LOCUS       sequence-195299-        4002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q927P4
FEATURES             Location/Qualifiers
     source          1..4002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaagaagc catatacaat tggactggat atcggtacta attctgtggg atgggcagtg
       61 ctcacagacc agtacgatct ggttaagaga aagatgaaaa tcgcaggcga ttctgagaag
      121 aagcagatca aaaaaaattt ttggggagtc aggcttttcg atgagggcca gactgctgca
      181 gatcgaagaa tggccaggac agctagacgc aggattgaac ggcgacggaa cagaatctct
      241 tacttgcaag ggatctttgc cgaggagatg agcaagaccg atgccaattt cttttgtagg
      301 cttagtgatt ctttctacgt agataacgaa aagcgcaata gccggcatcc cttctttgcg
      361 actatcgagg aggaggtgga ataccataaa aattacccca ccatttacca cttgagagag
      421 gaattggtca acagttctga aaaagctgac ctgcggcttg tgtatctggc ccttgcacac
      481 atcatcaagt atagaggcaa ttttctgatc gaaggcgccc tggatacaca aaacacttca
      541 gtggatggaa tctataaaca gtttatccag acatacaacc aggtatttgc tagcggcatc
      601 gaggacggca gcctcaaaaa actcgaggac aataaagacg tggccaaaat cctggtcgag
      661 aaagttaccc gaaaggagaa gctggagcgg atcctcaagc tgtatcctgg cgagaagtca
      721 gctggcatgt ttgcccagtt catcagcctg attgttggca gcaagggcaa ttttcaaaag
      781 ccatttgacc ttattgaaaa gtccgacata gagtgcgcca aggatagtta cgaggaggac
      841 ttggaatccc tgctcgccct cattggcgac gaatatgccg agctgtttgt ggcggcgaag
      901 aacgcttact cagcagtcgt gctgtcctcc atcattacag tagccgagac tgaaaccaac
      961 gccaagctgt ctgcaagtat gatagagcgc ttcgacacac acgaagagga cttgggagag
     1021 ctcaaagctt tcatcaaatt gcacctgcca aagcactacg aggaaatttt ttcaaataca
     1081 gaaaaacacg gctacgctgg atacattgac ggtaagacta agcaggcaga cttctacaaa
     1141 tacatgaaaa tgactttgga aaacatcgag ggcgctgatt actttattgc taaaattgaa
     1201 aaggaaaact tcctgcgcaa acagaggacg tttgataatg gagcaatacc gcatcaattg
     1261 catcttgagg agctggaggc tatactgcac cagcaggcca agtactaccc cttcctcaaa
     1321 gagaactacg ataaaatcaa gtcactcgta acatttcgga tcccatactt tgtagggcct
     1381 ctggctaacg gacaatccga gttcgcctgg ttgaccagga aagccgacgg ggaaatcagg
     1441 ccatggaaca tcgaggagaa ggttgatttt ggcaagagcg cagttgactt tatagagaag
     1501 atgacaaaca aggacaccta tctgcccaaa gaaaatgtct tgcctaagca tagtctctgc
     1561 tatcaaaagt acctggtgta caacgaactt actaaggtgc ggtatatcaa cgaccagggg
     1621 aagacctcat atttttccgg gcaagaaaag gagcagatat ttaatgactt gtttaagcag
     1681 aagagaaagg tcaagaaaaa ggatctggaa ttgttcttga gaaacatgtc acatgttgaa
     1741 tcccccacca tcgagggact cgaggattcc ttcaactcca gctacagtac gtaccacgac
     1801 ctgctgaagg tgggcatcaa acaggagatc ctcgacaatc ccgttaacac tgagatgctt
     1861 gaaaacattg ttaagatcct cactgttttc gaagataagc gcatgatcaa agagcaactt
