LOCUS       sequence-19627-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  TITLE     de Lange Lab Vectors
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLPC-N FLAG
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..131
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatcagc caccatggac tacaaagacg
      121 atgacgataa aggatccact agtaacggcc gccagtgtgc tggaattctg cagatatcca
      181 tcacactggc ggccgctcga gcatgcatct agagggccga tgaaataaaa gattttattt
      241 agtctccaga agaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
      301 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
      361 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      421 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
      481 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
      541 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc ccaggacctg
      601 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
      661 gcttctgctc cc
//
