagca caaagaatcc tggggagtca
     3061 cttcaggagc tgcaggcaaa gtccaaagcg aaatggctgt atttccccat aaataaaacc
     3121 ctggccctgg agtttctgca tcattatttt cacaaggaga tcgtgacccc tgatgacacc
     3181 acggtgtgtc actttatcaa ctctctgcgg tattacacca agaaggagag tattacggtg
     3241 aagatattga aagagcccat gcccgtgctg tccgttaagt tcgagtcctc aaagaagaat
     3301 gtgctcgggt cattcaagca tacaatcgcc ctgcccgcga caaaggactg ggagcgattg
     3361 tttaatcacc ccaacttttt ggccctcaag gcaaaccccg cccctaatcc caaagaattt
     3421 aacgaattca ttcggaagta tttcctttca gacaataatc caaacagcga tattccaaat
     3481 aatgggcaca acatcaaacc ccagaaacac aaagcagtta gaaaggtgtt ttccttgccc
     3541 gtcatacccg gcaacgctgg taccatgatg aggattcgcc ggaaggataa caagggccaa
     3601 cctctttacc agctccagac aatcgacgac acgccaagca tgggaatcca gatcaacgaa
     3661 gatcggctgg tgaaacagga agtgcttatg gacgcatata agacgcggaa tctctctacc
     3721 attgacggca taaacaacag cgagggacaa gcttacgcaa cctttgataa ctggctgact
     3781 ttgcccgtgt ccacttttaa gcctgagata atcaagctgg aaatgaaacc acactccaag
     3841 acccgcagat acatccggat tactcagtca ctggccgatt tcatcaaaac aattgacgaa
     3901 gcgctgatga tcaagccctc cgacagtatt gatgatcctc tcaacatgcc taacgaaata
     3961 gtttgcaaga acaagctgtt tggtaacgaa ctgaagccac gcgacgggaa gatgaagatt
     4021 gtgtccacag gcaaaatagt gacttacgag tttgaatcag acagcacacc tcagtggatt
     4081 cagacgttgt acgtgacgca gctgaagaaa cagcca
//