     1921 cagcagttca gcgatgtgct ggacggggtt gtactcaaga aactggaaag gcggcactac
     1981 accggctggg gccgcctgtc cgccaaactg ctgatgggca tcagggacaa acagtctcac
     2041 cttacaatac tcgattatct gatgaatgac gatggcctca atcggaatct catgcagctc
     2101 atcaacgatt ctaatctctc tttcaagtcc atcattgaga aggagcaggt gaccacagcc
     2161 gacaaagaca tccagtcaat tgtcgctgac ctggcaggct ccccagctat taagaaaggc
     2221 atattgcaga gtctcaaaat agtggatgaa ctggtttctg tgatggggta ccctccacag
     2281 accatcgtgg tggaaatggc gcgcgagaac cagacaaccg ggaaaggaaa aaacaattcc
     2341 agacctagat ataaatccct ggagaaggcc atcaaggaat tcggaagcca aattctcaag
     2401 gagcacccca cagacaatca ggagctgagg aacaatcgcc tctacctgta ttacctccag
     2461 aacggcaagg atatgtacac cggtcaggat ctcgatatcc ataatctcag caattacgac
     2521 attgaccaca ttgtgcccca gtctttcatt acggacaata gtatcgacaa cctggtcctg
     2581 acctcttctg ctggtaaccg ggaaaaagga gacgacgttc ctcctctgga gatcgtgcgg
     2641 aaacgaaagg tcttttggga aaaactgtac cagggcaacc tgatgtccaa acgaaaattc
     2701 gattacctga cgaaggctga gaggggtgga cttacagaag ccgacaaagc tcggttcata
     2761 catagacagc tggtagagac aagacagatc accaagaacg tggcgaatat cctgcaccaa
     2821 cgcttcaatt acgagaagga tgaccatggt aacaccatga aacaggtgcg catcgtcaca
     2881 ctcaagtctg ccttggtcag ccagtttcgc aagcagttcc aactctataa ggtgagagat
     2941 gttaatgatt atcatcacgc ccacgatgca tacctgaacg gcgtggttgc taatacgctg
     3001 cttaaagtct atccacagct cgagcctgaa tttgtgtatg gagactacca ccagtttgat
     3061 tggtttaaag ccaacaaggc cactgcaaag aagcaattct acacgaacat catgctgttt
     3121 tttgcccaga aggataggat cattgacgag aacggggaga ttctgtggga taaaaaatac
     3181 ttggacaccg tcaagaaagt gatgagttat agacagatga acatcgtcaa aaagaccgag
     3241 atccagaagg gcgaattctc caaggcaacc atcaaaccca agggtaattc atcaaagctg
     3301 attccccgga aaacaaactg ggatccaatg aaatatgggg gcctggatag cccgaatatg
     3361 gcgtacgccg tggtgatcga gtacgcaaaa ggcaaaaaca agttggtgtt tgagaaaaaa
     3421 attatccgcg tgaccataat ggaaagaaaa gcctttgaga aagacgagaa agcatttctg
     3481 gaggagcagg gttacaggca acctaaggta ttggctaaac tccccaaata taccctgtat
     3541 gagtgtgaag aggggaggcg gagaatgctt gcgagcgcca acgaggccca gaagggcaac
     3601 cagcaagtgc tgcctaatca tttggtgacc ctgctgcatc atgctgccaa ttgtgaagtg
     3661 agtgacggga aatcactgga ttacatcgag tcaaatagag agatgtttgc cgagctcctc
     3721 gcccacgtct ctgagttcgc caagcgctat acactggccg aagccaatct caacaagatt
     3781 aaccagctgt tcgagcagaa taaggaaggt gatataaagg caatcgccca gtcatttgtc
     3841 gacctgatgg cattcaacgc aatgggagcg ccggcgagct tcaagttctt tgaaaccaca
     3901 attgagcgga agcggtacaa caacctgaag gaacttttga attccactat tatctaccag
     3961 agtatcaccg gcctttacga gagccgaaag aggctggatg at
//
