LOCUS       sequence-19629-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  TITLE     de Lange Lab Vectors
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWZL Hygro-N FLAG
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     169..191
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     207..223
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      250..259
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             259..282
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(431..448)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     658..677
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
       61 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
      121 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
      181 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg
      241 gccggatcag ccaccatgga ctacaaagac gatgacgata aaggatccca gtgtggtggt
      301 acgtaggaat tcgccagcac agtggtcgac caattccggt tattttccac catattgccg
      361 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
      421 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
      481 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
      541 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
      601 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
      661 ctctcctcaa gcgtattcaa ca
//
