LOCUS       sequence-195293-        3708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q6KIQ7
FEATURES             Location/Qualifiers
     source          1..3708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtatttct acaagaataa agagaacaag ctgaacaaaa aggtggtgtt gggactcgac
       61 ctggggattg cctcagtggg gtggtgtctg actgacatct ctcaaaagga ggataacaag
      121 tttcctatca tcctccatgg agtccgcctg ttcgagacgg tggacgactc cgacgataag
      181 ctgctgaatg aaacacgaag gaagaagaga ggacagagaa gacgaaatcg gcggcttttc
      241 acccgcaagc gggattttat caagtacctg atcgacaata atattattga acttgagttc
      301 gacaagaatc cgaagattct ggtgcgcaat ttcatcgaaa agtatatcaa tccctttagt
      361 aagaatctcg aactcaaata taagagcgtt acaaatctcc ccatcgggtt ccataacctg
      421 cgcaaggccg caatcaatga gaaatacaag cttgacaaga gtgagctgat cgtgttgttg
      481 tatttttacc tgtcccttcg cggtgccttc ttcgataatc ccgaagacac gaagtctaaa
      541 gaaatgaaca agaatgagat cgaaattttt gataagaatg agtccattaa gaacgcggaa
      601 ttcccgatcg ataagataat cgaattttac aagatcagtg gaaagataag gtccacgata
      661 aaccttaaat tcggccacca ggactacctg aaagaaataa aacaagtgtt tgaaaagcag
      721 aacatcgatt ttatgaatta cgagaaattt gcaatggagg aaaaaagctt cttttcccgg
      781 ataaggaatt acagcgaagg acccggaaat gagaagtctt tctccaagta cggattgtat
      841 gcaaatgaga acggtaaccc cgagttgatc ataaacgaaa agggacagaa gatttataca
      901 aaaattttta aaacactgtg ggaaagtaaa atcggcaagt gtagctacga taaaaagctt
      961 taccgggctc ccaagaactc cttctcagca aaagtattcg atatcactaa caaactgact
     1021 gactggaaac acaaaaatga atatattagt gagaggttga aaagaaagat tttgcttagc
     1081 agattcctga acaaggatag caaatccgct gtggagaaaa tacttaagga ggagaacatt
     1141 aagttcgaga acctttctga aatcgcatat aataaggatg ataataagat caatcttccc
     1201 atcattaatg cataccacag cctgacaact atctttaaaa agcacttgat caactttgag
     1261 aattacttga tttctaacga gaatgatctg tctaagctga tgagctttta taagcaacaa
     1321 agcgaaaaac tgttcgtgcc taacgagaaa gggtcttacg agatcaatca aaataacaac
     1381 gtactgcaca tcttcgatgc catttccaat atccttaaca agttcagtac catccaggat
     1441 cgaattcgga tcctcgaagg gtactttgaa ttcagtaatc ttaagaagga cgtgaagagc
     1501 tctgaaatct attcagagat tgctaaactg cgagaattct caggaaccag ctccctgtct
     1561 tttggggctt actacaaatt tatccccaac ctcatctcag agggctccaa aaactacagc
     1621 accataagtt atgaggagaa agccctccag aatcagaaaa ataacttcag tcactctaac
     1681 ctgttcgaaa agacttgggt ggaggatctc attgcctccc ctaccgtcaa gcggtctttg
     1741 cgacagacta tgaacctgct gaaggaaatc tttaagtatt ccgaaaaaaa taacctcgag
     1801 atcgagaaga ttgtagtcga ggtaactaga agctctaaca acaagcacga aaggaaaaaa
     1861 attgaaggta taaacaagta tcgaaaagag aagtacgagg aactgaaaaa agtatatgat
     1921 ctccccaacg aaaacaccac tctgcttaag aagttgtggt tgttgcgcca gcagcagggc
     1981 tatgacgcat actcactccg gaagattgaa gccaatgatg tgattaataa gccttggaat
     2041 tacgatattg atcacattgt gcctaggtct atctccttcg acgattcatt tagcaatctg
     2101 gtaattgtga ataaactcga taatgcaaaa aaatcaaatg acctgtccgc caaacagttt
     2161 attgagaaaa tctatgggat cgagaagctg aaggaggcca aggaaaactg ggggaattgg
     2221 tacctgcgca atgctaatgg taaggctttc aacgataaag gtaagttcat caaactttat
     2281 accattgaca atctcgacga atttgataac agcgatttta ttaacagaaa ccttagtgac
     2341 accagctaca ttactaacgc tctcgtcaac catcttacct tttctaattc taagtataag
     2401 tattctgttg tctcagtgaa cggaaaacag acctccaatc ttcgcaatca gattgctttc
     2461 gtcgggatta agaacaacaa ggaaaccgaa cgcgagtgga agcgtccgga gggctttaag
     2521 tcaattaatt ctaacgattt cctgatacgc gaggaaggaa aaaacgatgt taaagatgat
     2581 gttctcatta aggataggtc ctttaacggg caccatgccg aggacgccta ctttatcaca
     2641 atcatctccc agtacttcag gtcctttaag cggatcgaga gacttaacgt caactacagg
     2701 aaagagacac gcgaactgga cgacctcgaa aagaataata tcaagtttaa agaaaaggcc
     2761 tccttcgata attttctgtt gattaatgcc ctggacgaac tgaatgaaaa actcaatcaa
     2821 atgcggttca gcaggatggt gatcacaaag aagaataccc agctgtttaa tgaaacactc
     2881 tatagtggga agtacgacaa gggcaagaac acaatcaaga aagtcgagaa gcttaacttg
     2941 ctggacaatc gcacagacaa aatcaagaaa atcgaagagt tctttgacga agataagctc
     3001 aaggaaaacg aattgactaa actccatata tttaaccacg acaagaatct gtacgaaacc
     3061 cttaaaatca tatggaacga ggtgaaaatc gaaatcaaga ataagaatct taacgagaaa
     3121 aattacttta agtacttcgt gaataagaag ctgcaggagg gcaagattag tttcaacgaa
     3181 tgggtgccga tcctggacaa cgacttcaaa attattcgaa aaattcggta tatcaagttc
     3241 tctagcgagg agaaagagac tgacgagatc attttttccc agtcaaactt ccttaaaatc
     3301 gatcaaaggc agaactttag cttccataat accttgtact gggtgcagat ttgggtctac
     3361 aaaaaccaga aagatcagta ttgctttatt tccattgacg ccaggaatag caagtttgag
     3421 aaagatgaaa tcaaaatcaa ctatgagaag ctcaagacac aaaaggagaa actgcagatc
     3481 ataaatgagg aaccgattct caagataaac aaaggtgatc tgtttgagaa cgaagaaaag
     3541 gaactgttct atatcgtggg cagagacgaa aaaccacaga agctcgaaat aaaatatatc
     3601 ctggggaaga aaatcaaaga ccaaaagcag attcaaaaac ctgtcaaaaa atactttccc
     3661 aattggaaaa aggttaatct tacatacatg ggggaaatct tcaagaaa
//
