LOCUS       sequence-195295-        4035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q8DTE3
FEATURES             Location/Qualifiers
     source          1..4035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaagaagc cttactccat cggcctggat ataggcacta actcagtggg gtgggccgtc
       61 gtcaccgacg actataaggt gcctgccaaa aagatgaagg ttctggggaa taccgacaag
      121 tctcatatag aaaaaaactt gctgggcgcc cttctgttcg attctggtaa taccgctgag
      181 gataggaggc tgaagagaac cgctcgcagg agatacactc ggaggagaaa ccggatcctt
      241 tatctgcagg aaatcttctc cgaggaaatg ggtaaagtag acgacagctt cttccacaga
      301 ctggaagatt cttttttggt gactgaggac aagaggggcg aacggcatcc tatttttggt
      361 aatctggaag aagaggtgaa gtatcatgaa aacttcccga ctatctacca tctgcgccag
      421 tacctggccg acaatcccga aaaagtagac cttcggctgg tgtacctggc tctggcccat
      481 attatcaagt ttcgcggtca ctttctcatc gaagggaaat ttgatacacg caataacgac
      541 gtccagcggc ttttccaaga gttcctggcc gtctacgaca acaccttcga gaacagctct
      601 ctgcaggagc agaatgttca ggtggaagaa atccttaccg ataagatctc taagtcagca
      661 aagaaagacc gggttctgaa actgttcccg aacgagaagt ccaacggtcg atttgcagaa
      721 tttctcaaac tgatcgtggg aaatcaagcc gactttaaga agcactttga actggaggaa
      781 aaagctccac tgcaattctc taaggatact tacgaagaag aacttgaagt gctgctcgcc
      841 cagatcggag acaattatgc cgagctcttc ctctcagcca aaaagctgta tgacagtatt
      901 ttgcttagcg ggatacttac cgtcaccgac gtcggcacta aagcacccct cagcgcctcc
      961 atgattcagc gatataacga acaccagatg gatttggcac aacttaagca gttcatccgg
     1021 cagaagctca gtgacaagta taatgaggtt ttctccgacg tcagtaaaga tgggtatgcc
     1081 ggctatattg atggcaagac aaatcaggaa gccttctaca agtacctgaa aggcttgttg
     1141 aacaaaattg agggcagcgg gtacttcttg gataaaatag aaagggagga ctttcttaga
     1201 aaacagcgca ccttcgataa tggctccata ccacatcaaa tccaccttca agaaatgagg
     1261 gccatcatca gacgacaggc agaattttac ccgtttctcg ctgacaatca ggacaggatt
     1321 gaaaagctgc ttacctttcg gattccctac tacgtggggc cacttgccag gggtaaatcc
     1381 gacttcgctt ggctcagccg gaagtcagcc gacaaaatca ccccttggaa cttcgatgag
     1441 atcgtggata aagagtcttc cgccgaggcg tttatcaatc ggatgacaaa ctacgacttg
     1501 tacctgccga atcagaaggt actgcccaag cactccctgc tgtacgaaaa gtttacagtg
     1561 tataacgaac tgaccaaggt taagtataaa acagaacaag gtaaaaccgc gtttttcgac
     1621 gccaatatga aacaggagat cttcgatgga gttttcaagg tctaccggaa agtaaccaaa
     1681 gataagctca tggatttcct ggagaaggag ttcgatgaat ttcgaatcgt tgaccttact
     1741 ggcctggaca aagaaaacaa ggtgttcaac gcttcttacg gaacctacca tgacctgtgc
     1801 aagatcctcg acaaggactt tctggacaac agtaagaatg agaagattct ggaagatatt
     1861 gtgctcaccc tgacactgtt cgaagaccgg gagatgattc gcaagcggct ggagaactac
