LOCUS       sequence-19553-e         406 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF119
FEATURES             Location/Qualifiers
     source          1..406
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(237..254)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    243..354
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tctctggcta ctagagaacc cctgcttact ggcttatcga aattaatacg actcactata
       61 gggagaccca agctggctag cgtttaaacg ggccctctag acccggggag aagccttaca
      121 agtgcgagga gtgcggcaag gctttccggc aaagctccca cctcacaacc cacaagatca
      181 tccacaccgg tggatcctct gcacagtagt aagcttaagt ttaaaccgct gatcagcctc
      241 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      301 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
      361 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggac
//
