LOCUS       sequence-195419-       11051 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-1249-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 11051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-1249-5p
FEATURES             Location/Qualifiers
     source          1..11051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(87..675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(167..186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(846..1637,1638..1706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1469..1488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1707..1778)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    1798..1835
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1857..2456
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1857..1876)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2353..2373
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    2612..2667
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    3646..3666
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3715..4893
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3946..4884
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    4910..4934
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4945..4954
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4951..5643
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     protein_bind    complement(5666..5690)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5987..6042
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    6426..6451
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    7123..7143
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        7192..8370
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7423..8361
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    8387..8411
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8413..8422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8419..8421,8422..8424,8425..9138)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(8464..8485)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(9143..9167)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9322..9377
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9738..9754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9738..9754)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9751..9773)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9762..9778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9786..9792,9793..9810,9811..9816))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9831..9852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10066..10085)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             10077..10886
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10144..10163)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aattgagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
       61 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
      961 ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca
     1021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1141 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1441 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1681 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1741 cagggttatt gtctcatgag cggatacata tttgaatgta gctccgaatt tctcgagggc
     1801 ttgtcgacga cggcggtctc cgtcgtcagg atcatgaatt cgacccgcct gccaccatga
     1861 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     1921 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     1981 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     2041 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     2101 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     2161 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     2221 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     2281 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     2341 catccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     2401 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatacg
     2461 cgtgaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     2521 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     2581 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     2641 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     2701 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     2761 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     2821 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     2881 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     2941 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     3001 ctgcagccca agcttgaatt ctctagaatt accctgttat ccctatgctt ggtttaccga
     3061 gctcttattg gttttcaaac ttcattgact gtgcccaacg cgcctgggag ctcacgggga
     3121 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     3181 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
     3241 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
     3301 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct aggctgaaag
     3361 accggttctt agaatgacac gcgcctggga gctcacgggg acagcccccc cccaaagccc
     3421 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     3481 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     3541 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     3601 cagacacctg gggggatacg gggaaaaagc taacttctga gacgacaact ttgtatagaa
     3661 aagttggctc cgaattcgcc cttgacagca gagatccagt ttggttaatt aattggctcc
     3721 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt gtggggaggg
     3781 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     3841 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     3901 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     3961 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     4021 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     4081 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     4141 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     4201 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     4261 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     4321 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     4381 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     4441 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     4501 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     4561 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     4621 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     4681 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     4741 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     4801 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     4861 caaagttttt ttcttccatt tcaggtgtcg tgagaagggc gaattcgacc caagtttgta
     4921 caaaaaagca ggctggatcc aggtgccacc atggtgagcg agctgattaa ggagaacatg
     4981 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag
     5041 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggcggt cgagggcggc
     5101 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag caaaaccttc
     5161 atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccccga gggcttcaca
     5221 tgggagagag tcaccacata cgaagatggg ggcgtgctga ccgctaccca ggacaccagc
     5281 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac
     5341 ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagac actgtacccc
     5401 gctgacggcg gcctggaagg cagagccgac atggccctga agctcgtggg cgggggccac
     5461 ctgatctgca accttaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg
     5521 cccggcgtct actatgtgga caggagactg gaaagaatca aggaggccga caaagagaca
     5581 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
     5641 cacaaactta attgagaatt ctaccaccca gctttcttgt acaaagtggt acgtctcaag
     5701 accagaggtc tctgcttcct cgtacaactt ggcccatctc ctccctccta acttggccca
     5761 tctcctccct cctaacttgg cccatctcct ccctcctaac ttggcccatc tcctccctcc
     5821 tcaaccagaa gagacctgtg cttcactcct caggtgcagg ctgcctatca gaaggtggtg
     5881 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     5941 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     6001 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     6061 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     6121 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     6181 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     6241 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     6301 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     6361 tggagatccc gagcggtgcg tttttatgct tgtagtattg tataatgttt ttaagatcca
     6421 agcttctgac ctcttctctt cctcccacag ggcctcgaga caattgcatc gatggtacca
     6481 gtgaaagtct gcaatgaatt cgagttggcg cgcctgtcat tctaaatctc tctttcagcc
     6541 taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     6601 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     6661 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     6721 ccctagcggg ggagggacgt aattacatcc ctgggggctt tgggaggggg ctgtccccgt
     6781 gagctcccag gcgcgcctgt cattctaaat ctctctttca gcctaaagct ttttccccgt
     6841 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
     6901 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
     6961 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
     7021 cgtaattaca tccctggggg ctttgggggg gggctgtccc cgtgagctcc caggcgcgcc
     7081 aactcggtac cgtatcgatg tcgacgttaa cgctagtgat atcaactttg tatagaaaag
     7141 ttggctccga attcgccctt gacagcagag atccagtttg gttaattaat tggctccggt
     7201 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttgtg gggaggggtc
     7261 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     7321 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     7381 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     7441 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     7501 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     7561 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     7621 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     7681 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     7741 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     7801 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     7861 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     7921 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     7981 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     8041 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     8101 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     8161 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     8221 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     8281 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     8341 agtttttttc ttccatttca ggtgtcgtga gaagggcgaa ttcgacccaa gtttgtacaa
     8401 aaaagcaggc tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     8461 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgag gggcgagggc
     8521 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     8581 cccgtgccct ggcccaccct cgtgaccacc ctgagccacg gcgtgcagtg cttcgcccgc
     8641 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     8701 caggagcgca ccatcttctt caaggacgac ggcacctaca agacccgcgc cgaggtgaag
     8761 ttcgagggcg acaccctagt gaaccgcatc gagctgaagg gcgtcgactt caaggaggac
     8821 ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaacat ctatatcatg
     8881 gccgtcaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa cgtggaggac
     8941 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     9001 ctgctgcccg acagccacta cctgagcacc cagtccgtgc tgagcaaaga ccccaacgag
     9061 aagcgcgatc acatggtcct gctggagttc cgcaccgccg ccgggatcac tctcggcatg
     9121 gacgagctgt acaagtaata ccacccagct ttcttgtaca aagtggtacg cgtgaattca
     9181 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     9241 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     9301 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     9361 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     9421 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     9481 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     9541 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     9601 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     9661 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     9721 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9781 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9841 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9901 cagcggatcg acagtactaa gcttggtcta atacccaatc tctcgtctta tccagatgtt
     9961 ttatacgcct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaataa
    10021 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
    10081 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
    10141 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
    10201 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
    10261 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
    10321 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
    10381 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
    10441 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
    10501 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
    10561 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
    10621 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
    10681 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
    10741 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
    10801 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
    10861 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
    10921 gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt
    10981 acgcgtcgtt ccactgagcg tcagaggtat cactagtatt acagaggtaa gttataacag
    11041 tcgcctaacc c
//
