LOCUS       sequence-195297-        4185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q73QW6
FEATURES             Location/Qualifiers
     source          1..4185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaagaagg aaattaaaga ctacttcctc gggctggacg tgggtacagg ctctgtgggg
       61 tgggccgtca cagacaccga ctacaaactg ctcaaagcca atagaaagga tctgtgggga
      121 atgcggtgct tcgaaaccgc cgaaaccgct gaggtcagga ggctccaccg aggcgcccgc
      181 cgcagaatcg aacgccggaa gaaacgaatc aagctccttc aggagctttt ctcacaagag
      241 atcgctaaaa ctgacgaggg atttttccag cggatgaaag agtccccctt ctacgctgaa
      301 gataagacta tcctgcagga gaacaccctg tttaatgata aggattttgc cgataaaacc
      361 tatcataagg cttatcctac cattaatcac ctgattaagg cgtggatcga gaataaagtg
      421 aagcccgatc cgcgcctgct ctatctggca tgccacaata tcatcaaaaa gcgcgggcat
      481 tttctgtttg agggagactt tgatagtgag aatcagttcg acacctctat ccaagccctt
      541 tttgagtatc tgagagagga tatggaagtg gacattgacg cagatagcca gaaggtgaaa
      601 gagatcttga aggactcaag tcttaagaac tctgagaaac agtctagact gaacaagatc
      661 ctgggtctga agcccagcga caagcagaag aaagccatta ccaatcttat ctccgggaat
      721 aaaatcaatt ttgcagatct gtacgacaat cctgatctga aagatgccga aaaaaattcc
      781 atcagcttca gcaaggacga tttcgatgcg ctcagcgatg acctcgccag catcctcggt
      841 gattcatttg agttgttgct gaaggcaaag gccgtgtaca actgttctgt tctgtccaaa
      901 gtcatcgggg atgaacagta tctgagtttc gcgaaggtaa aaatatacga aaaacacaag
      961 accgacctga ccaagcttaa aaatgttatt aagaagcact tccctaaaga ctacaagaaa
     1021 gtgttcggat ataacaagaa tgagaagaat aacaataact actcaggata cgttggagta
     1081 tgtaaaacca aaagcaagaa actgataata aataatagtg tgaaccaaga ggacttttat
     1141 aagtttttga agaccattct ctcagcaaaa agcgagatca aagaggtcaa tgacattctg
     1201 accgaaatcg agacgggtac ctttctgccg aagcagatct caaaatccaa cgctgaaatc
     1261 ccatatcaac tgcggaaaat ggaactcgag aagatcctgt ccaatgctga gaaacatttc
     1321 agtttcttga aacagaagga cgagaagggg ttgtctcact cagaaaagat cattatgctg
     1381 ctcacgttta aaatccctta ttatatcgga cccattaacg ataaccataa aaaattcttt
     1441 ccggatcggt gttgggtcgt caaaaaggag aagtctccgt ctggaaaaac taccccctgg
     1501 aatttcttcg accacattga taaagagaag acagccgagg cctttataac tagtcgcacc
     1561 aatttttgta cctacttggt aggggagagt gtacttccaa aaagctccct gctgtactct
     1621 gaatacactg tactgaatga gatcaataac ctccagatta taatcgacgg caagaatatc
     1681 tgcgacatta agcttaagca gaaaatctac gaggacctgt ttaagaaata caagaagatc
     1741 acccagaagc agatttccac ctttatcaag catgagggca tttgtaacaa gacagacgag
     1801 gtgattatcc tgggtattga taaagaatgc actagttctc tgaagtctta catagaactt
     1861 aagaacatat ttgggaaaca agtcgatgag atatctacaa agaatatgct ggaggaaatc
     1921 atccggtggg ccacgatcta cgatgaaggg gagggaaaga caattctcaa gacaaaaatt
     1981 aaagccgagt acggcaaata ttgctcagac gaacagatta agaagatact caatctgaaa
