LOCUS       sequence-19564-e         505 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF130
FEATURES             Location/Qualifiers
     source          1..505
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(239..256)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    245..469
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct ggctagcgtt taaacgggcc ctctagaccc ggggagaagc cttacgtgtg
      121 cgacgtggaa ggctgtacat ggaagtttgc acggtccgat gaactcaacc ggcacaagaa
      181 gcggcacacc ggtggatcct ctgcacagta gtaagcttaa gtttaaaccg ctgatcagcc
      241 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
      301 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
      361 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacan caagggggag
      421 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctggagcg
      481 gaaagaaccg gctggggctc taggg
//
