LOCUS       sequence-19547-e         508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF113
FEATURES             Location/Qualifiers
     source          1..508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(214..231)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    220..444
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgt
       61 ttaaacgggc cctctagacc cggggagaag ccttatggct gccatctgtg tggcaaggcg
      121 ttcagcaaat ctagcaacct gcgccgtcac gagatgatcc ataccggtgg atcctctgca
      181 cagtagtaag cttaagttta aaccgctgat cagcctcgac tgtgccttct agttgccagc
      241 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      301 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
      361 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
      421 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
      481 ggtatcccca cgcgccctgt agcggcgc
//
