LOCUS       sequence-19656-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Wang W, Budhu A, Forgues M, Wang XW
  TITLE     Temporal and spatial control of nucleophosmin by the Ran-Crm1 
            complex in centrosome duplication.
  JOURNAL   Nat Cell Biol. 2005 Aug . 7(8):823-30.
  PUBMED    16041368
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2005 Aug . 7(8):823-30."
COMMENT     Sequence Label: GFP-NPM NLS2D
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..95
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..131
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccgggccnt cggcangacg agctgtcagt ccggactcag atctcgagct caagcttcga
       61 attctgcagt cgacggtacc gcgggcccgg gatccgccgc caccatggac tacaaggatg
      121 acgacgacaa gcatatggaa gattcgatgg acatggacat gagccccctg aggccccaga
      181 actatctttt cggttgtgaa ctaaaggccg acaaagatta tcactttaag gtggataatg
      241 atgaaaatga gcaccagtta tctttaagaa cggtcagttt aggggctggt gcaaaggatg
      301 agttgcacat tgttgaagca gaggcaatga attacgaagg cagtccaatt aaagtaacac
      361 tggcaacttt gaaaatgtct gtacagccaa cggtttccct tgggggcttt gaaataacac
      421 caccagtggt cttaaggttg aagtgtggtt cagggccagt gcatattagt ggacagcact
      481 tagtagctgt ggaggaagat gcagagtcag aagatgaaga ggaggaggat gtgaaactct
      541 taagtatatc tggaaagcgg tctgcccctg gaggtggtag cgtaaagctt gctgctgatg
      601 aagatgatga cgatgatgat gaagaggatg atgatgaaga tgatgatgat gatgattttg
      661 atgatgagga agctgaaaaa aaagcgccag tgaagaaatc tatacgagat actccagccn
      721 aaaatgcaca aaagtcaaat cagaatggaa agactccaaa ccatcatcac acnagatcaa
      781 aggacagatc cncagaacag aaaaactcta ancccaaaga cttttngtna aaattaacna
      841 a
//
