LOCUS       sequence-19652-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Murphy KC, Campellone KG
  TITLE     Lambda Red-mediated recombinogenic engineering of enterohemorrhagic 
            and enteropathogenic E. coli.
  JOURNAL   BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.1186/1471-2199-4-11.
  PUBMED    14672541
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2003-12-13"; volume: "4:11. doi"; pages: " 
            10.1186/1471-2199-4-11"
COMMENT     Sequence Label: pTP223
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(114..136)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    125..141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(149..155,156..173,174..179))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 atcgcccgcg aaatgccggg ctgattagga aaacaggaaa gggggttagt gaatgctttt
       61 gcttgatctc agtttcagta ttaatatcca ttttttataa gcgtcgactg tttcctgtgt
      121 gaaattgtta tccgctcaca attccacaca ttatacgagc cggaagcata aagtgtaaag
      181 cctggggtgc ctaatgagtg agaattctat gctcctatat tgataagaat aaacttaata
      241 cgataaatga ggtattaggg atttaattat tctttattga tataaaaagt cctagcaatc
      301 caaatgggat tgctaggacc aaacaaaata gattatatag cataaatagg tttaattttg
      361 ctacgggggc gttatttaga ttttttcttc tttcgaaaaa atctttcttt atgaagttaa
      421 aagctatgtg ttcaatagca tattttgaat atggacatag aatagtgctt atcactattg
      481 catatagcat cttatctgtc acaaggaaat aatactcttc gctgtttttt gttataaggt
      541 atatatatat aagtgtgcag tacaggccaa ataaaatatt ttttatgtag tatcttaagc
      601 tcataaatta aacctcgtca tatattcttt tcattttata aggatcgagt tatgaggaaa
      661 agattttttg tgggaatatt cgcgataaac ctccttgttg gatgtcaggc taactatata
      721 cgtgatgttc agggagggac tgtggctcca tcatcttctt ctgaattggt ggggatcacc
      781 gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      841 gcttgcaaac aaaaaaacca ccg
//
