LOCUS       sequence-195329-       14360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Triple gRNA expression separated with 28nt Csy4 motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14360)
  AUTHORS   Ferreira R, Skrekas C, Nielsen J, David F
  TITLE     Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation using 
            Csy4 in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259.
  PUBMED    29161506
REFERENCE   2  (bases 1 to 14360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259."
COMMENT     Sequence Label: pCRA03
FEATURES             Location/Qualifiers
     source          1..14360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3039..3059
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3084..3233
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3258..3278
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3708..4064
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             4083..4652
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     protein_bind    complement(4657..4681)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4714..4730)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4715..4731)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      4733..4980
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4749..4767)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4998..5017)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4999..5017)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5027..5044)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5027..5043)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5036..5058)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5188..5643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5275..5294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5485..5506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5774..6577)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6578..6798)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6824..6843)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6943..6965
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7003..7021)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7063..7566
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7593..7697
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7698..7766,7767..8558)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7916..7935)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8729..9317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9218..9237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    9701..9719
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9785..9804
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        9833..9907
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    9912..9931
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        9960..10034
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10039..10058
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        10087..10161
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10166..10185
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     terminator      10194..10256
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     CDS             11407..12030
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(11480..11499)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     12527..12544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(12697..12702,12703..12720,12721..12727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12735..12751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12740..12762
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12759..12775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12759..12775
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12794..12814
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12796..12814
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12832..13230
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             13253..13273
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(13283..13309,13310..13312,13313..14360,1..1436,
                     1437..1442,1443..1508,1509..1511,1512..3026)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(13283..13309,13310..13312,13313..14360,1..1439,
                     1440..1442,1443..3026)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(13283..14360,1..3026)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct
       61 tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc
      121 gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc
      181 acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga
      241 ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt
      301 ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg aacttcgagg
      361 aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata
      421 aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag
      481 tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc
      541 tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta
      601 ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac tctgttgaaa
      661 tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa
      721 tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg
      781 tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg
      841 ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg
      901 ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc
      961 tggattttct taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg
     1021 actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc
     1081 ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga
     1141 ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg
     1201 ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa
     1261 ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc
     1321 cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca
     1381 gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggatg
     1441 ctatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat
     1501 ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga
     1561 aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc
     1621 tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc
     1681 agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga
     1741 acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt
     1801 ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca
     1861 attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa
     1921 aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga
     1981 aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca
     2041 gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc
     2101 gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt
     2161 tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag
