LOCUS       sequence-19634-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Murphy KC, Campellone KG
  TITLE     Lambda Red-mediated recombinogenic engineering of enterohemorrhagic 
            and enteropathogenic E. coli.
  JOURNAL   BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.1186/1471-2199-4-11.
  PUBMED    14672541
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2003-12-13"; volume: "4:11. doi"; pages: " 
            10.1186/1471-2199-4-11"
COMMENT     Sequence Label: pKM200
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..48
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     37..59
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             81..497
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
ORIGIN
        1 aantaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga
       61 aacagtcgac gcttataaaa atggatatta atactgaaac tgagatcaag caaaagcatt
      121 cactaacccc ctttcctgtt ttcctaatca gcccggcatt tcgcgggcga tattttcaca
      181 gctatttcag gagttcagcc atgaacgctt attacattca ggatcgtctt gaggctcaga
      241 gctgggcgcg tcactaccag cagctcgccc gtgaagagaa agaggcagaa ctggcagacg
      301 acatggaaaa aggcctgccc cagcacctgt ttgaatcgct atgcatcgat catttgcaac
      361 gccacggggc cagcaaaaaa tccattaccc gtgcgtttga tgacgatgtt gagtttcagg
      421 agcgcatggc agaacacatc cggtacatgg ttgaaaccat tgctcaccac caggttgata
      481 ttgattcaga ggtataaaac gaatgagtac tgcactcgca acgctggctg ggaagctggc
      541 tgaacgtgtc ggcatggatt ctgtcgaccc acaggaactg atcaccactc ttcgccagac
      601 ggcatttaaa ggtgatgcca gcgatgcgca gttcatcgca ttactgatcg ttgccaacca
      661 gtacggcctt aatccgtgga cgaaagaaat ttacgccttt cctgataagc agaatggcat
      721 cgttccggtg gtgggcgttg atggctggtc ccgcatcatc aatgaaaacc agcagtttga
      781 tggcatgg
//
