LOCUS       sequence-19565-e         531 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF131
FEATURES             Location/Qualifiers
     source          1..531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(187..204)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    193..417
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cactataggg agagccaagc tggctagcgt ttaaacgggc cctctagacc cggggagaag
       61 cctttcctgt gccagtattg cgcccagcgt ttcggtcgca aggaccactt aacgcgccac
      121 atgaagaaga gccataccgg tggatcctct gcacagtagt aagcttaagt ttaaaccgct
      181 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
      241 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
      301 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
      361 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
      421 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
      481 cattaagcgc ggcgggtgtg gtgggttacg cgcagcgtga ccgctacact t
//