     2221 tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga
     2281 tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg
     2341 cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg
     2401 tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg
     2461 actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca
     2521 agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc
     2581 tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca
     2641 gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag ctgttcgtgg
     2701 aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag
     2761 tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata
     2821 agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg
     2881 cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa
     2941 aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa
     3001 tcgacctctc tcagctcggt ggagacagca gggctgaccc caagaagaag aggaaggtgg
     3061 aggccagcgg ttccggacgg gctgacgcat tggacgattt tgatctggat atgctgggaa
     3121 gtgacgccct cgatgatttt gaccttgaca tgcttggttc ggatgccctt gatgactttg
     3181 acctcgacat gctcggcagt gacgcccttg atgatttcga cctggacatg ctgattaact
     3241 ctagaagttc cggatctccg aaaaagaaac gcaaagttgg tagccagtac ctgcccgaca
     3301 ccgacgaccg gcaccggatc gaggaaaagc ggaagcggac ctacgagaca ttcaagagca
     3361 tcatgaagaa gtcccccttc agcggcccca ccgaccctag acctccacct agaagaatcg
     3421 ccgtgcccag cagatccagc gccagcgtgc caaaacctgc cccccagcct taccccttca
     3481 ccagcagcct gagcaccatc aactacgacg agttccctac catggtgttc cccagcggcc
     3541 agatctctca ggcctctgct ctggctccag cccctcctca ggtgctgcct caggctcctg
     3601 ctcctgcacc agctccagcc atggtgtctg cactggctca ggcaccagca cccgtgcctg
     3661 tgctggctcc tggacctcca caggctgtgg ctccaccagc ccctaaacct acacaggccg
     3721 gcgagggcac actgtctgaa gctctgctgc agctgcagtt cgacgacgag gatctgggag
     3781 ccctgctggg aaacagcacc gatcctgccg tgttcaccga cctggccagc gtggacaaca
     3841 gcgagttcca gcagctgctg aaccagggca tccctgtggc ccctcacacc accgagccca
     3901 tgctgatgga ataccccgag gccatcaccc ggctcgtgac aggcgctcag aggcctcctg
     3961 atccagctcc tgcccctctg ggagcaccag gcctgcctaa tggactgctg tctggcgacg
     4021 aggacttcag ctctatcgcc gacatggact tctccgcact gctgggtagc ggatcgggat
     4081 ctcgggattc cagggaaggg atgtttttgc cgaagcctga ggccggctcc gctattagtg
     4141 acgtgtttga gggccgcgag gtgtgccagc caaaacgaat ccggccattt catcctccag
     4201 gaagtccatg ggccaaccgc ccactccccg ccagcctcgc accaacacca accggtccag
     4261 tacatgagcc agtcgggtca ctgaccccgg caccagtccc tcagccactg gatccagcgc
     4321 ccgcagtgac tcccgaggcc agtcacctgt tggaggatcc cgatgaagag acgagccagg
     4381 ctgtcaaagc ccttcgggag atggccgata ctgtgattcc ccagaaggaa gaggctgcaa
     4441 tctgtggcca aatggacctt tcccatccgc ccccaagggg ccatctggat gagctgacaa
     4501 ccacacttga gtccatgacc gaggatctga acctggactc acccctgacc ccggaattga
     4561 acgagattct ggataccttc ctgaacgacg agtgcctctt gcatgccatg catatcagca
     4621 caggactgtc catcttcgac acatctctgt tttgaaaccc agctttcttg tacaaagtgg
     4681 tgatgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agtcatgtaa
     4741 ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa
     4801 ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
     4861 agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt
     4921 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc
     4981 ggccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     5041 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5101 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5161 tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg
     5221 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5281 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5341 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5401 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5461 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5521 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5581 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5641 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagggta
     5701 ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt
     5761 ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt
     5821 ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa
     5881 caataataat gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca
     5941 atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt
     6001 catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct
     6061 tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca
     6121 attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca
     6181 aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg
     6241 ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt
     6301 ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg
     6361 tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg
     6421 gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac
     6481 acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat
     6541 gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg
     6601 tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac
     6661 atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg
     6721 agattaccga atcaaaaaaa tttcaaagaa accgaaatca aaaaaaagaa taaaaaaaaa
     6781 atgatgaatt gaattgaaaa gctgtggtat ggtgcactct cagtacaatc tgctctgatg
     6841 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     6901 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     6961 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7021 ttttataggt taatgtcatg ataataatgg tttcttagga cggatcgctt gcctgtaact
     7081 tacacgcgcc tcgtatcttt taatgatgga ataatttggg aatttactct gtgtttattt
     7141 atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa gagttacgga
     7201 atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt actttacata
     7261 tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa cgataagtaa
     7321 aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg aaacaattcg
     7381 gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg gcgatccccc
     7441 tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc tttttttact
     7501 ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat ttttatagca
