LOCUS       sequence-195300-        7036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  split YFP. Plant expression of ABI2-YFPn-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7036)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAM-35s-ABI2-YFPn-1
FEATURES             Location/Qualifiers
     source          1..7036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    923..947
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1041..1293)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1313..1864)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1906..2085)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2554..2898
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2842..2862
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     2900..2916
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2901..2917
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2933..2957
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4230..4254)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4262..4264,4265..4267,4268..4726)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(4307..4328)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4568..4587)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5018..5042
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5154..5863
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5936..6027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6028..6096,6097..6888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6246..6265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat ggactctagc tagaggatca caggcagcaa cgctctgtca tcgttacaat
      721 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
      781 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
      841 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
      901 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
      961 gacaacttaa taacacattg cggacgtttt taatgatcga atactaacgt ctctaccaga
     1021 tatcagcttg catgccggtc gatctagtaa catagatgac accgcgcgcg ataatttatc
     1081 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     1141 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     1201 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     1261 taagaaactt tattgccaaa tgtttgaacg atctgcttga ctctaggggt catcagattt
     1321 cggtgacggg caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac
     1381 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1441 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1501 cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca
     1561 gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg
     1621 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     1681 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     1741 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     1801 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     1861 tcatggtaga tcccctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag
     1921 gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg
     1981 accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa
     2041 actccagaaa cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg
     2101 taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc
     2161 tcatgatcag attgtcgttt cccgccttcg gtttgggcgc gcccggttca gaagaccaga
     2221 gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc
     2281 cagctatctg tcacttcatc gaaaggacag tagaaaagga agatggcttc tacaaatgcc
     2341 atcattgcga taaaggaaag gctatcgttc aagatgcctc taccgacagt ggtcccaaag
     2401 atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     2461 agcaagtgga ttgatgtgat acatggtgga gcacgacact ctcgtctact ccaagaatat
     2521 caaagataca gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc
     2581 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     2641 aaaggaagat ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     2701 tgcctctacc gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa
     2761 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
     2821 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     2881 atttcatttg gagaggacct cgaggtcgac ggtatcgata agcttcccat caacaagttt
     2941 gtacaaaaaa gcaggcttga tggacgaagt ttctcctgca gtcgctgttc cattcagacc
     3001 attcactgac cctcacgccg gacttagagg ctattgcaac ggtgaatcta gggttacttt
     3061 accggaaagt tcttgttctg gcgacggagc tatgaaagat tcttcctttg agatcaatac
     3121 aagacaagat tcattgacat catcatcatc tgctatggca ggtgtggata tctccgccgg
     3181 agatgaaatc aacggttcag atgagtttga tccgagatcg atgaatcaga gtgagaagaa
     3241 agtacttagt agaacagaga gtagaagtct gtttgagttc aagtgtgttc ctttatatgg
     3301 agtgacttcg atttgtggta gacgaccaga gatggaagat tctgtctcaa cgattcctag
     3361 attccttcaa gtttcttcta gttcgttgct tgatggtcga gtcactaatg gatttaatcc
     3421 tcacttgagt gctcatttct ttggtgttta cgatggccat ggcggttctc aggtagcgaa
     3481 ttattgtcgt gagaggatgc atctggcttt gacggaggag atagtgaagg agaaaccgga
     3541 gttttgtgac ggtgacacgt ggcaagagaa gtggaagaag gctttgttca actcttttat
     3601 gagagttgac tcggagattg aaactgtggc tcatgctccg gaaactgttg ggtctacctc
     3661 ggtggttgcg gttgtctttc cgactcacat ctttgtcgcg aattgcggcg actctagggc
     3721 ggttttgtgt cgcggcaaaa cgccactcgc gttgtcggtt gatcacaaac cggataggga
     3781 tgatgaagcg gcgaggatag aagctgccgg tgggaaagta atccggtgga acggggctcg
     3841 tgtatttggt gttctcgcaa tgtcaagatc cattggcgat agatacctta aaccgtcagt
     3901 aattccggat ccagaagtga cttcagtgcg gcgagtaaaa gaagatgatt gtctcatctt
     3961 agcaagtgat ggtctttggg atgtaatgac aaacgaagaa gtgtgcgatt tggctcggaa
     4021 acggatttta ctatggcata agaagaacgc gatggccgga gaggctttgc ttccggcgga
     4081 gaaaagagga gaaggaaaag atcctgcagc aatgtccgcg gcagagtatt tgtcgaagat
     4141 ggctttgcaa aaaggaagca aagacaatat aagtgtggta gtggttgatt tgaagggaat
     4201 aaggaaattc aagagcaaat ccttgaatta cccagctttc ttgtacaaag tggttgatgg
     4261 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4321 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     4381 cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac
     4441 cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
     4501 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4561 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     4621 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4681 caagctggag tacaactaca acagccacaa cgtctatatc atggcctaat ctagagtccg
     4741 caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt
     4801 gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc
     4861 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     4921 aattcctaaa accaaaatcc agtgaccggg cggccgccac cgcggtggag ggggatcaga
     4981 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
     5041 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     5101 tatccgttcg tccatttgta tgtgtacatc accgacgagc aaggcaagac cgagcgcctt
     5161 tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc
     5221 aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cggccgccgg cgttgtggat
     5281 acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg
     5341 ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt
     5401 ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga
     5461 tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta
     5521 ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg
     5581 cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt
     5641 tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta
     5701 taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg
     5761 gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg
     5821 ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc agtccttgcc
     5881 attgccggga tcggggcagt aacgggatgg gcgatcagcc cgaaagctac ccctatttgt
     5941 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6001 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6061 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6121 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6181 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6241 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6301 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6361 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6421 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6481 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6541 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6601 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6661 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6721 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6781 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6841 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6901 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6961 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7021 tgagcgtcag accccg
//
