LOCUS       sequence-19659-e        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Wang W, Budhu A, Forgues M, Wang XW
  TITLE     Temporal and spatial control of nucleophosmin by the Ran-Crm1 
            complex in centrosome duplication.
  JOURNAL   Nat Cell Biol. 2005 Aug . 7(8):823-30.
  PUBMED    16041368
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2005 Aug . 7(8):823-30."
COMMENT     Sequence Label: GFP-NPM NLS1/2D
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    22..87
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..123
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccggcatgga cgagctgtca gtccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccgcc gccaccatgg actacaagga tgacgacgac
      121 aagcatatgg aagattcgat ggacatggac atgagccccc tgaggcccca gaactatctt
      181 ttcggttgtg aactaaaggc cgacaaagat tatcacttta aggtggataa tgatgaaaat
      241 gagcaccagt tatctttaag aacggtcagt ttaggggctg gtgcaaagga tgagttgcac
      301 attgttgaag cagaggcaat gaattacgaa ggcagtccaa ttaaagtaac actggcaact
      361 ttgaaaatgt ctgtacagcc aacggtttcc cttgggggct ttgaaataac accaccagtg
      421 gtcttaaggt tgaagtgtgg ttcagggcca gtgcatatta gtggacagca cttagtagct
      481 gtggaggaag atgcagagtc agaagatgaa gaggaggagg atgtgaagct tgctgctgat
      541 gaagatgatg acgatgatga tgaagaggat gatgatgaag atgatgatga tgatgatttt
      601 gatgatgagg aagctgaaga aaaagcgcca gtgaagaaat ctatacgaga tactccagcc
      661 aaaaatgcac aaaagtcaaa tcagaatgga aaagactcaa aaccatcatc aacaccaaga
      721 tcaaaaggac aagaatcctt caagaaacag gaaaaaactc ctaaaacacc aaaaggacct
      781 agttctgtag aagacattaa agcaaaaatg caagcaagta tagaaaaagg tggttctctt
      841 cccaaagtgg aagccaaatt catcaattat gtgaagaatt gcttccggat gactgaccaa
      901 gaggctattc aagatctctg gcagtggaag gaagtctctt taaggatcca ccggatctag
      961 ataactgatc ataatcagcc ataccacatt tgtagaggtt tnctgttaac tccctcc
//
