LOCUS       sequence-19655-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Wang W, Budhu A, Forgues M, Wang XW
  TITLE     Temporal and spatial control of nucleophosmin by the Ran-Crm1 
            complex in centrosome duplication.
  JOURNAL   Nat Cell Biol. 2005 Aug . 7(8):823-30.
  PUBMED    16041368
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2005 Aug . 7(8):823-30."
COMMENT     Sequence Label: GFP-NPM NLS1D
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    31..96
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             109..132
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccggnccnt cggcangacg agctgtcaag tccggactca gatctcgagc tcaagcttcg
       61 aattctgcag tcgacggtac cgcgggcccg ggatccgccg ccaccatgga ctacaaggat
      121 gacgacgaca agcatatgga agattcgatg gacatggaca tgagccccct gaggccccag
      181 aactatcttt tcggttgtga actaaaggcc gacaaagatt atcactttaa ggtggataat
      241 gatgaaaatg agcaccagtt atctttaaga acggtcagtt taggggctgg tgcaaaggat
      301 gagttgcaca ttgttgaagc agaggcaatg aattacgaag gcagtccaat taaagtaaca
      361 ctggcaactt tgaaaatgtc tgtacagcca acggtttccc ttgggggctt tgaaataaca
      421 ccaccagtgg tcttaaggtt gaagtgtggt tcagggccag tgcatattag tggacagcac
      481 ttagtagctg tggaggaaga tgcagagtca gaagatgaag aggaggagga tgtgaagctt
      541 tctgcccctg gaggtggtag caaggttcca cagaaaaaag taaaacttgc tgctgatgaa
      601 gatgatgacg atgatgatga agaggatgat gatgaagatg atgatgatga tgattttgat
      661 gatgaggaag ctgaagaaaa agcgccagtg aagaaatcta tacgagatac cccccccnna
      721 tnnncccaaa gtcatcccag angggaaaat ancnacccnt cttcacccna tgtcaaagga
      781 cagatcttca gaacaggaaa actctaaaca caaagactag tctgtagaan ttaa
//
