LOCUS       sequence-19633-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Murphy KC, Campellone KG
  TITLE     Lambda Red-mediated recombinogenic engineering of enterohemorrhagic 
            and enteropathogenic E. coli.
  JOURNAL   BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.1186/1471-2199-4-11.
  PUBMED    14672541
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2003-12-13"; volume: "4:11. doi"; pages: " 
            10.1186/1471-2199-4-11"
COMMENT     Sequence Label: pKM201
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(75..80,81..96,97..103)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    111..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     116..138
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             160..576
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
ORIGIN
        1 ttgatatcga attcctgcag cccgggggat ctctgacatc ataacggttc tggcaaatat
       61 tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg
      121 ataacaattt cacacaggaa acagtcgacg cttataaaaa tggatattaa tactgaaact
      181 gagatcaagc aaaagcattc actaaccccc tttcctgttt tcctaatcag cccggcattt
      241 cgcgggcgat attttcacag ctatttcagg agttcagcca tgaacgctta ttacattcag
      301 gatcgtcttg aggctcagag ctgggcgcgt cactaccagc agctcgcccg tgaagagaaa
      361 gaggcagaac tggcagacga catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta
      421 tgcatcgatc atttgcaacg ccacggggcc agcaaaaaat ccattacccg tgcgtttgat
      481 gacgatgttg agtttcagga gcgcatggca gaacacatcc ggtacatggt tgaaaccatt
      541 gctcaccacc aggttgatat tgattcagag gtataaaacg aatgagtact gcactcgcaa
      601 cgctggctgg gaagctggct gaacgtgtcg gcatggattc tgtcgaccca caggaactga
      661 tcaccactct tcgccagacg gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat
      721 tactgatcgt tgccaaccag tacggcctta atccgtggac gaaagaaatt tacgcctttc
      781 ctgataagca gaatggcatc gtt
//