     7561 cgtgatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7621 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7681 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7741 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7801 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7861 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7921 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7981 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8041 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8101 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8161 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8221 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8281 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8341 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8401 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8461 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8521 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8581 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8641 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8701 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8761 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8821 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8881 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8941 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9001 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9061 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9361 ggccttttgc tcgggggatc tgccaattga acataacatg gtagttacat atactagtaa
     9421 tatggttcgg cacacattaa aagtataaaa actatctgaa ttacgaatta catatattgg
     9481 tcataaaaat caatcaatca tcgtgtgttt tatatgtctc ttatctaagt ataagaatat
     9541 ccatagttaa tattcactta cgctaccttt taacctgtaa tcattgtcaa caggatatgt
     9601 taacgaccca cattgataaa cgctagtatt tctttttcct cttcttattg gccggctgtc
     9661 tctatactcc cctatagtct gtttcttttc gtttcgattg tccctatcag tgatagagat
     9721 ggcgcacatg gtacgctgtg gtgctcgcgg ctgggaacga aactctggga gctgcgattg
     9781 gcaggttcac tgccgtatag gcagctaaga aacgccccag ttggcttggc tcgttttaga
     9841 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     9901 gtcggtggtg cgttcactgc cgtataggca gctaagaaaa gctgttgttc gataaatccg
     9961 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
    10021 gcaccgagtc ggtggtgcgt tcactgccgt ataggcagct aagaaataat ggcatcggag
    10081 gctcgggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
    10141 aaaagtggca ccgagtcggt ggtgcgttca ctgccgtata ggcagctaag aaaccatatc
    10201 caacttccaa tttaatcttt cttttttaat tttcacttat ttgcgataca gaaagaaaaa
    10261 agcgatagta actattgaat tttgtttgga tttggttaga ttagatatgg tttctcttta
    10321 tatttacatg ctaaaaatgg gctacaccag agatacataa ttagatatat atacgccagt
    10381 acaccttatc ggcccaagcc ttgtcccaag gcagcgtttt gttcttggaa acgctgccct
    10441 acacgttcgc tatgcttcaa gaacttttct gagcacttca tgatgcatgt ttgttcctta
    10501 ttggttagct ttgatgttgt gaagtcattg acacagtctg tgaaacatct ttctaccaga
    10561 ttagagtaca aacgcatgaa atccttcatt tgcttttgtt ccactacttt ttggaactct
    10621 tgttgttctt tcctgcaggt agtcgtgcaa tgtatgactt taagatttgt gagcaggaag
    10681 aaaagggaga atcttctaac gataaaccct tgaaaaactg ggtagactac gctatgttga
    10741 gttgctacgc aggctgcaca attacacgag aatgctcccg cctaggattt aaggctaagg
    10801 gacgtgcaat gcagacgaca gatctaaatg accgtgtcgg tgaagtgttc gccaaacttt
    10861 tcggttaaca catgcagtga tgcacgcgcg atggtgctaa gttacatata tatatatata
    10921 tatatatata tatatatata gccatagtga tgtctaagta acctttatgg tatatttctt
    10981 aatgtggaaa gatactagcg cgcgcaccca cacacaagct tcgtcttttc ttgaagaaaa
    11041 gaggaagctc gctaaatggg attccacttt ccgttccctg ccagctgatg gaaaaaggtt
    11101 agtggaacga tgaagaataa aaagagagat ccactgaggt gaaatttcag ctgacagcga
    11161 gtttcatgat cgtgatgaac aatggtaacg agttgtggct gttgccaggg agggtggttc
    11221 tcaactttta atgtatggcc aaatcgctac ttgggtttgt tatataacaa agaagaaata
    11281 atgaactgat tctcttcctc cttcttgtcc tttcttaatt ctgttgtaat taccttcctt
    11341 tgtaattttt tttgtaatta ttcttcttaa taatccaaac aaacacacat attacaatat
    11401 attaaaatgt ctagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat
    11461 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag
    11521 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag
    11581 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt
    11641 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa
    11701 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc
    11761 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg
    11821 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg
    11881 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat
    11941 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa
    12001 aaacaactta aatgtgaaag tgggtcttaa gtctgaagaa tgaatgattt gatgatttct
    12061 ttttccctcc atttttctta ctgaatatat caatgatata gacttgtata gtttattatt
    12121 tcaaattaag tagctatata tagtcaagat aacgtttgtt tgacacgatt acattattcg
    12181 tcgacatctt ttttcagcct gtcgtggtag caatttgagg agtattatta attgaatagg
    12241 ttcattttgc gctcgcataa acagttttcg tcagggacag tatgttggaa tgagtggtaa
    12301 ttaatggtga catgacatgt tatagcaata accttgatgt ttacatcgta gtttaatgta
    12361 caccccgcga attcgttcaa gtaggagtgc accaattgca aagggaaaag ctgaatgggc
    12421 agttcgaacc gcggtctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    12481 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    12541 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    12601 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    12661 taatgtgagt tacctcactc attaggcacc ccaggcttta cactttatgc ttccggctcc
    12721 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
    12781 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tcatagcttc
    12841 aaaatgtttc tactcctttt ttactcttcc agattttctc ggactccgcg catcgccgta
    12901 ccacttcaaa acacccaagc acagcatact aaatttcccc tctttcttcc tctagggtgt
    12961 cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga gaccgcctcg tttctttttc
    13021 ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa attttttttt
    13081 tgattttttt ctctttcgat gacctcccat tgatatttaa gttaataaac ggtcttcaat
    13141 ttctcaagtt tcagtttcat ttttcttgtt ctattacaac tttttttact tcttgctcat
    13201 tagaaagaaa gcatagcaat ctaatctaag ttttctagag atgtctagag ccccaaagaa
    13261 gaagagaaaa gttagacccg ggatggacaa gaagtactcc attgggctcg ctatcggcac
    13321 aaacagcgtc ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa
    13381 agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt
    13441 cgactccggg gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac
    13501 ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt
    13561 ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca
    13621 cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc
    13681 aaccatatat catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt
    13741 gatctatctc gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga
    13801 cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa
    13861 tcagcttttc gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag
    13921 cgctaggctg tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa
    13981 gaagaacggc ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa
    14041 atctaacttc gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga
    14101 tgatctcgac aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc
    14161 aaagaacctg tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac
    14221 caaagctccg ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac
    14281 tttgctgaag gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga
    14341 tcagtctaaa aatggctacg
//
