LOCUS       sequence-195304-        5104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Name: pCBG68-RBPjk-Delta-A. RBPjk binding site A mutation in 
            pCBG68-LANApi (pDD2002)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Hilton IB, Dittmer DP
  TITLE     Quantitative analysis of the bidirectional viral G-protein-coupled 
            receptor and lytic latency-associated nuclear antigen promoter of 
            Kaposi's sarcoma-associated herpesvirus.
  JOURNAL   J Virol. 2012 Sep;86(18):9683-95. doi: 10.1128/JVI.00881-12. Epub 
            2012 Jun 27.
  PUBMED    22740392
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00881-12"; journalName: "J Virol"; date: "2012-09"; 
            volume: "86"; issue: "18"; pages: "9683-95"
COMMENT     Sequence Label: pDD2024
FEATURES             Location/Qualifiers
     source          1..5104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..92
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     41..60
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      475..484
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             481..2109
                     /codon_start=1
                     /product="green-emitting variant of click beetle 
                     luciferase"
                     /label=CBG68luc
                     /note="human codon-optimized"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHRHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RVGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSANIHSLRDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLEKAGG"
     RBS             1956..1964
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    2166..2287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2177..2196)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2231..2250
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2535..2552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2706..3294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2786..2805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3465..4256,4257..4325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4088..4107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4326..4430)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4457..4912
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4544..4563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4754..4775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5043..5091
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 aacatacgct ctctatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
       61 cagtgcaagt gcaggtgcca gaacatttct ctatcgatag gtaccgagct cttacgcgtg
      121 ctagcccggg ctgcaggaat tcgataggca gctgcgccac gaagcagtca cgtccccaag
      181 agcagcagct tggtccggct gacttataaa cagtgagtag cgcccgccca cttgtttcgg
      241 gcggcccgta aggcaccctt atctttgaaa tgctataaga tgtgtgggaa atagtaatac
      301 caggtgtaca tgatttgtgt taaggtgtgt ggtggttttc gaaaaacatg ggttattggc
      361 cgtttctgtt gtatacgccc ggattatata tctggacttg cctaggtagc atccatggca
      421 acgcaaggcc agcaaaccca ctatcaagct tggcattccg gtactgttgg taaagccacc
      481 atggtgaaac gcgaaaagaa cgtgatctac ggcccagaac cactgcatcc actggaagac
      541 ctcaccgctg gtgagatgct cttccgagca ctgcgtaaac ataggcacct ccctcaagca
      601 ctcgtggacg tcgtgggaga cgagagcctc tcctacaaag aatttttcga agctactgtg
      661 ctgttggccc aaagcctcca taattgtggg tacaaaatga acgatgtggt gagcatttgt
      721 gctgagaata acactcgctt ctttattcct gtaatcgctg cttggtacat cggcatgatt
      781 gtcgcccctg tgaatgaatc ttacatccca gatgagctgt gtaaggttat gggtattagc
      841 aaacctcaaa tcgtctttac taccaaaaac atcttgaata aggtcttgga agtccagtct
      901 cgtactaact tcatcaaacg catcattatt ctggataccg tcgaaaacat ccacggctgt
      961 gagagcctcc ctaacttcat ctctcgttac agcgatggta atatcgctaa tttcaagccc
     1021 ttgcattttg atccagtcga gcaagtggcc gctattttgt gctcctccgg caccactggt
     1081 ttgcctaaag gtgtcatgca gactcaccag aatatctgtg tgcgtttgat ccacgctctc
     1141 gaccctcgtg tgggtactca attgatccct ggcgtgactg tgctggtgta tctgcctttc
     1201 tttcacgcct ttggtttctc tattaccctg ggctatttca tggtcggctt gcgtgtcatc
     1261 atgtttcgtc gcttcgacca agaagccttc ttgaaggcta ttcaagacta cgaggtgcgt
     1321 tccgtgatca acgtcccttc agtcattttg ttcctgagca aatctccttt ggttgacaag
     1381 tatgatctga gcagcttgcg tgagctgtgc tgtggcgctg ctcctttggc caaagaagtg
     1441 gccgaggtcg ctgctaagcg tctgaacctc cctggtatcc gctgcggttt tggtttgact
     1501 gagagcactt ctgctaacat ccatagcttg cgagacgagt ttaagtctgg tagcctgggt
     1561 cgcgtgactc ctcttatggc tgcaaagatc gccgaccgtg agaccggcaa agcactgggc
     1621 ccaaatcaag tcggtgaatt gtgtattaag ggccctatgg tctctaaagg ctacgtgaac
     1681 aatgtggagg ccactaaaga agccattgat gatgatggct ggctccatag cggcgacttc
     1741 ggttactatg atgaggacga acacttctat gtggtcgatc gctacaaaga attgattaag
     1801 tacaaaggct ctcaagtcgc accagccgaa ctggaagaaa ttttgctgaa gaacccttgt
     1861 atccgcgacg tggccgtcgt gggtatccca gacttggaag ctggcgagtt gcctagcgcc
     1921 tttgtggtga aacaacccgg caaggagatc actgctaagg aggtctacga ctatttggcc
     1981 gagcgcgtgt ctcacaccaa atatctgcgt ggcggcgtcc gcttcgtcga ttctattcca
     2041 cgcaacgtta ccggtaagat cactcgtaaa gagttgctga agcaactcct cgaaaaagct
     2101 ggcggctagt aaagtcttca tgattatcta gagtcggggc ggccggccgc ttcgagcaga
     2161 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2221 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2281 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2341 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac
     2401 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     2461 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     2521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2881 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3121 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4321 ctcataccct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4441 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4501 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4561 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4621 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4681 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4741 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4801 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4861 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg
     4921 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     4981 cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc
     5041 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     5101 tact
//
