LOCUS       sequence-19542-e         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF108
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(207..224)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    213..437
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggcttatcga aattaatacg actcactata gggagaccca agctggctag cgtttaaacg
       61 ggccctctag acccggggag aagccttaca cgtgcagcga ctgcggcaag gcatttcgcg
      121 acaagtcctg cctgaaccgc cacagacgca cccacaccgg tggatcctct gcacagtagt
      181 aagcttaagt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
      241 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
      301 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
      361 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
      421 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtaccc
      481 cacgcgccct gtagcggcgc attaaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      541 cgctacactt gccagcg
//
