LOCUS       sequence-195703-       11047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-19b-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 11047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-19b-3p
FEATURES             Location/Qualifiers
     source          1..11047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(87..675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(167..186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(846..1637,1638..1706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1469..1488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1707..1778)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    1798..1835
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1857..2456
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1857..1876)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2353..2373
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    2612..2667
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    3646..3666
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3715..4893
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3946..4884
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    4910..4934
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4945..4954
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4951..5643
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     protein_bind    complement(5666..5690)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5983..6038
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    6422..6447
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    7119..7139
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        7188..8366
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7419..8357
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    8383..8407
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8409..8418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8415..8417,8418..8420,8421..9134)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(8460..8481)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(9139..9163)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9318..9373
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9734..9750)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9734..9750)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9747..9769)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9758..9774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9782..9788,9789..9806,9807..9812))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9827..9848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10062..10081)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             10073..10882
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10140..10159)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aattgagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
       61 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
      961 ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca
     1021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1141 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1441 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1681 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1741 cagggttatt gtctcatgag cggatacata tttgaatgta gctccgaatt tctcgagggc
     1801 ttgtcgacga cggcggtctc cgtcgtcagg atcatgaatt cgacccgcct gccaccatga
     1861 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     1921 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     1981 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     2041 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     2101 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     2161 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     2221 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     2281 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     2341 catccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     2401 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatacg
     2461 cgtgaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     2521 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     2581 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     2641 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     2701 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     2761 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     2821 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     2881 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     2941 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     3001 ctgcagccca agcttgaatt ctctagaatt accctgttat ccctatgctt ggtttaccga
     3061 gctcttattg gttttcaaac ttcattgact gtgcccaacg cgcctgggag ctcacgggga
     3121 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     3181 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
     3241 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
     3301 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct aggctgaaag
     3361 accggttctt agaatgacac gcgcctggga gctcacgggg acagcccccc cccaaagccc
     3421 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     3481 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     3541 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     3601 cagacacctg gggggatacg gggaaaaagc taacttctga gacgacaact ttgtatagaa
     3661 aagttggctc cgaattcgcc cttgacagca gagatccagt ttggttaatt aattggctcc
     3721 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt gtggggaggg
     3781 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     3841 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     3901 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     3961 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     4021 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     4081 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     4141 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     4201 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     4261 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     4321 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     4381 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     4441 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     4501 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     4561 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     4621 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     4681 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     4741 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     4801 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     4861 caaagttttt ttcttccatt tcaggtgtcg tgagaagggc gaattcgacc caagtttgta
     4921 caaaaaagca ggctggatcc aggtgccacc atggtgagcg agctgattaa ggagaacatg
     4981 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag
     5041 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggcggt cgagggcggc
     5101 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag caaaaccttc
     5161 atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccccga gggcttcaca
     5221 tgggagagag tcaccacata cgaagatggg ggcgtgctga ccgctaccca ggacaccagc
     5281 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac
     5341 ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagac actgtacccc
     5401 gctgacggcg gcctggaagg cagagccgac atggccctga agctcgtggg cgggggccac
     5461 ctgatctgca accttaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg
     5521 cccggcgtct actatgtgga caggagactg gaaagaatca aggaggccga caaagagaca
     5581 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
     5641 cacaaactta attgagaatt ctaccaccca gctttcttgt acaaagtggt acgtctcaag
     5701 accagaggtc tctgcttctg atatgtcagt tttgcatgga tttgcacatc agttttgcat
     5761 ggatttgcac atcagttttg catggatttg cacatcagtt ttgcatggat ttgcacacaa
     5821 ccagaagaga cctgtgcttc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
     5881 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     5941 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     6001 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     6061 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     6121 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     6181 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     6241 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     6301 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     6361 gatcccgagc ggtgcgtttt tatgcttgta gtattgtata atgtttttaa gatccaagct
     6421 tctgacctct tctcttcctc ccacagggcc tcgagacaat tgcatcgatg gtaccagtga
     6481 aagtctgcaa tgaattcgag ttggcgcgcc tgtcattcta aatctctctt tcagcctaaa
     6541 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     6601 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     6661 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     6721 agcgggggag ggacgtaatt acatccctgg gggctttggg agggggctgt ccccgtgagc
     6781 tcccaggcgc gcctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc
     6841 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
     6901 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
     6961 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
     7021 attacatccc tgggggcttt gggggggggc tgtccccgtg agctcccagg cgcgccaact
     7081 cggtaccgta tcgatgtcga cgttaacgct agtgatatca actttgtata gaaaagttgg
     7141 ctccgaattc gcccttgaca gcagagatcc agtttggtta attaattggc tccggtgccc
     7201 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgtgggga ggggtcggca
     7261 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     7321 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     7381 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     7441 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     7501 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     7561 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     7621 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     7681 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     7741 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     7801 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     7861 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     7921 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     7981 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     8041 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     8101 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     8161 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     8221 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     8281 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     8341 tttttcttcc atttcaggtg tcgtgagaag ggcgaattcg acccaagttt gtacaaaaaa
     8401 gcaggctcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     8461 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgaggggc gagggcgagg
     8521 gcgatgccac caacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     8581 tgccctggcc caccctcgtg accaccctga gccacggcgt gcagtgcttc gcccgctacc
     8641 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     8701 agcgcaccat cttcttcaag gacgacggca cctacaagac ccgcgccgag gtgaagttcg
     8761 agggcgacac cctagtgaac cgcatcgagc tgaagggcgt cgacttcaag gaggacggca
     8821 acatcctggg gcacaagctg gagtacaact tcaacagcca caacatctat atcatggccg
     8881 tcaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacgtg gaggacggca
     8941 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     9001 tgcccgacag ccactacctg agcacccagt ccgtgctgag caaagacccc aacgagaagc
     9061 gcgatcacat ggtcctgctg gagttccgca ccgccgccgg gatcactctc ggcatggacg
     9121 agctgtacaa gtaataccac ccagctttct tgtacaaagt ggtacgcgtg aattcactcc
     9181 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     9241 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     9301 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     9361 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     9421 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     9481 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     9541 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     9601 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     9661 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     9721 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     9781 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     9841 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     9901 ggatcgacag tactaagctt ggtctaatac ccaatctctc gtcttatcca gatgttttat
     9961 acgcctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaataatatc
    10021 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
    10081 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
    10141 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
    10201 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
    10261 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
    10321 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
    10381 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
    10441 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
    10501 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
    10561 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
    10621 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
    10681 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
    10741 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
    10801 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
    10861 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
    10921 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
    10981 gtcgttccac tgagcgtcag aggtatcact agtattacag aggtaagtta taacagtcgc
    11041 ctaaccc
//