     2041 ttctctggat ggggtagact tagccggaag tttttggaga cggtcaccag cgagatgcct
     2101 ggattttccg agccagttaa cataatcacc gcaatgagag aaacacagaa caacttgatg
     2161 gagcttcttt cctctgagtt cacatttacg gaaaacatta agaaaataaa tagtgggttt
     2221 gaggacgccg aaaagcagtt tagctacgac ggcctcgtta aacccctgtt tctcagtccc
     2281 agtgtcaaga agatgttgtg gcagacactg aagctggtga aagagatctc ccacataacc
     2341 caggccccgc ccaaaaagat ctttatcgaa atggcgaagg gcgctgagct cgaaccagcg
     2401 cggactaaaa cgagactgaa gattctccag gatttgtaca acaactgtaa gaacgacgcc
     2461 gatgccttta gttctgagat caaagacctt agcgggaaaa tagagaatga agacaatctt
     2521 aggctcagga gcgacaagtt gtacctctac tatacgcaac tcgggaaatg catgtactgc
     2581 ggaaagccca tagagattgg tcatgtgttc gacacttcca attatgacat cgatcatatc
     2641 tatccccagt ccaagatcaa agatgactca atctccaaca gagtgctggt atgctcctcc
     2701 tgtaacaaga acaaggaaga caagtacccg ttgaaatccg aaattcagag caagcagcgc
     2761 gggttttgga atttcctcca gcggaacaat ttcatctccc tggagaaatt gaatcgcctc
     2821 acgcgggcaa cccctatttc agatgacgag actgctaagt tcatcgcacg ccagctcgtg
     2881 gaaacacgcc aagctaccaa agtagccgct aaggtgctgg agaaaatgtt ccccgagact
     2941 aagatagttt acagtaaagc cgagactgtt tccatgttta ggaataaatt tgatatcgtt
     3001 aagtgtagag agataaatga tttccaccat gcacacgacg cgtacctgaa cattgtggtt
     3061 ggaaacgttt acaacaccaa gttcacaaac aatccatgga attttataaa agagaaaaga
     3121 gataacccca aaattgccga cacatacaac tactacaagg ttttcgacta cgatgtgaag
     3181 agaaacaaca tcacagcatg ggaaaagggg aagacgatta tcaccgtgaa ggatatgctg
     3241 aaaagaaaca ccccgatcta tacacggcag gccgcctgca agaaagggga gctgtttaac
     3301 cagacgatta tgaaaaaggg ccttggccag caccccctga aaaaggaggg acctttctca
     3361 aatatatcaa agtacggggg ctataacaag gtttccgccg cttactacac actcatcgaa
     3421 tacgaagaga aaggcaacaa gatcagatcc ctcgaaacca tccctctgta cctggtgaag
     3481 gacatacaga aagaccagga tgtgttgaag tcttatctta ccgacttgtt gggcaagaaa
     3541 gagttcaaaa ttctggttcc gaagatcaag atcaattctc tgctgaagat caacgggttc
     3601 ccgtgccaca ttacaggcaa gaccaacgat tcatttcttc tccgacctgc tgttcagttt
     3661 tgctgtagca ataacgaggt gctttacttt aaaaaaatca tccgattttc cgaaatccga
     3721 agccaaaggg agaagatcgg caagacaatt agcccttacg aagatttgtc cttcaggagc
     3781 tacatcaagg agaatctgtg gaagaaaacc aagaacgacg agatcggaga aaaggagttt
     3841 tacgatctcc tgcagaaaaa aaacctggag atatacgaca tgcttctgac aaaacacaag
     3901 gataccattt acaagaagcg tccaaactcc gcaactattg atattctggt taaaggtaag
     3961 gaaaagttta aaagcctgat catcgagaat cagtttgagg tgatcctgga gatcctgaaa
     4021 ctcttttctg ctacccggaa cgtgagcgac ctccagcaca ttggcggttc caagtacagc
     4081 ggggttgcca agatcgggaa taaaatctct tccctggata attgcattct gatctaccag
     4141 agcatcacag gcattttcga aaagaggatt gatctgttga aggtg
//
