LOCUS       sequence-19567-e         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF133
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(251..268)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    257..481
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcagagctc tctggctact agagacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaacggg ccctctagac ccggggagaa
      121 gccttacgtc tgcgacgtgg agggctgcac gtggaagttc gccaggtccg acaagctcaa
      181 ccggcacaag aagcgccaca ccggtggatc ctctgcacag tagtaagctt aagtttaaac
      241 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      301 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      361 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      421 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      481 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
      541 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      601 gccctagcgc ccgctccttt tgctttcttc cctt
//