     1921 agtgaccttc tgaccaagga gcaggtgaaa aaactcgaaa gaaggcacta tacaggctgg
     1981 ggtaggctct ccgctgaact tatccatggc atacggaata aagaatcacg caaaactatc
     2041 ctggattacc tgatagacga cggtaatagc aatcgaaact tcatgcagct catcaacgac
     2101 gacgcgctct ctttcaagga ggaaatcgcc aaagcccagg taataggaga aaccgacaat
     2161 ctcaatcagg tcgtatccga cattgccggg agccctgcta tcaaaaaagg aattctgcag
     2221 agcctgaaga tcgtcgacga actcgtcaag atcatgggcc accagcccga aaacatcgtt
     2281 gtagaaatgg cccgcgaaaa tcagttcacc aaccagggcc gccgaaatag tcaacagagg
     2341 ctgaaaggcc tgaccgatag tattaaggaa ttcggatcac agatcctgaa ggagcaccct
     2401 gtcgaaaata gccagctgca gaacgatagg ctgtttcttt attatctcca aaacggtagg
     2461 gacatgtaca cgggggaaga gttggacatc gactatctct cacaatatga catcgaccat
     2521 atcatcccgc aggcgttcat caaggataat agcatcgaca acagagtgtt gacctctagc
     2581 aaggagaatc ggggtaagtc cgacgacgtg ccgtctaagg atgttgtgag gaagatgaaa
     2641 tcctactgga gtaaattgct ttcagccaag cttatcaccc agaggaaatt cgacaacttg
     2701 accaaagccg aaaggggagg cctgaccgac gacgacaaag cagggttcat taaaagacag
     2761 cttgtggaga cgagacaaat caccaaacat gtagcgagaa tacttgacga acggttcaac
     2821 accgagacgg atgagaataa taaaaaaatt cgacaggtca agatagttac actcaagtca
     2881 aacctggttt ccaacttcag aaaagagttc gaactgtata aggtcaggga gatcaatgat
     2941 tatcaccatg ctcacgacgc ctatcttaat gcagtgatcg gcaaagcctt gctgggagtc
     3001 tatccccagc tcgagcccga gtttgtgtat ggcgattacc ctcacttcca tggccacaag
     3061 gagaacaagg ctacggctaa aaaatttttc tactccaata tcatgaactt cttcaaaaag
     3121 gacgatgtcc gcaccgacaa gaacggcgaa attatatgga aaaaagatga gcatattagt
     3181 aatatcaaaa aggtgctgag ctacccacaa gtaaatatcg tcaaaaaagt cgaggagcaa
     3241 acagggggtt tttctaaaga gagtattctg cctaaaggaa actctgataa gctcatccct
     3301 aggaagacaa aaaagttcta ttgggataca aagaagtacg ggggctttga ttcccctatt
     3361 gtggcttatt ccattctggt tattgccgat atcgagaagg gcaagtccaa gaagttgaag
     3421 acagtcaagg cactggttgg tgttacaata atggaaaaga tgacctttga gcgagatcca
     3481 gtggccttcc tggagcggaa gggctaccgc aacgtgcaag aggagaatat cattaagctg
     3541 cctaaataca gcctttttaa gttggaaaac gggcgaaaac gccttttggc ttcagcccgg
     3601 gaactgcaaa agggaaatga gattgtgctg cctaaccact tgggaactct gctgtaccac
     3661 gctaaaaata tacataaggt cgatgagcca aagcacctgg attatgtcga taagcacaaa
     3721 gacgagttca aggagttgct cgacgtcgtc tcaaatttct ccaaaaaata cacgttggct
     3781 gaggggaacc tggagaaaat caaggagctg tacgcgcaga acaacggcga ggacctgaaa
     3841 gaactggctt ctagcttcat aaatctgctg acatttactg ctattggagc accagcaacg
     3901 ttcaaattct ttgataaaaa tatcgaccga aagaggtaca cttccaccac cgaaattctg
     3961 aacgcaaccc tcatccacca gtcaatcact ggactgtatg aaacccgaat cgacctcaac
     4021 aagttgggcg gagac
//
