LOCUS       sequence-19548-e         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF114
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(224..241)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    230..456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 tggctagcgt ttaaacgggc cctctagacc cggggagaag ccttacaagt gcaaagagtg
      121 cggccaggcg ttccgccagc gtgcccacct gattcgtcac cacaagctgc ataccggtgg
      181 atcctctgca cagtagtaag cttaagttta aaccgctgat cagcctcgac tgtgccttct
      241 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
      301 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
      361 cattctattc tgggggggtg ggggtggggc aggacagcaa gggggaggat tgggaagaca
      421 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
      481 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      541 tggttacgcg cagcgtgacc gctacacttg c
//
